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	Provedor de dados OAK (4) Autor Hashimoto, Makoto (3) Hatanaka, Yasumaru (3) 橋本, 誠 (2) HASHIMOTO, Makoto (1) HATANAKA, Yasumaru (1) KATO, Yuh-hi (1) Nabeta, Kensuke (1) Okamoto, Shun’ji (1) Mais... Palavra-chave Diazirine (4) Photoaffinity labeling (4) Avidin-biotin (1) Bioorganic chemistry (1) Biotin (1) Chemical biology (1) Chymotrypsin (1) Hydrogenation (1) Isoelectric focusing (1) Structure-activity relationships (1) V8 protease (1) Wilkinson’s catalyst (1) Mais... Ano 2008 (1) 2007 (1) 2006 (1) 2004 (1) País Japan (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Positively coded photoaffinity label for altering isoelectric points of proteins OAK Hashimoto, Makoto; Hatanaka, Yasumaru. Novel diazirinyl photoaffinity ligand, which contains (3-trifluoromethyl) phenyldiazirine and penta(epsilon-Boc-Lys) as a photoreactive code, allows the introduction of a positive cascade to alter the pI value of labeled components, facilitating the isolation of photolabeled biocomponents with isoelectric focusing techniques. (c) 2006 Elsevier Ltd. All rights reserved. Palavras-chave: Photoaffinity labeling; Diazirine; Isoelectric focusing; Biotin. Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1321 Recent progress in diazirine based photoaffinity labeling OAK Hashimoto, Makoto; Hatanaka, Yasumaru; 橋本, 誠. Based on the recent development of molecular biology, the investigation of biofunctional machinery at their ligand accepting interface has become one of the challenging and important subject of post-genome field. The technique of photoaffinity labeling has become increasingly appreciated as a powerful methodology for the purpose. This micro review focused synthesis of trifluoromethylphenyldiazirine, which is one of the most promising photophore, and its application to the biomolecules. Palavras-chave: Photoaffinity labeling; Chemical biology; Bioorganic chemistry; Structure-activity relationships; Diazirine. Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2700 Selective Hydrogenation of Alkene in (3-Trifluoromethyl) Phenyldiazirine Photophor with Wilkinson’s Catalyst for Photoaffinity Labeling OAK HASHIMOTO, Makoto; KATO, Yuh-hi; HATANAKA, Yasumaru; 橋本, 誠. Selective hydrogenation of carbon–carbon double bond in the presence of nitrogen–nitrogen double bond in (3-trifluoromethyl) phenyldiazirine achieved with Wilkinson's catalyst. Palavras-chave: Photoaffinity labeling; Diazirine; Hydrogenation; Wilkinson’s catalyst. Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3611 Enzyme cleavable and biotinylated photoaffinity ligand with diazirine OAK Hashimoto, Makoto; Okamoto, Shun’ji; Nabeta, Kensuke; Hatanaka, Yasumaru. The efficient synthesis of an enzyme cleavable biotinylated diazirinyl photoaffinity ligand is described to allow the effective manipulation of the photolabeled biocomponents. The compound contains a glutamic acid gamma-methyl ester, which is a precursor of the substrate for V8 protease, between the diazirinyl photophor and biotin moiety. After alkaline hydrolysis of the ester, the compound can be proteolyzed at the Glu moiety by V8 protease. The photophore was introduced to L-Phe p-nitroanilide and the ligand was applied to photolabel of chymotrypsin to manipulate the application of the concept. Palavras-chave: Photoaffinity labeling; Diazirine; Avidin-biotin; V8 protease; Chymotrypsin. Ano: 2004 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/738 Registros recuperados: 4 Primeira ... 1 ... Última

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