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Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium Biol. Res.
POLANCO,RUBÉN; CANESSA,PAULO; RIVAS,ALEXIS; LARRONDO,LUIS F; LOBOS,SERGIO; VICUÑA,RAFAEL.
In this report we describe the isolation and characterization of a gene encoding the transcription factor Acel (Activation protein of cup 1 Expression) in the white rot fungus Phanerochaete chrysosporium. Pc-acel encodes a predicted protein of 633 amino acids containing the copper-fist DNA binding domain typically found in fungal transcription factors such as Acel, Macl and Haal from Saccharomyces cerevisiae. The Pc-acel gene is localized in Scaffold 5, between coordinates 220841 and 222983. A S. cerevisiae acel null mutant strain unable to grow in high-copper medium was fully complemented by transformation with the cDNA of Pc-acel. Moreover, Northern blot hybridization studies indicated that Pc-acel cDNA restores copper inducibility of the yeast cup 1...
Tipo: Journal article Palavras-chave: Acel; Basidiomycete; Copper; Phanerochaete chrysosporium.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500007
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Azo Dye Mineralization by Phanerochaete Chysosporium in a Sequencing Bath Reactor BABT
Wanderley,Carlos Ronald Pessoa; Andrade,Marcus Vinícius; Pereira,Luciana José; Silva,Gloria Maria Marinho; Pessoa,Kelly Rodrigues.
ABSTRACT The mineralization of the azo dye congo red by the fungi Phanerochaete chrysosporium was studied in two sequential batch bioreactors (R1 and R2), operated in cycles of 48 h (step I) and 24 h (step II). In step I, glucose concentration was 1 g.L-1 in both reactors and in step II, 1 g.L-1 of glucose was maintained in R1, but R2 received no addition of glucose. In step I, the average dye removal efficiencies were 76 ± 29 % (R1) and 53 ± 15% (R2), while in step II the averages recorded for dye removal for R1 and R2 were 84 ± 15 and 70 ± 28%, respectively. The rates of dye removal were 0.04 h-1 in R1 and 0.03 h-1 in R2 in step I. Higher rates were obtained in step II, 0,07 h-1 and 0,02 h-1 for R1 and R2, respectively. The highest dye removal occurred...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Congo red; Phanerochaete chrysosporium; Sequential bioreactors.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100606
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Overview of parameters influencing biomass and bioreactor performance used for extracellular ligninase production from Phanerochaete chrysosporium BABT
Ntwampe,Seteno; Chowdhury,Faysol; Sheldon,Marshall; Volschenk,Heinrich.
The production of extracellular enzymes is gaining momentum as commercial interests seek alternative ways to improve the productivity in the biotechnology and pharmaceutical industries. Early research studies looked at improving batch bioreactor operational challenges; however, the use of continuous cultures was indicated to be favourable. This led to a new approach developed to produce extracellular enzymes continuously using fixed-film bioreactors from biofilms immobilised on polymeric and inorganic membranes. In this review, the performance of P. chrysosporium biomass, evaluated in terms of ligninase production using different bioreactor operation conditions, is highlighted. Furthermore, the limitations related to the implementation of optimised batch...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phanerochaete chrysosporium; Lignin peroxidase; Manganese peroxidase; Dissolved oxygen; Bioreactors.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500008
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Enhanced production of manganese peroxidase by Phanerochaete chrysosporium BABT
Urek,Raziye Ozturk; Pazarlioglu,Nurdan Kasikara.
Production of manganese-dependent peroxidase (MnP) by the white-rot fungus Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was monitored during growth in different media and growth conditions. The effect of some activators of MnP production, Mn2+, Tween 80, phenylmethylsulphonylfloride (PMSF), oxygen, temperature, pH, glycerol and nitrogen was studied. Supplementing the cultures with Tween 80 (0.05 %, v/v) and Mn2+ (174 µM) resulted a maximum MnP activity of 356 U/L which was approximately two times higher than that obtained in the control culture (without Tween 80). Decolourisation of Direct Blue 15 and Direct Green 6 (50 mg/L) was also achieved with MnP.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phanerochaete chrysosporium; Manganese dependent peroxidase; Tween 80; Mn2+; Phenylmethylsulphonylfloride.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700001
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Effect of single cell protein as a protein source in Drosophila culture BJM
Uysal,Handan; Aydogan,M. Nuri; Algur,O. Faruk.
The effects of biomass of Phanerochaete chrysosporium at various concentrations (1; 2.5; 5; 10; 25; 50; 75 and 100%) on the growth of Drosophila melanogaster have been investigated. Biomass was used as a protein source instead of corn flour in Standard Drosophila Medium(SDM). It was seen that it causes increments in both body size of larvae and the numbers of offsprings, especially at higher concentrations (50, 75 and 100%). At the application of 100% concentration, metamorphosis accelerated and was completed one day before the control. There were phenotipic abnormalities in both control (0.64%) and applications with low concentrations (0.02-0.19%), while they were not observed at the application of 100% concentration
Tipo: Info:eu-repo/semantics/article Palavras-chave: Single cell protein; Toxicity; Drosophila; Phanerochaete chrysosporium.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400007
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