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| Olguín,Hugo C. |
| The transcription factor Pax7 negatively regulates the activity of the muscle regulatory transcription factor MyoD, preventing muscle precursor cells from undergoing terminal differentiation. In this context, the ratio between Pax7 and MyoD protein levels is thought to be critical in allowing myogenesis to proceed or to maintain the undifferentiated muscle precursor state. We have previously shown that Pax7 is subject to rapid down regulation in differentiating myoblasts, via a proteasome-dependent pathway. Here we present evidence indicating that Pax7 is also subject to caspase-3-dependent regulation. Furthermore, simultaneous inhibition of caspase-3 and proteasome activity induced further accumulation of Pax7 protein in differentiating myoblasts. These... |
| Tipo: Journal article |
Palavras-chave: Pax7; Caspase-3; Calpains; Proteasome; Satellite cells; Muscle differentiation. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400002 |
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| Borghetti,Fabian; Noda,Fabio Nakamura; Sá,Cezar Martins de. |
| Freshly harvested sunflower seeds (Helianthus annuus L., Asteraceae) present physiological dormancy localized at the embrionary axis which prevents germination at low temperatures. However, dormant embryos reach about 100 % germination when incubated with the phytohormone ethylene during imbibition. To examine whether proteinase activities are implicated in the breaking of dormancy by ethylene, dormant embryos were treated with the proteinase inhibitors leupeptin, E64 and the proteasome specific inhibitor clasto-lactacystin ß-lactone (lactacystin). A few embryos (< 10 %) germinated at 10 ºC in absence of ethylene but ethylene-treated embryos reached high germinability (~90 %) in the presence of leupeptin, E64, water and DMSO. On the other hand,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Helianthus annuus; Dormancy; Ethylene; Lactacystin; Proteasome. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000200007 |
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