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Laha, R; Sasmal, N.K; Bandyopadhyay, S. |
Trypanosoma evansi from buffalo, cattle and horse isolates were purified by Diethylaminoethyl cellulose column chromatography. The purified trypanosomes from each sources were used separately for preparation of whole cell lysate antigens. A comparative study on polypeptide pattern of whole cell lysate antigens of these three isolates of T. evansi was studied. It showed a total of 11 dominant polypeptide bands with relative molecular weight ranging from 95 to 13 kDa, when resolved in 10% sodium dodecyl sulphate polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue. Except some minor differences between the relative molecular weight of isolates in a particular region, the polypeptide profiles of these three isolates did not resolve any... |
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Palavras-chave: Buffalo; Cattle; Horse; Polypeptide profiles; Trypanosoma evansi. |
Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1833 |