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| El-Nakeeb,Moustafa A; Abou-Shleib,Hamida M; Khalil,Amal M; Omar,Hoda G; El-Halfawy,Omar M. |
| Several antihistaminics possess antibacterial activity against a broad spectrum of bacteria. However, the exact mechanism of such activity was unclear. Hence, the aim of this study is to investigate their mechanism of antibacterial activity especially their effect upon the permeability of the bacterial cytoplasmic membrane. The effects of azelastine, cetirizine, cyproheptadine and diphenhydramine were studied using Gram-positive and Gram-negative multiresistant clinical isolates. Leakage of 260 and 280 nm UV-absorbing materials was detected upon treatment with the tested antihistaminics; indicative of membrane alteration. Using an artificial membrane model, cholesterol-free negatively-charged unilamellar liposomes, confirmed the effect of antihistaminics... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antihistaminics; Azelastine; Bacteria; Cytoplasmic membrane; Liposomes; Permeability alteration. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300019 |
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| Mohamed,Yasmine Fathy; Abou-Shleib,Hamida Moustafa; Khalil,Amal Mohamed; El-Guink,Nadia Mohamed; El-Nakeeb,Moustafa Ahmed. |
| Abstract Pan-drug resistant Gram-negative bacteria, being resistant to most available antibiotics, represent a huge threat to the medical community. Colistin is considered the last therapeutic option for patients in hospital settings. Thus, we were concerned in this study to demonstrate the membrane permeabilizing activity of colistin focusing on investigating its efficiency toward those pan-drug resistant isolates which represent a critical situation. We determined the killing dynamics of colistin against pan-drug resistant isolates. The permeability alteration was confirmed by different techniques as: leakage, electron microscopy and construction of an artificial membrane model; liposomes. Moreover, selectivity of colistin against microbial cells was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pan-drug resistant; Leakage; Permeability alteration; Colistin; Ultrastructure. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200381 |
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