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	Provedor de dados BJM (1) Autor Couri,Sonia (1) Greiner,Ralf (1) Silva,Lucineia Gomes da (1) Palavra-chave Aspergillus niger (1) Phytase (1) Phytate (1) Phytate-degrading enzyme (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2009 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Purification and characterisation of an extracellular phytase from Aspergillus niger 11T53A9 BJM Greiner,Ralf; Silva,Lucineia Gomes da; Couri,Sonia. An extracellular phytase from Aspergillus niger 11T53A9 was purified about 51-fold to apparent homogeneity with a recovery of 20.3% referred to the phytase activity in the crude extract. Purification was achieved by ammonium sulphate precipitation, ion chromataography and gel filtration. The purified enzyme behaved as a monomeric protein with a molecular mass of about 85 kDa and exhibited maximal phytate-degrading activity at pH 5.0. Optimum temperature for the degradation of phytate was 55°C. The kinetic parameters for the hydrolysis of sodium phytate were determined to be K M = 54 µmol l-1 and k cat = 190 sec-1 at pH 5.0 and 37°C. The purified enzyme was rather specific for phytate dephosphorylation. It was shown that the phytase preferably... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger; Phytate-degrading enzyme; Phytate; Phytase. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400010 Registros recuperados: 1 Primeira ... 1 ... Última

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