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| Renault, Tristan. |
| Mortalities in a number of commercially important mollusc species have been associated with the detection of viruses belonging to several families. The first description of a virus was in adult eastern oyters, Crassostrea virginica, with the detection of virus particles resembling members of the family Herpesviridae. Subsequently, mass mortalities in French stocks of adult Portuguese oysters, C. angulata, were associated with irido-like virus infection. Other viruses observed in molluscs are described as members of the families Iridoviridae, Papovaviridae and Reoviridae. Little information is available on viral infections that affect molluscs due to primarily to the inadequacy of diagnostic methods that are employed when mass mortality events occur. Most... |
| Tipo: Text |
Palavras-chave: Virus; Scallops; Picornavirus; Oyter herpesvirus 1; Oysters; Molluscs; Irido Like virus; Diseases; Herpesvirus; Clams; Bivalves; Birnavirus; Abalones. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4932.pdf |
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| Mari, Jocelyne; Bonami, Jean-robert; Lightner, Donald V. |
| The ssRNA genome extracted from purified Taura Syndrome Virus (TSV) was transcribed into double-stranded, blunt-ended cDNA and was used to construct cDNA libraries either in pUC 18 or in pBluescript II KS-vectors. Twelve recombinant plasmids chosen after screening of the libraries were subjected to restriction enzyme digestions for determination of size inserts and restriction maps. Two of them, pP15 and pQ1, were selected for probe construction. The inserts, 1500 and 1300 base pairs (bp) respectively, were DIG-11dUTP-labelled and the corresponding probes were named P15 and Q1. On northern blots and dot blots, using different denaturation methods, the 2 probes hybridized specifically with extracted RNA-TSV genome, TSV and infected TS shrimp homogenates. No... |
| Tipo: Text |
Palavras-chave: TSV; Penaeid shrimp; Picornavirus; Cloning; Dot blot hybridization; In situ hybridization. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00447/55899/57914.pdf |
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