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| Nunes,Claudinéia Ferreira; Ferreira,Juliano Lino; Fernandes,Mônique Carolina Nunes; Breves,Sâmera de Souza; Generoso,Andressa Leal; Soares,Bárbara Dantas Fontes; Dias,Mário Sérgio Carvalho; Pasqual,Moacir; Borem,Aluízio; Cançado,Geraldo Magela de Almeida. |
| Several extraction methods of genomic DNA for identification and characterization of genetic diversity in different plant species are routinely applied during molecular analysis. However, the presence of undesirable compounds such as polyphenols and polysaccharides is one of the biggest problems faced during the isolation and purification of high quality DNA in plants. Therefore, achievement of fast and accurate methods for DNA extraction is crucial in order to produce pure samples. Leaves of strawberry genotypes (Fragaria ananassa) have high contents of polysaccharides and polyphenols which increase the sample viscosity and decrease the DNA quality, interfering with the PCR performance. Thereby, in this study we evaluated the quality and amount of genomic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fragaria ananassa; Genomic DNA; Polivinilpirrolidone; Lyophilization; PCR. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000800014 |
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