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| Dulermo, Remi; Brunel, Francois; Dulermo, Thierry; Ledesma-amaro, Rodrigo; Vion, Jeremy; Trassaert, Marion; Thomas, Stephane; Nicaud, Jean-marc; Leplat, Christophe. |
| Background The yeast Yarrowia lipolytica is an increasingly common biofactory. To enhance protein expression, several promoters have been developed, including the constitutive TEF promoter, the inducible POX2 promotor, and the hybrid hp4d promoter. Recently, new hp4d-inspired promoters have been created that couple various numbers of UAS1 tandem elements with the minimal LEU2 promoter or the TEF promoter. Three different protein-secretion signaling sequences can be used: preLip2, preXpr2, and preSuc2. Results To our knowledge, our study is the first to use a set of vectors with promoters of variable strength to produce proteins of industrial interest. We used the more conventional TEF and hp4d promoters along with five new hybrid promoters: 2UAS1-pTEF,... |
| Tipo: Text |
Palavras-chave: Yarrowia lipolytica; Protein production; RedStar2; Glucoamylase; Xylanase; Hybrid promoters. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00428/54003/55193.pdf |
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