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	Provedor de dados BABT (1) BJM (1) Autor Adnan,Ahmad (1) Ahmad,Waqar (1) Das,Debasish (1) Goyal,Arun (1) Jamil,Sania (1) Kothari,Damini (1) Noor,Bushra (1) Patel,Seema (1) Rafique,H.M. (1) Shabbiri,Khadija (1) Shukla,Rishikesh (1) Mais... Palavra-chave Plackett-Burman Design (2) Brevibacterium linens (1) Central Composite Design (1) Central Composite Design (CCD) (1) Dextran (1) Pediococcus pentosaceus (1) Protease (1) Response Surface Methodology (1) Solid-state fermentation (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2012 (1) 2011 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Scale up of dextran production from a mutant of Pediococcus pentosaceus (SPAm) using optimized medium in a bioreactor BABT Patel,Seema; Kothari,Damini; Shukla,Rishikesh; Das,Debasish; Goyal,Arun. The mutant of Pediococcus pentosaceus (SPAm) produced earlier by UV-mutagenesis exhibiting higher dextransucrase activity as compared to wild-type was used. The generated mutant SPAm gave 12.2 mg/ml, a 20% higher dextran than wild-type. Response surface methodology was carried out for further enhancement of dextran production. To enhance dextran production by the mutant SPAm, Plackett-Burman Design and a 2² full factorial Central Composite Design was employed. After response optimization, the optimum concentration of sucrose and yeast extract was 5.115% (w/v) and 0.635% (w/v), respectively. The experimental values of dextran 36.0 mg/ml at flask level and 35.0 mg/ml at bioreactor level were in good agreement with the predicted value of 40.8 mg/ml. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pediococcus pentosaceus; Dextran; Plackett-Burman Design; Central Composite Design; Response Surface Methodology. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600007 Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach BJM Shabbiri,Khadija; Adnan,Ahmad; Jamil,Sania; Ahmad,Waqar; Noor,Bushra; Rafique,H.M.. Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett-Burman design and four significant variables (soybean meal, wheat bran, (NH4)2SO4 and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH4)2SO4) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium. Tipo: Info:eu-repo/semantics/article Palavras-chave: Brevibacterium linens; Protease; Solid-state fermentation; Plackett-Burman Design; Central Composite Design (CCD). Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300031 Registros recuperados: 2 Primeira ... 1 ... Última

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