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	Provedor de dados BJID (1) BJM (1) Autor Almeida,Wendell Alexandre Pinheiro de (1) Ao-ri-ge-le, (1) Jia,De-gang (1) Lillehoj,H.S. (1) Pedrosa,Celia Maria Silva (1) Postnikoff,Andrew CL (1) Rocha,Eliana Maria Mauricio da (1) Si-mu-ji-de, (1) Wang,Chun-jie (1) Ximenes,Ricardo Arraes de Alencar (1) Xu,Si-ri-gu-leng (1) Yao,Hong-qiang (1) Yu,Siriguleng (1) Zhao,Huai-ping (1) Mais... Palavra-chave Peripheral blood (2) American visceral leishmaniasis (1) Bone marrow blood (1) Cell mediated immune competency (1) Diagnosis (1) Fowl adenovirus VIII (1) Immune system (1) Polymerase chain reaction (1) T lymphocyte subpopulations (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 2012 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Validity of the polymerase chain reaction in the diagnosis of clinically suspected cases of American visceral leishmaniasis BJID Pedrosa,Celia Maria Silva; Ximenes,Ricardo Arraes de Alencar; Almeida,Wendell Alexandre Pinheiro de; Rocha,Eliana Maria Mauricio da. To test the validity of the polymerase chain reaction for diagnosing American visceral leishmaniasis, 88 suspected cases were studied. Diagnosis was confirmed in 47 (53.5%) and ruled out in 41 (46.5%) patients. Samples of bone marrow and peripheral blood were processed by polymerase chain reaction to evaluate the sensitivity and specificity of the test and its agreement beyond chance with microscopy examination. The polymerase chain reaction was positive in bone marrow of 100% of the patients with amastigotes seen with microscopy examination, and in 59.5% in those where no parasite were seen. Agreement beyond chance between visualization of the parasite in bone marrow aspirates and polymerase chain reaction was considered weak (Kappa = 0.41). Concordance... Tipo: Info:eu-repo/semantics/article Palavras-chave: American visceral leishmaniasis; Diagnosis; Polymerase chain reaction; Peripheral blood; Bone marrow blood. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000300003 Regulation of T lymphocyte subpopulations in specific pathogen-free chickens following experimental fowl adenovirus-Ⅷinfection BJM Wang,Chun-jie; Yu,Siriguleng; Ao-ri-ge-le,; Jia,De-gang; Yao,Hong-qiang; Zhao,Huai-ping; Lillehoj,H.S.; Si-mu-ji-de,; Postnikoff,Andrew CL; Xu,Si-ri-gu-leng. Two-day-old specific pathogen-free (SPF) chickens were divided into two groups. Group I was inoculated orally with fowl adenovirus VIII (FAV-VIII). Group II served as a negative control. Chickens were investigated at various days post-inoculation (dpi) by flow cytometric analysis for changes in T lymphocyte subpopulations in immune system and blood. In the thymus, CD3+ T lymphocytes were increased at 25 dpi, with significant increases in the FAV infected noted at 1, 12, 20dpi (p<0.05). This was accompanied by a corresponding increase of CD4+ and CD8+ T lymphocytes. In the spleen, CD3+ and CD4+ T lymphocytes were increased significantly at 30 dpi (p<0.01) whereas CD8+ and TCR γ δ+ T lymphocytes were decreased at 1 (p<0.05), 30 dpi (p<0.01). An... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell mediated immune competency; Fowl adenovirus VIII; Immune system; Peripheral blood; T lymphocyte subpopulations. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400007 Registros recuperados: 2 Primeira ... 1 ... Última

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