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	Provedor de dados BJMBR (2) Ciênc. Tecnol. Aliment. (1) Autor AMARAL,José Eduardo Pedroso Gomes do (1) Canniatti-Brazaca,Solange Guidolin (1) Carneiro,C.R.W. (1) Chemale,G. (1) Farias,S.S. (1) Gimba,E.R.P. (1) Leão,S.C. (1) Matsui,T. (1) TORRES,Larissa Catelli Rocha (1) Zaha,A. (1) Mais... Palavra-chave Promoters (3) Gene expression (2) Actin (1) Caco-2 (1) Cereals (1) Cestode (1) Deletions (1) Echinococcus granulosus (1) Legumes (1) Minerals (1) Mycobacterium tuberculosis (1) Phospholipase C (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2020 (1) 2000 (2) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Cloning and characterization of Echinococcus granulosus (Cestode) EgactI and EgactII actin gene promoters and their functional analysis in the NIH3T3 mouse cell line BJMBR Gimba,E.R.P.; Chemale,G.; Farias,S.S.; Zaha,A.. We report here for the first time the structure and function of a promoter from a cestode. The ability of DNA fragments respectively encompassing the 935-bp and 524-bp regions upstream from the ATG codon from the EgactI and EgactII actin genes of Echinococcus granulosus to promote transcription was studied in the NIH3T3 mouse cell line. The results of transfection assays showed that both regions have strong promoter activity in these cells. The fragments were tested in both orientations and the 524-bp fragment of EgactII presented a bidirectional promoter activity. Deletion analysis of EgactI and EgactII promoters indicated the presence of regulatory regions containing putative silencer elements. These results indicate that both EgactI and EgactII... Tipo: Info:eu-repo/semantics/article Palavras-chave: Echinococcus granulosus; Actin; Promoters; Cestode; Gene expression; Deletions. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100004 Evidence for the expression of native Mycobacterium tuberculosis phospholipase C: recognition by immune sera and detection of promoter activity BJMBR Matsui,T.; Carneiro,C.R.W.; Leão,S.C.. The genome of Mycobacterium tuberculosis H37Rv contains three contiguous genes (plc-a, plc-b and plc-c) which are similar to the Pseudomonas aeruginosa phospholipase C (PLC) genes. Expression of mycobacterial PLC-a and PLC-b in E. coli and M. smegmatis has been reported, whereas expression of the native proteins in M. tuberculosis H37Rv has not been demonstrated. The objective of the present study was to demonstrate that native PLC-a is expressed in M. tuberculosis H37Rv. Sera from mice immunized with recombinant PLC-a expressed in E. coli were used in immunoblots to evaluate PLC-a expression. The immune serum recognized a 49-kDa protein in immunoblots against M. tuberculosis extracts. No bands were visible in M. tuberculosis culture supernatants or... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium tuberculosis; Phospholipase C; Gene expression; Promoters. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100003 Promoters effectiveness in the improvement in iron and zinc absorption from the rice and bean Ciênc. Tecnol. Aliment. TORRES,Larissa Catelli Rocha; AMARAL,José Eduardo Pedroso Gomes do; Canniatti-Brazaca,Solange Guidolin. Abstract Iron and zinc are essential micronutrients for the organism functioning and their lack can result in prejudices to human health. The information about the presence of minerals in the diet are normally referred to its total concentrations, however these values do not reproduce the fraction that might be bioaccessible for the human body. The objective of this study was to evaluate if the promoters increase the iron and zinc bioaccessibility in a rice-and-bean mix. For this, four treatments were elaborated, all containing rice and bean and varied between each other regarding the promoters added to this mix. Ascorbic acid and cysteine were the promoters chosen to evaluate the influence in the iron and zinc absorption. For this purpose, the tomato was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Caco-2; Minerals; Legumes; Cereals; Promoters. Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020005003202 Registros recuperados: 3 Primeira ... 1 ... Última

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