| For the purpose of acquiring a highly active producer of pectolytic enzymes, there was a probe of isolating 140 species of Aspergillus’s fungi from different substrates (soils, grape malts, grapes, apples and sugar beet shreds). The isolated kinds of fungi were kept on a slant agar surface according to Chapek, with 2% of pectin. Testing was performed on the isolated layers of fungi as to the production of pectinolytic enzymes. The nourishing base used was the synthetic Chapek base with 2% pectin, 2% lactose and 0.7 % (NH4)2HPO4, and a natural base of 1% refuse apple pulp. Within 48 hours after cultivating the fungi, the filtrates were tested by the viscozimetric method to determine their entire pectolytic activity. The acquired results showed that a... |
