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| Registros recuperados: 30 | |
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| Ribon,Andréa de O.B.; Ribeiro,João Batista; Gonçalves,Daniel B.; Queiroz,Marisa V. de; Araújo,Elza F. de. |
| Previous reports have described pgg2, a polygalacturonase-encoding gene of Penicillium griseoroseum, as an attractive model for transcriptional regulation studies, due to its high expression throughout several in vitro growth conditions, even in the presence of non-inducing sugars such as sucrose. A search for regulatory motifs in the 5' upstream regulatory sequence of pgg2 identified a putative CCAAT box that could justify this expression profile. This element, located 270 bp upstream of the translational start codon, was tested as binding target for regulatory proteins. Analysis of a 170 bp promoter fragment by electrophoretic mobility shift assay (EMSA) with nuclear extracts prepared from mycelia grown in pectin-containing culture medium revealed a high... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Penicillium griseoroseum; Polygalacturonase; 5' upstream regulatory sequence; Electrophoretic mobility shift assay. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100019 |
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| 高橋, 裕司; 井熊, 武志; 匂坂, 慶子; 斉藤, 泉; 髙澤, 俊英; TAKAHASHI, Yuji; IKUMA, Takeshi; SAGISAKA, Keiko; SAITO, Izumi; TAKASAWA, Toshihide. |
| CM-Toyopearl陽イオン交換カラムクロマトグラフィーおよびSephacryl S-200ゲルろ過カラ ムクロマトグラフィーによって、常温性菌核病菌Sclerotinia sclerotiorumの20℃培養抽出物 のポリガラクツロナーゼ活性画分からポリガラクツロナーゼⅠを単離した。ポリガラクツ ロナーゼⅠ精製標品(P4-4-2)の比活性は3 662U/mgであり、粗抽出液から全体として116倍に 精製した。精製標品はポリアクリルアミドゲル電気泳動によって、均一であることが示さ れた。また、SDS存在下ポリアクリルアミドゲル電気泳動によって本酵素の分子質量は 39.8±1.10kDa(S.E.)、さらに等電点電気泳動によって本酵素の等電点はpH8.70と見積もられた。 Polygaracturonasehas been isolated from the mesophilic white mold Sclerotinia Sclerotiorum by cation-exchange chromatography on a column of CM-Toyopearl followed by gel filtration on a column of Sephacryl S-200. Purified enzyme preparation(P4-4-2) had a specific activity of 3 662 units per mg of protein, that is, 116-fold purification over the crude extract of the wheat bran culture of the mesophilic fungus S.... |
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Palavras-chave: Sclerotinia sclerotiorum; 常温菌; 菌核病菌; ポリガラクツロナーゼ; 細胞壁分解酵素; Mesophile; White mold; Polygalacturonase; Cell wall degrading enzyme. |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/106 |
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| 渡辺, 剛志; 波川, 啓土; 斉藤, 泉; 髙澤, 俊英; WATANABE, Tsuyoshi; NAMIKAWA, Yoshitada; SAITO, Izumi; TAKASAWA, Toshihide. |
| 低温性通性雪腐菌核病菌Sclerotinia trifoliorum の低温適応現象を解明するために, 5℃及び20℃で産生するポリガラクツロナーゼ(PGase) 活性の性質を調べた｡ S. trifoliorum のアルファルファ培地での培養は5℃では160日間(最適培養日数), 20℃では45日間(最適培養日数) 行った｡ 5℃及び20℃培養粗抽出液のPGase 活性は, それぞれ30.8±0.4 U/mL (抽出体積462mL, Total activity 14 200U), 36.5±0.9 U/mL (抽出体積454mL, Total activity 16 600U) であり, 低温 においてもPGase 産生量が著しく低下しないことがわかった｡これら粗抽出液をBio-Gel P-6 DG ゲル濾過クロマトグラフィーによる脱塩操作後に, 5℃及び20℃培養脱塩画分のPGase 活性の性質を調べた｡温度依存性については, 最適温度は5℃及び20℃培養PGase 活性ともに50℃であり, 50℃での活性に対する各温度での相対活性によって両者を較べると, それらの曲線は両者でほぼ一致した｡温度安定性については, 残存活性で比較すると, 5℃培養PGase 活性は15℃～35℃において僅かであるが20℃培養のものにくらべて不安定性を示した｡一方, 45～55℃では若干の安定性を示した｡pH 依存性については, ５℃培養PGase 活性の最適pH は4.5であり, 20℃培養は4.0であった｡従って, これらの結果は5℃及び20℃培養によって産生された各々のPGase アイソザイムが異なることを示した｡ In order to clarify cold adaptation phenomenon of... |
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Palavras-chave: Sclerotinia trifoliorum; 低温菌; ポリガラクツロナーゼ; 低温適応; 細胞壁分解酵素; Sclerotinia trifoliorum; Psychrotroph; Polygalacturonase; Cold adaptaition; Cell wall degrading enzyme. |
| Ano: 2004 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/102 |
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| 高橋, 裕司; 井熊, 武志; 匂坂, 慶子; 斉藤, 泉; 髙澤, 俊英; TAKAHASHI, Yuji; IKUMA, Takeshi; SAGISAKA, Keiko; SAITO, Izumi; TAKASAWA, Toshihide. |
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Palavras-chave: Sclerotinia borealis; 好冷菌; 雪腐菌核病菌; ポリガラクツロナーゼ; 細胞壁分解酵素; Psychrophile; Snow mold; Cell wall degrading enzyme; Polygalacturonase. |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/120 |
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| 竹内, 宏治; 井熊, 武志; 匂坂, 慶子; 斉藤, 泉; 髙澤, 俊英; TAKEUCHI, Kouji; IKUMA, Takeshi; SAGISAKA, Keiko; SAITO, Izumi; TAKASAWA, Toshihide. |
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Palavras-chave: ポリガラクツロナーゼ; 低温適応; 好冷菌; Sclerotinia borealis; Sclerotinia sclerotiorum; Polygalacturonase; Cold adaptation; Psychrophile. |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/121 |
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| RUIZ,GLORIA ANNABELL COBEÑA; SALOMÃO,LUIZ CARLOS CHAMHUM; SIQUEIRA,DALMO LOPES DE; REZENDE,SEBASTIÃO TAVARES DE; LINS,LEILA CRISTINA ROSA DE. |
| ABSTRACT A major problem in post-harvest handling of bananas is the individual detachment of the fruit from the hands. This study aimed to establishing the relationship between carbohydrate concentration and enzyme activity in the pedicel region of three cultivars of bananas, resistant and susceptible to natural dropping, during post-harvest ripening, and the susceptibility of bananas to finger dropping. Cultivars ‘Terra’ (plantain, AAB group) and ‘Prata’ (banana, AAB group) triploids and the ‘Prata Graúda’ (banana, AAAB group) tetraploid were used. The experiment was distributed in split plots, with three plots (cultivars) and five subplots (peel color stages) in a completely randomized design with three replications and three fruits per sample unit.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Musa sp.; Pectinametylesterase; Polygalacturonase; Starch. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452016000300301 |
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| Soares,Márcia M.C.N.; Silva,Roberto da; Gomes,Eleni. |
| One hundred sixty eight bacterial strains, isolated from soil and samples of vegetable in decomposition, were screened for the use of citrus pectin as the sole carbon source. 102 were positive for pectinase depolymerization in assay plates as evidenced by clear hydrolization halos. Among them, 30% presented considerable pectinolytic activity. The cultivation of these strains by submerged and semi-solid fermentation for polygalacturonase production indicated that five strains of Bacillus sp produced high quantities of the enzyme. The physico-chemical characteristics, such as optimum pH of 6.0 - 7.0, optimum temperatures between 45oC and 55oC, stability at temperatures above 40oC and in neutral and alkaline pH, were determined. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus sp; Polygalacturonase; Pectinolytic activity. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400002 |
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| Raupp, Dorivaldo da Silva; UEPG; Porfírio-da-Silva, Luciane Curtes; Instituo Paranaense de Assistência Técnica e Extensão Rural (EMATER); Almeida, Mareci Mendes de; UEPG; Borsato, Aurélio Vinicius; Embrapa Pantanal. |
| Considerando a importância comercial do caqui ‘Fuyu’, a pesquisa teve como objetivos avaliar o efeito da promalina aplicada durante o cultivo do caqui ‘Fuyu’ bem como o uso de diferentes temperaturas de armazenamento na qualidade pós-colheita dos frutos. A utilização da câmara fria (4ºC, uso comercial) manteve a boa firmeza dos frutos para a comercialização acima de 16 dias, para os frutos, sem e com a aplicação de promalina no pomar, enquanto que, na temperatura ambiente (20-26ºC), os tempos foram de 16 e oito dias, respectivamente. A promalina contribuiu para a melhoria da firmeza dos frutos armazenados em câmara fria, mas não produziu o mesmo efeito em frutos armazenados em temperatura ambiente. A atividade da enzima poligalacturonase (PG) foi... |
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Palavras-chave: 5.01.00.00 - 9; 5.01.03.00 - 8; 5.07.03.02 - 1 Diospyrus kaki; Armazenamento refrigerado; Firmeza; Poligalacturonase Agronomia; Pós-colheita e armazenamento de alimentos vegetais Diospyrus kaki; Refrigerated storage; Firmness; Polygalacturonase. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6015 |
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| Becker, Fernanda Salamoni; Vilas Boas, Ana Carolina; Sales, Adones; Tavares, Lucas Silveira; Siqueira, Heloisa Helena de; Vilas Boas, Eduardo Valério de Barros. |
| This work aimed to evaluate the physicochemical characteristics and enzymatic activity of “Sabará” jabuticaba fruits, which were cultivated in Lavras, Minas Gerais State, Brazil, at different maturation stages. The fruits were harvested and separated into five different stages: totally green fruit, fruit with incipient pigmentation, fruit with a light purple predominance, totally purple fruit and totally dark purple fruit. These fruits were physicochemically analyzed and evaluated for polygalacturonase enzymatic activity. Chlorophyll degradation and the synthesis of anthocyanin on fruit rinds were observed, as well as the degradation of the cell wall and its consequent softening. In the pulp, increased soluble solids, pH and sugars were observed, while... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 50700006 CIÊNCIA E TECNOLOGIA DE ALIMENTOS 50701045 FISIOLOGIA PÓS-COLHEITA Myrciaria jaboticaba; Pectin; Polygalacturonase; Chlorophyll; Anthocyanin. FISIOLOGIA PÓS-COLHEITA. |
| Ano: 2015 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19499 |
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| LOCATELLI,GABRIEL OLIVO; FINKLER,LEANDRO; FINKLER,CHRISTINE L.L.. |
| Abstract: The present work investigated what the appropriate methods of hydrolysis of pectin for reducing compounds (RCs) production, employed as a substrate for cell growth of Cupriavidus necator. This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%). It was analyzed as a variable response for both experimental design, the RCs’ production. The best conditions of each procedure were used... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cupriavidus necator; Galacturonic acid; Pectin depolymerization; Pectin hydrolysates; Polygalacturonase. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300605 |
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| Damásio,André Ricardo de Lima; Maller,Alexandre; Silva,Tony Márcio da; Jorge,João Atílio; Terenzi,Hector Francisco; Polizeli,Maria de Lourdes Teixeira de Moraes. |
| Fungi collected from Brazilian soil and decomposing plants were screened for pectinase production. R. microsporus var. rhizopodiformis was the best producer and was selected to evaluate the pectic enzyme production under several nutritional and environmental conditions. The pectinase production was studied at 40ºC, under 28 carbon sources-supplemented medium. The inducer effect of several agro-industrial residues such as sugar cane bagasse, wheat flour and corncob on polygalacturonase (PG) activity was 4-, 3- and 2-fold higher than the control (pectin). In glucose-medium, a constitutive pectin lyase (PL) activity was detected. The results demonstrated that R. microsporus produced high levels of PG (57.7 U/mg) and PL (88.6 U/mg) in lemon peel-medium. PG had... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agro industrial residues; Lemon peel; Pectin lyase; Polygalacturonase; R. microsporus var. rhizopodiformis. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100019 |
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| Martos,María A.; Martinez Vazquez,Francisco; Benassi,Fernando O.; Hours,Roque A.. |
| Response surface methodology was used for optimization of polygalacturonase (PG) and pectinesterase (PE) production in submerged fermentation by A.niger. A Central Composite Experimental Design was applied, consisting of 22 experiments, including eight central points. Variables studied were: fermentation time (24 to 120 h), pH (3.5 to 6.5) and initial concentration of pectin (5 to 20 g/l). Maximum PE production was 220 U/l, after 74 h of culture, in a medium containing 20 g/l of pectin (pH 6.5). The optimal conditions for PG production were pH: 4.1, 20 g/l of pectin and 94 h of fermentation with a maximum value of 1032 U/l. Under these conditions, the PE production was low (15 U/l). A liquid extract with high PG activity and low PE activity could be... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pectinases; Polygalacturonase; Pectinesterase; Aspergillus niger; Response surface methodology; Citrus pectin. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300007 |
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| Registros recuperados: 30 | |
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