| A total of 292 animals from three breeds (Alpine and Saanen dairy breeds, and the Brazilian naturalized breed Moxotó) were genotyped, comprising 276 paternity cases. Statistical analyses were carried out by using TFPGA and CERVUS programs. Heterozygosis ranged from 0.542 (ILSTS005) to 0.825 (INRA006), with an average of 0.717 for all loci. Polymorphic information content (PIC) was 0.676 and 0.542, and combined exclusion probabilities (EP) were 0.999591 and 0.988375 for known and unknown maternal genotypes, respectively. The microsatellite system reveals 10% of paternity misidentification in evaluated registers. |
