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Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis J. Venom. Anim. Toxins incl. Trop. Dis.
Diniz-Sousa,Rafaela; Kayano,Anderson M.; Caldeira,Cleópatra A.; Simões-Silva,Rodrigo; Monteiro,Marta C.; Moreira-Dill,Leandro S.; Grabner,Fernando P.; Calderon,Leonardo A.; Zuliani,Juliana P.; Stábeli,Rodrigo G.; Soares,Andreimar M..
Abstract Background: Wasp venoms constitute a molecular reservoir of new pharmacological substances such as peptides and proteins, biological property holders, many of which are yet to be identified. Exploring these sources may lead to the discovery of molecules hitherto unknown. This study describes, for the first time in hymenopteran venoms, the identification of an enzymatically inactive phospholipase A2 (PLA2) from the venom of the social wasp Polybia occidentalis. Methods: P. occidentalis venom was fractioned by molecular exclusion and reverse phase chromatography. For the biochemical characterization of the protein, 1D and 2D SDS-PAGE were performed, along with phospholipase activity assays on synthetic substrates, MALDI-TOF mass spectrometry and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wasp; Polybia occidentalis; PocTX; PLA2 homologue.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100302
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Promotion of thermoregulatory insulation in nests of neotropical wasps by building extra-combs with empty cells Neotropical Entomology
Hozumi,Satoshi; Kudô,Kazuyuki; Zucchi,Ronaldo.
We examined the hypotheses that the empty combs of Polybia occidentalis Olivier (Hymenoptera: Vespidae) nest insulate the inside of the nest. To examine this hypotheses, two kinds of temperature measurements were carried out: 1) with the outer comb of the nest intact (Control) and 2) removed (Treatment), using a large and a small nest. In the large nest, the daily nest temperatures (outer part, Tn1; inner part, Tn2) in Control were lower by 0.6°C (Tn1) and 1.2°C (Tn2) than those in Treatment, because of a higher ambient temperature (Ta) throughout temperature assessment in Treatment. However, the excess temperature (Tn - Ta) in Control was higher than that in Treatment. The value was higher by 0.7°C at Tn1 and 0.1°C at Tn2. In the small nest, the excess...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polistinae; Polybia occidentalis; Nest architecture; Thermoregulation; Winter in neotropics.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2008000200008
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