|
|
|
|
|
| Carvalho,Isabela Pena Carvalho de; Detmann,Edenio; Mantovani,Hilário Cuquetto; Paulino,Mário Fonseca; Valadares Filho,Sebastião de Campos; Costa,Viviane Aparecida Carli; Gomes,Daiany Iris. |
| The objective of this study was to evaluate the effects of supplementation with different sources of energy and nitrogenous compounds on the in vitro growth and production of bacteriocin of lactic acid bacteria. Incubations were performed by using ruminal fluid from a rumen-fistulated Holstein-Zebu steer. The animal was kept on a Brachiaria decumbens pasture receiving 200 g/day of supplemental crude protein. Substrates and inoculum were placed in glass flasks considering eight treatments: cellulose, cellulose and casein, cellulose and soy peptone, cellulose and urea, starch, starch and casein, starch and soy peptone and starch and urea. Successive incubations were conducted to select microorganisms according to the energy sources and nitrogenous compounds.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacteriocin; Cellulose; Protein effect; Rumen microbiology. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000600014 |
| |
|
|
| Carvalho,Isabela Pena Carvalho de; Detmann,Edenio; Paulino,Mário Fonseca; Mantovani,Hilário Cuquetto; Valadares Filho,Sebastião de Campos; Gomes,Daiany Iris; Costa,Viviane Aparecida Carli. |
| Two experiments were carried out to evaluate the effect of supplementation with different nitrogenous compounds on the activities of carboxymethil cellulase (CMCase) and glutamate dehydrogenase (GDH). In the first experiment, four treatments were evaluated in vitro: cellulose, cellulose with casein, cellulose with urea, and cellulose with casamino acids. After 6, 12 and 24 hours of incubation, CMCase and GDH activity, pH, and concentrations of ammonia nitrogen (AN) and microbial protein were measured. In the three incubation periods, the concentration of AN was higher when urea was used as a supplemental source of nitrogen. The activity of CMCase was higher with the addition of urea and casamino acids when compared with the control and the casein... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fibrolytic enzymes; Nitrogen assimilation; Protein effect; Rumen microbiology. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000300031 |
| |
|
|
|
