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Acquisition de données complémentaires aux dénombrements, avec les techniques d’analyses pigmentaires et de diversité génétique. Etat d’avancement et premiers résultats. Action Indice Composition. Livrable n° AIV. Rapport final, 16 juillet 2014. ArchiMer
Delmas, Daniel; Siano, Raffaele.
Studies on phytoplankton community diversity cannot be limited microphytoplankton (cells>20 ìm), it is indeed necessary to take into account also the nano (3-20ìm) picophytoplankton (<3 ìm) components. In the frame of this work aimed at studying temporal phytoplankton community diversity two complementary methodologies of phytoplankton sample analysis, pigment and genetic (metarbarcoding) diversity, have been used as alternative to routine morphological analysis carried out at light microscopy. The REPHY station (047-P-016 Concarneau Large) off-shore the Concarneau Bay has been sampled during the annual highest phytoplankton production (March-July 2012) with a frequent sample interval strategy (twice per week). Results obtained by both pigment and...
Tipo: Text Palavras-chave: Phytoplancton; Structure de taille; Picophytoplancton; Nanophytoplancton; Microphytoplancton; Diversité génétique; Metabarcoding; Taxonomie pigmentaire; Façade atlantique; Baie de Concarneau; Phytoplankton; Size classes; Picophytoplankton; Nanophytoplankton; Microphytoplankton; Genetic diversity; Metabarcoding; Pigment taxonomy; Atlantic coasts; Concarneau Bay.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00361/47260/47225.pdf
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Définition d’un indice composition pour le phytoplancton en Manche- Atlantique, à partir des données du micro-phytoplancton du REPHY et des réseaux régionaux, et des mesures complémentaires de la flore phytoplanctonique acquises avec des techniques novatrices. Action 3 – Livrable 1. Propositions pour un indice de composition du phytoplancton, basé sur les résultats des méthodes microscopie, pigments et diversité génétique. Rapport final, février 2016 ArchiMer
Hernandez Farinas, Tania; Brun, Melanie; Siano, Raffaele; Delmas, Daniel.
This deliverable is composed of two reports which propose indice of phytoplankton composition, based respectively on the results of pigment and genetic diversity methods, and the results of microscopy method. Proposals based on the results of pigment and genetic diversity methods. The present work is a continuation of several studies carried out in the past (Siano and Delmas, 2013, 2015) on investigating an indicator of phytoplankton diversity using two methods complementary to optical microscopy: the genetic diversity analyzed with metabarcoding (exhaustive analysis of the diversity and relative abundance of OTUs, i.e. groups of sequences identified by their genetic resemblance) and the pigmentary diversity (analysis of the diversity and abundance of...
Tipo: Text Palavras-chave: Metabarcoding; OTUs; Pigments; Picophytoplancton; Indice composition; Niche ecologique; Mamiellophyceae; Chlorophylle b; Rade de Brest; Bretagne sud; Panache de la Gironde; Denombrements du micro-phytoplancton; Abondance totale; Monotonicite; Linearite; Pressions; Facade Manche-Atlantique; Metabarcoding; OTUs; Pigments; Picophytoplankton; Composition indice; Ecological niche; Mamiellophyceae; Chlorophyll b; Brest Bay; Southern Britain; Gironde plume; Micro-phytoplankton counts; Total abundance; Monotonicity; Linearity; Pressure; Channel-Atlantic coast.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00363/47421/47426.pdf
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