| The objective of present study was to examine the effect of molasses and ammonium sulfate concentration on intracellular lipid accumulation by Trichosporon asahii. Nine culture media with different concentration of molasses (M,%) and ammonium sulfate (N,g/l) includings M8N0.5, M8N1.0, M8N1.5, M12N0.5, M12N1.0, M12N1.5, M16N0.5, M16N1.0 and M16N1.5 were randomly assigned in completely randomized design (CRD). Five milliliter of inoculums was transferred in 45 ml of media and incubated at 30oC with shaking at 160 rpm for 6 days. Results illustrated that lipid content was higher when T. asahii was cultured in the medium M12N0.5. This indicated that medium M12N0.5 was suitable for producing single cell oil for use as fat supplement in animal diet |
