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Pectin lyase overproduction by Penicillium griseoroseum mutants resistant to catabolite repression BJM
Lima,Juliana Oliveira; Pereira,Jorge Fernando; Araújo,Elza Fernandes de; Queiroz,Marisa Vieira de.
Abstract Expression of pectinolytic genes is regulated by catabolic repression limiting the production of pectin lyase (PL) if the natural inducer, pectin, is missing from the growth medium. Here, we report the isolation of Penicillium griseoroseum mutants resistant to 2-deoxy-d-glucose (DG) that show resistance to catabolite repression and overproduce PL. Three spontaneous and nine UV-induced mutants were obtained. Some mutants produced sectors (segments morphologically different) that were also studied. The mutants were analyzed for pectinases production on pectinase-agar plates and five mutants and two sectors showing larger clearing zones than the wild type were selected for quantitative assay. Although PL production higher than the wild type has been...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2-Deoxy-d-glucose; Ultraviolet mutagenesis; Pectinolytic complex; Spontaneous mutant.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300602
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