SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Effects of in vitro drought stress on growth, proline accumulation and antioxidant defense in sugarcane.

LIMA, J. D.; PEREIRA, M. R.; ROZANE, D. E.; SILVA, S. H. M. G. da; GOMES, E. N.; NOMURA, E. S.; GIACHETTO, P. F..

Banana fruits are susceptible to chilling injury (CI) under field conditions, which significantly impairs fruit quality. Cold tolerance varies among genotypes; however, only a limited number of cultivars have been identified as tolerant and are commercially cultivated. This study aimed to investigate the physiological responses and quality attributes of banana cultivars exposed to natural cold fronts during development, compared with fruits developed under summer conditions. Furthermore, it evaluated whether the B genome confers greater cold tolerance, driven by a more efficient antioxidant mechanism, thereby supporting its recommendation for cultivation in regions prone to low temperatures. Bunches were harvested in winter following five natural cold...

Tipo: Artigo de periódico

Palavras-chave: Estresse por frio; Cultivar de banana; Tolerância ao frio; Estresse oxidativo; Enzimas antioxidantes; Compostos fenólicos; Banana cultivars; Antioxidant enzymes; Banana; Musa sp; Prolina; Cold stress; Cold tolerance; Oxidative stress; Antioxidants; Phenolic compounds; Proline.

Ano: 2026

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1187724

OLIVEIRA, L. A. R. de; CARDOSO, M. N.; OLIVEIRA, A. C. A. de; MACHADO, C. de A.; CARDOSO, B. T.; MUNIZ, A. V. C. da S.; LEDO, A. da S..

Drought is the most limiting environmental factor to crop productivity and presents a great variability in the degree of tolerance among and within species, among varieties. The aim of this study was to characterize sugarcane accessions regarding tolerance to water stress during in vitro cultivation based on changes in biometric, physiological and biochemical characteristics, within species and among species, to support future breeding programs. Adventitious shoots of five sugarcane accessions: Saccharum robustum, Saccharum spontaneum and Saccharum officinarum species, cultivated in Murashige and Skoog medium supplemented with 2% sucrose and 4 g/l Phytagel were used in five water potentials, 0, -0.3, -0.6, -0.9, -1.2 MPa, induced by mannitol. Survival,...

Tipo: Artigo de periódico

Palavras-chave: Cana de Açúcar; Prolina; Absorção de Água; Enzima; Antioxidante; Cane sugar; Drought; Proline; Enzymes; Antioxidants.

Ano: 2018

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1106068

Porta-enxertos promissores, alternativos ao limoeiro 'Cravo', nos Tabuleiros Costeiros de Sergipe.

CARVALHO, L. M. de; CARVALHO, H. W. L. de; SOARES FILHO, W. dos S.; MARTINS, C. R.; PASSOS, O. S..

O objetivo deste trabalho foi identificar porta‑enxertos alternativos ao limoeiro 'Cravo' comum, para produção de laranja 'Pêra', nas condições dos Tabuleiros Costeiros de Sergipe. Foram avaliados 43 porta‑enxertos quanto a altura, copa, taxa fotossintética, teor de prolina, eficiência produtiva, produção e produtividade por planta. O limoeiro 'Cravo' comum foi considerado como padrão. Os porta‑enxertos híbrido TSKC x (LCR x TR) ‑ 059, citrandarim 'Riverside', limoeiro 'Cravo CNPMF ‑ 03' e outros 12 superaram o limoeiro 'Cravo' comum na indução de eficiência produtiva à copa; limoeiro 'Rugoso Vermelho', citrandarim 'Indio' e mais quatro promoveram maior produtividade; TSKC x CTRK ‑ 001 e TSKFL x...

Tipo: Artigo de periódico

Palavras-chave: Deficit hídrico; Limão; Fruta citrica; Híbrido; Prolina; Citrus; Poncirus; Hybrids; Proline.

Ano: 2016

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1056606

Metabolismo do nitrogênio associado à deficiência hídrica e sua recuperação em genótipos de milho.

FERREIRA, V. M.; MAGALHAES, P. C.; DURAES, F. O. M.; OLIVEIRA, L. E. M. de; PURCINO, A. A. C..

O estresse hidrico afeta profundamente o metabolismo celular vegetal. Neste trabalho, objetivou-se quantificar os efeitos da deficiencia hidrica e sua recuperacao sobre a atividade das enzimas do metabolismo do nitrogenio: redutase do nitrato (RN), glutamina sintetase (GS) e glutamato sintase (GOGAT) e sobre o acumulo de prolina em plantas dos genotipos de milho BR 2121 e BR 205. O experimento foi conduzido em casa de vegetacao, sob o delineamento inteiramente casualizado, com quatro repeticoes, utilizando-se vasos que continham 14,3kg de solo. Os tratamentos consistiram da combinacao dos dois genotipos e quatro intervalos entre irrigacoes (1, 3, 5 e 7 dias). No dia da avaliacao (49 dias apos emergencia), os tratamentos com intervalos entre 3 e 7 dias,...

Tipo: Artigo de periódico

Palavras-chave: Estresse hidrico; Redutase de nitrato; Maize; Water deficit; Nitrogênio; Milho; Prolina; Zea Mays; Nitrate reductase; Nitrogen; Proline.

Ano: 2002

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/485227

Biochemical metabolism of young plants of Ucuúba (Virola surinamensis) in the presence of cadmium.

ANDRADE JÚNIOR, W. V.; OLIVEIRA NETO, C. F.; SANTOS FILHO, B. G.; CRUZ, E. D.; AMARANTE, C. B.; VINÍCIUS, S. F.; NOGUEIRA, G. A. S.; NASCIMENTO, V. R.; SOUSA, D. J. P.; TEIXEIRA, J. S. S..

Virola surinamensis is a forest species widely distributed in the estuaries of the Amazon. These ecosystems are susceptible to contamination by Cadmium (Cd), indicating that the plant has strategies for tolerating this metal. The aim of this study was to assess the nitrogen and carbon metabolism of young plants of Ucuúba (Virola surinamensis) in the presence of cadmium with the perspective of the phytoremediation of contaminated environments. The used experimental design was a completely randomized design with five Cd concentrations (0,15, 30, 45, and 60 mg L? 1), for 60 days. In general, Cd did not affect nitrate concentration in the root but had a positive effect on leaves. The reduction of nitrate reductase (NR) in plants exposed to Cd was followed by a...

Tipo: Artigo de periódico

Palavras-chave: Ucuuba; Virola Surinamensis; Nitrate reductase; Proline; Sucrose; Reducing sugars; Cadmium.

Ano: 2021

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131854

Drought tolerance in tough lovegrass (Eragrostis plana): understanding mechanisms and competitive ability.

Drought tolerance and antioxidant enzymatic activity in transgenic 'Swingle' citrumelo plants over-accumulating proline.

BASTIANI, M. O.; GUIDORIZI, K. A.; BENEDETTI, L.; ROCKENBACH, D.; CARATTI, F. C.; ZABALA-PARDO, D. M.; AVILA, L. A.; SOUZA, G. M.; LAMEGO, F. P..

Abstract: Eragrostis plana Nees is a perennial grass weed, native to South Africa and an invasive species of dry rangelands across Asia, Australia, and South America. We hypothesise that E. plana has evolved mechanisms of acclimation to drought conditions to a greater extent than bahiagrass (Paspalum notatum), an important native grass forage species of livestock rangelands of Brazil, leading to a competitive advantage. We measured morphological, physiological, and biochemical response traits in E. plana and P. notatum to water deficit and competition. Levels of drought tolerance in the two species were greatest at the tillering growth stage, driven by low levels of leaf relative water content in E. plana and early stomatal regulation to avoid water loss...

Tipo: Artigo de periódico

Palavras-chave: Regulação estomática; Estresse hídrico; Competitiveness; Stomatal regulation; Eragrostis Plana; Competição de Variedade; Pastagem; Prolina; Recurso Hídrico; Pastures; Proline; Water stress.

Ano: 2024

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1168052

Water relations and organic solutes productions in four umbu tree (Spondias tuberosa) genotypes under intermittent drought.

CAMPOS, M. K. F. de; CARVALHO, K. de; SOUZA, F. S. de; MARUR, C. J.; PEREIRA, L. F. P.; BESPALHOK FILHO, J. C.; VIEIRA, L. G. E..

In this study we investigated the effects of the high endogenous proline level on water relations, gas exchange and antioxidant enzymatic activity in leaves of transgenic 'Swingle' citrumelo rootstocks transformed with the P5CSF129A gene coding for the key-enzyme for proline synthesis, under water deficit. Leaf total water, osmotic and pressure potentials, stomatal conductance, photosynthetic rates and xylem sapflowwere evaluated in non-transformed control and transgenic plants during water deficit treatment. Malondialdehyde (MDA) content, catalase (CAT; EC 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1) and ascorbate peroxidase (APX; EC 1.11.1.11) activities were quantified in leaves collected based on their total water potential, representing the...

Tipo: Artigo de periódico

Palavras-chave: Osmotic adjustment; Citrus rootstock; Drought tolerance; Oxidative stress; Proline.

Ano: 2011

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/902435

The differential expression of the P5CR and αTPS6 genes in cowpea plants increases tolerance to water-deficit and high temperatures.

SILVA, E. C.; NOGUEIRA, R. J. M. C.; VALE, F. H. A.; MELO, N. F. de; ARAUJO, F. P. de.

In order to evaluate changes in leaf water potential (?w) and solute accumulation induced by intermittent drought, a research was performed under greenhouse conditions using four umbu tree genotypes (GBU 44, GBU 48, GBU 50 and GBU 68) and two water treatments (control and stressed by withholding water), with four replicates. The ?w was measured in four-hour intervals during a 24-hour period at the first stomatal closure and at the end of the experimental period. Carbohydrates, amino acids, protein and proline contents were also evaluated in leaves and roots. Significant differences were found in most of the studied parameters. The lower ?w hour was between 800 h and 1200 h. GBU 50 reduced significantly ?w in stressed plants at 800 h, while control lants...

Tipo: Artigo de periódico

Palavras-chave: Bioma Caatinga; Umbuzeiro; Umbu; Genótipo; Spondias Tuberosa; Carboidrato; Potencial Hídrico; Prolina; Water potential; Spondias; Carbohydrates; Proline.

Ano: 2009

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/577914

EFFECT OF FOLIAR APPLICATION OF AMINOACIDS ON PLANT YIELD AND SOME PHYSIOLOGICAL PARAMETERS IN BEAN PLANTS IRRIGATED WITH SEAWATER

BARROS, J. R. A.; GUIMARÃES, M. J. M.; SILVA, R. L. de O.; SILVA, J. B. da; BARROS, A. A. G. de; ANGELOTTI, F.; MELO, N. F. de.

Plants exposed to adverse environmental conditions develop molecular mechanisms of adaptation and/or defense, the osmoprotectors, which function as compatible solutes and contribute to tolerance via prevention systems and protection against cellular damage caused by these abiotic stresses. This study aimed to identify and characterize the osmoprotectors proline and trehalose in cowpea plants cv. Carijó under controlled conditions of water-deficit and heat stress based on the IPCC scenario of 4.8 °C increase in temperature, evaluating their structure and function through computational methods, as well as gene expression by RT-qPCR. The experimental assays were carried out in growth chambers under controlled conditions with different levels of soil water...

Tipo: Artigo de periódico

Palavras-chave: Feijão vigna; Cultura resistente; Estresse abiótico; Osmoprotetores; Agricultura; Vigna Unguiculata; Variedade Resistente; Resistência a Seca; Mudança Climática; Stress; Feijão de Corda; Abiotic stress; Proline; Trehalose; Climate change.

Ano: 2024

URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1164599

Acta biol.Colomb.

SH SADAK,Mervat; ABDELHAMID,Magdi T; SCHMIDHALTER,Urs.

Salinity decreases yield in arid and semi-arid areas. With increasing demand for irrigation water, alternative sources are being sought. Seawater salinity was previously considered unusable for irrigation. However, this water can be used successfully to grow crops under certain conditions. Amino acids is well known biostimulant which has positive effects on plant growth and yield, and significantly mitigates the injuries caused by abiotic stresses. Therefore, in the present study, the effect of exogenously treatment amino acid on faba bean plant growing under seawater salt stress was investigated. Reduction of salinity damage in faba bean by using a mixture of amino acids to improve morphological and biochemical parameters, and thus raising the level of...

URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000100015

Palavras-chave: Free amino acids; Photosynthetic pigments; Proline; Salinity; Vicia faba.

Ano: 2015

Effect of salt stress on physiology and agronomy characterstic of barley cultivars

CIGR Journal

sorkhi, farshad.

In most northwest provinces of Iran such as West Azarbaijan province, soil salinity is a growing problem, particularly in irrigated agricultural areas, and has been found to reduce barley yield, dramatically. To investigate the effect of sodium chloride on two barley (Hordeum vulgare) cultivars, four levels of salinity: 0, 4, 8 and 12 dS/m, were employed as a factorial experiment arranged in a completely randomized design with three replications in a controlled environment of the greenhouse during 2017-2018. The results indicated that increasing salinity from 0 to 12 dS/m, decreased the emergence percentage significantly. Two cultivars of Zarjoo and Valfajr responded differently to salinity, so that Zarjoo showed a significantly higher emergence rate. This...

Effects of substrate water potential in root growth of Agave salmiana Otto ex Salm-Dyck seedlings

Palavras-chave: Emergence; Potassium; Proline; Salinity; Yield components.

Ano: 2020

URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/5855

Biol. Res.

PEÑA-VALDIVIA,CECILIA B; SÁNCHEZ-URDANETA,ADRIANA B.

The objective of this study was to test the hypothesis that root of maguey (Agave salmiana Otto ex Salm-Dyck) seedlings reacts during the first 24 h to low substrate water potential (Yw), by anatomical modifications. Three-4 cm root length seedlings were planted in vermiculite for 24 h at Yw between -0.03 and -2.35 MPa. Root dimensions, proline content and anatomy were evaluated. Substrate ψw between -0.65 and -2.35 MPa did not significantly affect longitudinal root growth. However, proline content significantly increased from 1.6 to 2.1 emoles mg-1. Significant reductions of transverse root area (41 %), thickness of mucilage covering the epidermis (47 %), thickness of epidermis (between 15 and 46 %), area of the parenchyma (between 35 and 41 %)...

Tipo: Journal article

Palavras-chave: Maguey; Proline; Root anatomy; Root growth; Water deficit; Water stress.

Ano: 2009

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200013

Nitric oxide reduces oxidative damage induced by water stress in sunflower plants

Bragantia

Cechin,Inês; Cardoso,Guilherme Sementili; Fumis,Terezinha de Fátima; Corniani,Natália.

Drought is one of the main environmental constraints that can reduce plant yield. Nitric oxide (NO) is a signal molecule involved in plant responses to several environmental stresses. The objective of this study was to investigate the cytoprotective effect of a single foliar application of 0, 1, 10 or 100 µM of the NO donor sodium nitroprusside (SNP) in sunflower plants under water stress. Water stressed plants treated with 1μM SNP showed an increase in the relative water content compared with 0 μM SNP. Drought reduced the shoot dry weight but SNP applications did not result in alleviation of drought effects. Neither drought nor water stress plus SNP applications altered the content of photosynthetic pigments. Stomatal conductance was reduced by drought...

Oxidative Damage and Antioxidant Response in Chenopodium murale L. Exposed to Elevated Levels of Zn

Palavras-chave: Photosynthesis; Reactive oxygen species; Antioxidant enzymes; Proline; Malondialdehyde.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052015000200200

Zoufan,Parzhak; Karimiafshar,Amer; Shokati,Sara; Hassibi,Payman; Rastegarzadeh,Saadat.

ABSTRACT Present study was carried out to investigate the effects of different Zn concentrations on growth and some antioxidant responses in Chenopodium murale L. The seeds were sown on the pots. 45-day plants were transferred to hydroponic solution containing 0.2 (control), 150, 300 and 600 µM Zn. The plants were harvested at 6 days after applying Zn treatments and some parameters were evaluated including plant length, fresh weight, photosynthetic pigments, malondialdehyde (MDA), ascorbate, proline and enzymatic antioxidants such as catalase (CAT), guaiacol peroxidase (GPX) and superoxide dismutase (SOD). Zn concentration showed a considerable increase in the shoot and root as the concentration of Zn increased in the medium, meanwhile the roots were...

An improved method for transformation of lettuce by Agrobacterium tumefaciens with a gene that confers freezing resistance

Palavras-chave: Ascorbate; Enzymatic antioxidant; Lipid peroxidation; Proline; Zinc.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100418

Pileggi,Marcos; Pereira,Albanin Aparecida Mielniczki; Silva,Joandrei dos Santos; Pileggi,Sônia Alvim Veiga; Verma,Desh Pal S..

An efficient method for constructing transgenic lettuce cultivars by Agrobacterium tumefaciens was described by Torres et al., 1993. In the present work, an improvement of the above procedure is described and applied to transform the cultivar Grand Rapids with a mutated P5CS gene. The major modifications were concerned with turning more practical the transformation and regeneration protocols. Also we tried to improve transformation steps by increasing injured area in explants and prolonging co-cultivation with Agrobacteria (in larger concentration). A more significant selective pressure was used against non-transformed plants and bacteria. In these work we were concerned to obtain T1 and T2 seeds. The P5CS gene codes for a delta¹-pyrroline-5-carboxylate...

Proline content and protein patterns in Eucalyptus grandis shoots submitted to high and low temperature shocks

Palavras-chave: Stress resistance; Cold resistance; Transgenic; Lettuce; Proline.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200013

Souza,Gustavo M.; Cardoso,Victor J. M.; Gonçalves,Antonio N..

Proline content and protein patterns changes in response to temperature shocks of both acclimated and non acclimated E. grandis shoots cultivated in vitro were investigated. Analysis of soluble proteins through SDS-PAGE and proline were carried out after 12h at 12ºC (cold acclimation) or 33ºC (heat acclimation), and immediately after temperature shocks at 41ºC and 0ºC. Analyses were also performed 24h after the temperature shocks (recovery period). Temperature treatment at 0ºC did not change soluble protein patterns both in acclimatized and non acclimatized plants, whereas cold temperature induced high proline levels, which kept relatively high after recovery period. Three novel, probably HSPs, proteins (90.5, 75 and 39 kDa) were observed in both...

Study of potential impacts of using sewage sludge in the amendment of desert reclaimed soil on wheat and jews mallow plants

Palavras-chave: Heat shock protein; Proline; Recovery; Stress; Temperature shock.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300004

Mazen,Ahmed; Faheed,Fayza A; Ahmed,Atef F.

This investigation was conducted to study the impacts of using sewage sludge at different concentrations (0, 10, 25, 50, 75%) in amendment of desert reclaimed soil properties and some physiological aspects in wheat and jews mallow plants. Generally adding sewage sludge to desert soil improved the soil texture, raised the organic matter contents, water holding capacity and lowered pH value. The contents of NPK gradually increased as the ratio of sewage sludge increased. The fresh and dry weights and biosyntheses of pigment contents of the variously treated test plants were increased by increasing the sewage sludge levels in the soil. Also, total carbohydrate and protein contents of sewage sludge-treated test plants were positively affected. With respect to...

Isolation of new steroids of Kala Dhaman grass (Cenchrus setigerus) and evaluation of their bioactivity

Palavras-chave: Heavy metals; Protein; Proline; Free amino acids.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400022

Singariya,Premlata; Kumar,Padma; Mourya,Krishan Kumar.

The present study was carried out to determine the possible bioactive components (steroids) of Cenchrus setigerus using GC-MS analysis and in vivo estimation of metabolites (total soluble sugar, soluble protein, proline and total phenolics), photosynthetic pigments (chlorophyll-a, chlorophyll-b and carotenoids) of seedlings and antimicrobial activity of extracts in various polar solvents from the leaves of C. setigerus. Antimicrobial activity was evaluated against three Gram-negative bacteria, including Proteusmirabilis, Klebsiella pneumonia and Agrobacterium tumefaciens andone fungus Aspergillus niger using 'disc diffusion' method, followed by the determination of minimum inhibitory concentrations (MIC) by broth dilution method. Results revealed the...

The effects of salt stress on growth, nitrate reduction and proline and glycinebetaine accumulation in Prosopis alba

Palavras-chave: Cenchrus grass; Photosynthetic pigments; Total soluble sugar; Proline; Antibacterial activity; MIC; MBC/MFC.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100010

Braz. J. Plant Physiol.

Meloni,Diego Ariel; Gulotta,Marta Rosalía; Martínez,Carlos Alberto; Oliva,Marco Antonio.

Prosopis alba (algarrobo) is one of the most important salt-tolerant legumes used in the food and furniture industries. The effects of salinity on some growth and physiological parameters in algarrrobo seedlings were investigated. 17-Day-old seedlings were subjected to three salt treatments by adding NaCl to the growth medium in 50 mmol.L-1 increments every 24 h until the final concentrations of 0, 300 and 600 mmol.L-1 were reached. Only the highest NaCl concentration affected all of the considered parameters. Thus, 600 mmol.L-1 NaCl caused a significant reduction in root and shoot growth, but an increase in the root/shoot ratio. Leaf relative water content, nitrate content and nitrate reductase activity in leaves and roots were also decreased. At 300 and...