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| GAWADE,S. P.. |
| The photodynamic action of UV sensitized methylene blue (MB) was studied on the venom of Thailand cobra Naja siamensis. The reaction mixture comprising phosphate buffer (B), methylene blue (S), and venom protein (P) was exposed to UVR (ultra violet radiation) for 2 hours at 37°C. Stable excited species (S) were generated by photodynamic action. These in B environment produced reduced substates SH2 and photooxidised products PO in the presence of atmospheric oxygen. Increased absorbance (OD) of the reaction mixture after UVR with a corresponding loss of protein was suggested to be due to the formation of photooxidised venom products. The detoxification profile of venom protein using visible radiation in the presence of MB and oxygen appeared similar to the... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Thailand cobra; Naja siamensis; Photosensitization; Photooxidation; Detoxification. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302000000200010 |
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| Kiyota, Maki; Numayama, Naoko; Goto, Ken. |
| Plant defenses against photo-oxidative stress have been studied almost exclusively with respect to stress responses, and little is known about how non-enzymic antioxidants change under constant conditions without a time cue or an environmental stress. Here, we show that, in both the flagellated alga Euglena gracilis Z and the angiosperm Spinacia oleracea L., the potent antioxidant L-ascorbic acid (Asc) displays a circadian rhythm with a maximum at subjective midday, a physiological state reflecting that attained at noon under daily light/dark cycles. Thus, photosynthetic organisms can maximize antioxidant levels in anticipation of midday, when photo-oxidative stress is most severe. These results may partly explain the in-phase circadian UV-C resistance... |
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Palavras-chave: Antioxidant; L-ascorbic acid; Circadian; Euglena gracilis; Midday; Photooxidation; Spinach. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/716 |
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