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| Ribeiro, Roana Cecília dos Santos; Ribeiro, Thaís Rafaelle dos Santos; Souza-Motta, Cristina Maria de; Medeiros, Erika Valente; Moreira, Keila Aparecida. |
| The current study evaluated the proteases production from 11 fungal species belonging to the genera Mucor, Rhizomucor and Absidia. The species were obtained from the Collection of Cultures URM at the Mycology Department-UFPE, Brazil. The best producing species was Mucor hiemalis URM 3773 (1.689 U mL-1). Plackett-Burman design methodology was employed to select the most effective parameter for protease production out of 11 medium components, including: concentration of filtrate soybean, glucose, incubation period, yeast extract, tryptone, pH, aeration, rotation, NH4Cl, MgSO4 and K2HPO4. Filtrated soybean concentration was the significant variable over the response variable, which was the specific protease activity. The crude enzyme extract showed optimal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2.12.00.00-9; 2.12.02.00-1; 2.12.02.02-8 proteolytic activity; Substrate fermentation liquid; Plackett-Burman design; Biochemical characterization Microbiologia Industrial e de Fermentação. |
| Ano: 2015 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/21016 |
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| Coghetto,Chaline Caren; Vasconcelos,Carolina Bettker; Brinques,Graziela Brusch; Ayub,Marco Antônio Záchia. |
| Abstract In this study, physiological aspects of Lactobacillus plantarum BL011 growing in a new, all-animal free medium in bioreactors were evaluated aiming at the production of this important lactic acid bacterium. Cultivations were performed in submerged batch bioreactors using the Plackett-Burman methodology to evaluate the influence of temperature, aeration rate and stirring speed as well as the concentrations of liquid acid protein residue of soybean, soy peptone, corn steep liquor, and raw yeast extract. The results showed that all variables, except for corn steep liquor, significantly influenced biomass production. The best condition was applied to bioreactor cultures, which produced a maximal biomass of 17.87 g L-1, whereas lactic acid, the most... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lactobacillus plantarum BL011; Lactic acid; Liquid acid protein residue of soybean; Plackett-Burman design; Industrial production of biomass. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400941 |
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| Yu,Ying; Zhou,Xiaoyu; Wu,Sheng; Wei,Tiantian; Yu,Long. |
| Background Lysozyme plays a crucial role in innate immunity with its well-recognized bacteriolytic activity. In this study, the influence of expression parameters (inoculation volume, culture volume, growth time, induction temperature and time, initial pH and methanol concentration) on human lysozyme (HLZ) production in recombinant P. pastoris SMD1168 was investigated through Plackett-Burman (PB) design and response surface methodology (RSM). Results It was revealed that induction temperature, induction time and culture volume had significant influence (P < 0.01) on HLZ expression level, which were elected for further optimization with three-dimensional response surface designs for enhanced HLZ production. The highest lysozyme activity reached 3301 U/mL... |
| Tipo: Journal article |
Palavras-chave: Expression optimization; LYZ; Plackett-Burman design; RSM. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600009 |
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