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Morphological and physiological photon flux influence under in vitro culture of apple shoots BABT
Zanandrea,Ilisandra; Bacarin,Marcos Antonio; Braga,Eugenia Jacira Bolacel; Bianchi,Valmor João; Peters,José Antonio.
The aim of the present study was to evaluate the growth and development of apple rootstock shoots submitted to different photon flux densities (7, 14, 21 and 60 µmol m-2 s-1) and three culture media. The best photon flux for maximizing growth, number of shoots and leaves was 14 µmol m-2 s-1, which also resulted in the highest values of chloroplastic pigments. On the other hand, the highest photon flux (60 µmol m-2 s-1) decreased significantly these parameters. Medium with reduced sucrose concentration did not affect the analyzed parameters, enabling an efficient multiplication of the cultivar with only 1% of sucrose.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mallus; Apple; Sucrose; Photon flux density; Culture medium.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500005
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Chlorophyll a fluorescence of sweet potato plants cultivated in vitro and during ex vitro acclimatization Braz. J. Plant Physiol.
Cassana,Francine F.; Falqueto,Antelmo R.; Braga,Eugenia J. B.; Peters,Jose A.; Bacarin,Marcos A..
Sweet potato (Ipomoea batatas L.) plants were cultivated in vitro in Murashige and Skoog (MS) medium with 20 and 40 g L-1 of sucrose under two different photon flux densities (21 and 60 µmol m-2 s-1). Leaves developed in vitro mostly showed high variable to maximum fluorescence ratio (F V/F M) , indicating good development of photosynthetic apparatus. This ratio decreased during leaf aging, especially in the plants cultivated in vitro on medium with higher sucrose concentration and higher photon flux. Leaves developed ex vitro showed high F V/F M ratio during whole experiment. The effective photochemical efficiency (F V'/F M') was maximum at 15th day after emergence of leaves. Photosynthetic potential rate was higher in leaves developed in vitro than in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Culture medium; Photon flux density; Photosystem; Sucrose.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000300003
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