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Almeida, Manuela. |
Le protozoaire parasite Perkinsus atlanticus (Azevedo, 1989) est une des causes des chutes de production de palourdes Ruditapes decussatus et R. philippinarum cultivées au Portugal, en Espagne et en Italie. Des modèles d'infection de Perkinsus ont été réalisés, par reproduction expérimentale de la maladie, pour étudier le rôle de la densité des palourdes, de la température et de la salinité sur le déroulement de la Perkinsiose. L'objectif de ce travail a été de développer une technique de diagnostic quantitative pour optimiser l'évaluation de l'intensité d'infection et détecter de faibles infections des palourdes par P. atlanticus. Le principe de cette technique consiste à cultiver tous les tissus de la palourde en milieu thioglycolate, suivi d'une lyse à... |
Tipo: Text |
Palavras-chave: Pathologie; Protozoaire; Parasite; Perkinsus atlanticus; Bivalvia; Ruditapes decussatus; Ruditapes philippinarum; Diagnostic. |
Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00033/14416/11710.pdf |
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Mourton, Chantal. |
Oyster culture is, at a world scale, hit by variious epizooties, which are maintly caused by protozoans of the Phylum Ascetospora. Among them, Bonamia ostreae consitutes an appreciated model in order to study, at the cell scale, host-parasite relationships, as well as the immune role of hemocytes. Indeed, this parasite, which reproduces in hemocytes of Ostrea edulis, but cannot develop in Crassostea gigas. This development of an in vitro model for hemocyte-Bonamia relationships is presented. This includes the development and implementation of various techniques : making primocultures, protocol for infection with purified parasites, histological and immunoenzymatic analyses of infection. Such model allowed to study the interaction between host and... |
Tipo: Text |
Palavras-chave: Modèle; Histologie; Méthodologie; Primoculture; Ostrea edulis; Parasite; Hémocytes; Bonamia ostreae; Ascetospora; Protozoaire; Epizootie. |
Ano: 1987 |
URL: http://archimer.ifremer.fr/doc/1987/rapport-3234.pdf |
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Mostajir, B; Bustillos-guzman, J; Claustre, H; Rassoulzadegan, F. |
The egestion of particulate material as well as pigment degradation during microzooplankton grazing on phytoplankton are poorly known processes. In an attempt to evaluate these processes, changes in pigment concentrations within various size fractions were monitored in batch cultures of an assemblage of a pelagic ciliate (Strombidium sulcatum) and a heterotrophic flagellate (Paraphysomonas sp.) feeding on a cyanobacterium (Synechococcus sp.) over a 10-day period. Chlorophyll a, carotenoids and phaeopigments were not found in the 0.1-0.7 mu m fraction while the pigments originally in the 0.7-3.0 mu m fraction (prey) were transferred into the > 3.0 mu m size fraction (predator). During this transfer, the carotenoids (zeaxanthin and B-carotene) were not... |
Tipo: Text |
Palavras-chave: Pigment; Phéopigment; Broutage; Protozoaire; HPLC; Pigment; Phaeopigment; Grazing; Protozoa; HPLC. |
Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00325/43660/43209.pdf |
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