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| Emeline, Creis Bendelac; Ludovic, Delage; Laurent, Vallet; Catherine, Leblanc; Kruse, Inken; Erwan, Ar Gall; Florian, Weinberger; Philippe, Potin. |
| Mechanisms related to the induction of phlorotannin biosynthesis in marine brown algae remain poorly known. Several studies undertaken on fucoid species have shown that phlorotannins accumulate in the algae for several days or weeks after being exposed to grazing, and this is measured by direct quantification of soluble phenolic compounds. In order to investigate earlier inducible responses involved in phlorotannin metabolism, Fucus vesiculosus was studied between 6 and 72 h of grazing by the sea snail Littorina littorea. In this study, the quantification of soluble phenolic compounds was complemented by a Quantitative real-time PCR (qRT-PCR) approach applied on genes that are potentially involved in either the phlorotannin metabolism or stress responses.... |
| Tipo: Text |
Palavras-chave: Phlorotannins; Inducible defense; Gene expression; Grazing; Fucus vesiculosus. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00688/79981/82940.pdf |
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| Vandanjon, Laurent; Vallet, Laurent; Le Glatin, Titouan; Deleris, Paul; Baron, Regis; Bourseau, Patrick; Dumay, Justine. |
| The use of surfactants to improve enzymatic hydrolysis of the macroalgae Sargassum muticum has been investigated. Visible absorption spectroscopy has been used to quantify the solubilization of both polysaccharides and phlorotannins in the hydrolysates. After total extraction, results showed that Sargassum muticum contained 2.74% (expressed in percent of the dry weight of the algae) of phlorotannins whose 32 % were in the cell wall. This result shows that it is important to access to the parietal phlorotannins. To reach this objective, we chose the enzymatic approach for destructurating the cell wall of the algae. The use of 5% dry weight (DW - 5% by weight of hydrolyzed algae) of an enzymatic mix containing a commercial beta-glucanase, a commercial... |
| Tipo: Text |
Palavras-chave: Brown macroalgae; Sargassum muticum; Enzymatic hydrolysis; Surfactants; Phlorotannins. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00350/46105/46073.pdf |
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