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AVALIAÇÃO DA ATIVIDADE ANTIBACTERIANA E MODULADORA DE AMINOGLICOSÍDEOS DO ÓLEO ESSENCIAL DE Cymbopogon citratus (DC.) Stapf Acta biol.Colomb.
F. LUCENA,Bruno F; TINTINO,Saulo R; FIGUEREDO,Fernando G; DE M. OLIVEIRA,Cícera Datiane; DOS S. AGUIAR,José J; DO N. CARDOSO,Edmilson; A. DE AQUINO,Pedro E; ANDRADE,Jacqueline C; M. COUTINHO,Henrique D; F. MATIAS,Ednardo F.
Vários trabalhos vêm demonstrando a importância do estudo de produtos naturais como fonte alternativa para novos antimicrobianos ou que venham potencializar os já existentes. Neste contexto este trabalho teve como objetivo investigar a atividade antibacteriana e as possíveis interações entre o óleo essencial de Cymbopogon citratus combinados a aminoglicosídeos frente a linhagens padrões e multirresistentes de S. aureus, E. coli e de P. aeruginosa provenientes de isolados clínicos. Um ensaio de microdiluição foi realizado para verificar a atividade antibacteriana e as possíveis interacções entre o produto natural e os antibióticos, utilizando uma concentração sub-inibitória. Através dos resultados foi constatado a interferência sinérgica dos...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cymbopogon citratus; Escherichia coli; Óleo essencial; Pseudomonas aeruginosa; Staphylococcus aureus.
Ano: 2015 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000100005
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Sensibilidad de cepas de Pseudomonas aeruginosa frente a beta-lactámicos ABCL
González Rizo,Aileen; Salazar Rodríguez,Daniel; López Sierra,Odalis; Barrios Martínez,Zoyla; Martínez Izquierdo,Alicia.
Los pacientes portadores de enfermedad fibroquística presentan manifestaciones respiratorias a repetición o crónicas, las cuales se acompañan de infecciones bacterianas. Pseudomonas aeruginosa es la especie bacteriana que se aísla con mayor frecuencia en estos pacientes a partir de muestras de origen respiratorio. Teniendo en cuenta que las infecciones por esta bacteria son difíciles de tratar y que la terapia usualmente empleada es la combinación de un aminoglucósido con un antibiótico beta-lactámico, se analizó el comportamiento por el método de microdilución en caldo de 54 cepas aisladas de pacientes con fibrosis quística frente a cuatro beta-lactámicos (carbenicilina, azlocilina, cefotaxima, ceftazidima), y se observó que el 85% de las cepas fueron...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Fibrosis quística; Beta-lactámicos; Sensibilidad in vitro..
Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572007000100009
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Pseudomonas aeruginosa: un adversario peligroso ABCL
Luján Roca,Daniel Ángel.
Pseudomonas aeruginosa es uno de los patógenos nosocomiales globalmente dominantes; ocasiona una amplia gama de infecciones, algunas tan severas como neumonía o bacteriemia, cuadro que se complica aún más debido a su resistencia intrínseca a diversos antibióticos y a su notable capacidad de adquirir nuevos mecanismos de resistencia, asociándola a elevados índices de mortalidad y convirtiéndola en un serio problema de salud pública.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Resistencia antibiótica; Infecciones.
Ano: 2014 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572014000400009
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Optimización de iones de fierro para la eliminación de piocianina en la reacción de degradación de Tolueno, Benceno y Fenol por Pseudomonas aeruginosa Agrociencia
Rangel-García,María de L.; Rodríguez-Martínez,Jesús; Garza-García,Yolanda; Martínez-Hernández,José L..
Una amplia gama de substancias xenobióticas representan un serio problema como contaminantes ambientales por su toxicidad; entre ellos los compuestos aromáticos pueden ser biodegradados por microorganismos que los usan como fuente de carbono y energía. El género Pseudomonas destaca por sus múltiples aplicaciones biotecnológicas debido a su gran versatilidad metabólica; puede producir metabolitos útiles, transformaciones enzimáticas, biodegradación y biorremediación de suelos, y en aguas contaminadas con petróleo y plaguicidas. En esta investigación se usaron células de P. aeruginosa para degradar tolueno, benceno y fenol. La cepa fue cultivada en medio mineral sólido y se establecieron las concentraciones óptimas para el desarrollo de células viables:...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Degradación; Piocianina; Xenobióticos.
Ano: 2010 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952010000200012
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Tris-EDTA no teste de sensibilidade antimicrobiana in vitro em amostras de Pseudomonas aeruginosa Arq. Bras. Med. Vet. Zootec.
Tanaka,E.M.; Ribeiro,M.G.; Megid,J.; Listoni,F.J.P..
In vitro antimicrobial susceptibility of strains of Pseudomonas aeruginosa by standard diffusion disk test and a modified method, by the addition Tris-EDTA, was evaluated. Increase in sensitivity of agent using modified method was observed mainly in aminoglycosides (amikacin, gentamicin, tobramycin), quinolones (ofloxacin and norfloxacin) and cephalosporins (cefoperazone and ceftazidime) groups. by standard diffusion disk test and a modified method, by the addition Tris-EDTA, was evaluated. Increase in sensitivity of agent using modified method was observed mainly in aminoglycosides (amikacin, gentamicin, tobramycin), quinolones (ofloxacin and norfloxacin) and cephalosporins (cefoperazone and ceftazidime) groups.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Sensibilidade antimicrobiana; Tris-EDTA.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352002000300020
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Modified E-test by the addition of EDTA-Tris and dimethyl sulfoxide on the potentiation of the effects of some antimicrobials in Pseudomonas aeruginosa strains isolated from bovine mastitis Arq. Bras. Med. Vet. Zootec.
Ribeiro,M.G.; Siqueira,A.K.; Langoni,H.; Paes,A.C.; Victória,C.; Silva,A.V. Da; Listoni,F.J.P.; Souza,A.H..
A concentração inibitória mínima-MIC em 30 estirpes de Pseudomonas aeruginosa isoladas de mastite bovina foi avaliada utilizando o E-test padrão e o método modificado, pela adição de Tris-EDTA e DMSO. Os métodos modificados apresentaram redução significativa da MIC das estirpes utilizando a gentamicina, a ciprofloxacina e a norfloxacina.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Mastitis; Pseudomonas aeruginosa; Minimal inhibitory concentration.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000500017
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Evaluation of the heteroantagonism between Enterobacter agglomerans strains isolated from vulture (Coragyps atratus) for production and Pseudomonas aeruginosa as developed Arq. Bras. Med. Vet. Zootec.
Lima,L.A.; Lima,C.D.L.C.; Carvalho,L.R.; Margutti-Pinto,M.E.B..
The heteroantagonism between Enterobacter agglomerans, isolated from the gastrointestinal tract of American vulture Coragyps atratus, and Pseudomonas aeruginosa isolated from a hospital environment was evaluated. The slow (layer) and the wells (direct) techniques were tested, using agar and soy tryptone broth pH 7.3 at 37ºC. Through the slow method from 196 tests, inhibition growth halos, related heteroantagonism phenomenon observed in 118, corresponding to 60.2% positive results. Equivalent positive results were detected using wells (direct) methodology. The seven samples of E. agglomerans tested were capable of revealing heteroantagonism in the experimental conditions; antagonism reveled by the presence of a clear growth inhibition halo. The added 1%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heteroantagonism; Culture; Enterobacter agglomerans; Pseudomonas aeruginosa.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300010
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Synergic interaction between ascorbic acid and antibiotics against Pseudomonas aeruginosa BABT
Cursino,Luciana; Chartone-Souza,Edmar; Nascimento,Andréa Maria Amaral.
Studies were carried out on in vitro combination of ascorbic acid (AA) with six antibiotics against 12 multi-resistant Pseudomonas aeruginosa isolates. Synergic activity was detected with AA chloramphenicol, kanamycin, streptomycin and tetracycline. Indifference was observed to any antibiotics and antagonism only for chloramphenicol. Results indicated that multiresistant P. aeruginosa was affected by combination of AA and antibiotics. Future research on ascorbic acid-antimicrobial interactions may find new methods to control strains of multiresistant P. aeruginosa.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ascorbic acid; Antibiotic; Synergy; Pseudomonas aeruginosa.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300007
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Production of biopolymers by Pseudomonas aeruginosa isolated from marine source BABT
Jamil,Nazia; Ahmed,Nuzhat.
Two bacterial strains, Pseudomonas aeruginosa CMG607w and CMG1421 produce commercially important biopolymers. CMG607w isolated from the sediments of Lyari outfall to Arabian Sea synthesize the mcl-polyhydroxyalkanoates from various carbon sources. The production of PHAs was directly proportional to the incubation periods. Other strain CMG1421, a dry soil isolate, produced high viscous water absorbing extracellular acidic polysaccharide when it was grown aerobically in the minimal medium containing glucose or fructose or sucrose as sole source of carbon. The biopolymer had the ability to absorb water 400 times more than its dry weight. This property was superior to that of currently used non-degradable synthetic water absorbents. It acted as salt filter and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biopolymers; Exopolysaccharides; Hydroabsorbent; Mcl-polyhydroxyalkanoates; Pseudomonas aeruginosa; Sodium gluconate.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300004
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Pseudomonas Aeruginosa: Virulence Factors and Antibiotic Resistance Genes BABT
Rocha,Antônio José; Barsottini,Mario Ramos de Oliveira; Rocha,Renan Rhonalty; Laurindo,Maria Vitória; Moraes,Francisco Leandro Laurindo de; Rocha,Soraya Lília da.
Abstract In this review, we explore some aspects of Pseudomonas aeruginosa virulence factors that are related to disease development in healthy organisms and resistance to antibiotics. This pathogen is one of the most clinically and epidemiologically important bacteria in Brazil, being the major cause of opportunistic infections. Among the virulence factors, biofilm formation acting of manner different in the organism. Furthermore, we review several P. aeruginosa genes that act in antimicrobial resistance, such as β-lactamases against β-lactamers. The resistance to pied-lactamases in P. aeruginosa is associated to resistance to the broad-spectrum cephalosporin. On the other hand, there is a group of synthetic broad-spectrum antibiotics acting on DNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Virulence factors; Antimicrobial activities; Biofilms; Β-lactamase.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100902
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In vitro inhibition of Pseudomonas aeruginosa adhesion by Xylitol BABT
Sousa,Letícia Pinheiro de; Silva,Annelisa Farah da; Calil,Natalia Oliveira; Oliveira,Murilo Gomes; Silva,Silvio Silvério da; Raposo,Nádia Rezende Barbosa.
This study evaluated, in vitro, the antimicrobial activity and the anti-adherent property of xylitol (0.5, 2.5 and 5.0%, w/v) on two Pseudomonas aeruginosa strains (ATCC 9027 and clinical). The assay of antimicrobial activity was performed to determine a minimum inhibitory concentration (MIC) and the adhesion test was performed, by which the parameters regarding, growth in the culture medium, number of colony forming units (CFUs) released and slide evaluation by scanning electron microscopy (SEM) were analyzed. The Statistical Package for the Social Sciences (SPSS) was employed for statistical analysis. Results showed that xylitol had no antimicrobial activity on these strains; however, the inhibition of bacterial adherence was observed in microphotographs...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial adhesion; Pseudomonas aeruginosa; Prevention and control; Xylitol.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500004
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Biofilm production by clinical isolates of Pseudomonas aeruginosa and structural changes in LasR protein of isolates non biofilm-producing BJID
Lima,Jailton Lobo da Costa; Alves,Lilian Rodrigues; Jacomé,Paula Regina Luna de Araújo; Bezerra Neto,João Pacífico; Maciel,Maria Amélia Vieira; Morais,Marcia Maria Camargo de.
ABSTRACT Introduction: Biofilm production is an important mechanism for the survival of Pseudomonas aeruginosa and its relationship with antimicrobial resistance represents a challenge for patient therapeutics. P. aeruginosa is an opportunistic pathogen frequently associated to nosocomial infections, especially in imunocompromised hosts. Objectives: Analyze the phenotypic biofilm production in P. aeruginosa isolates, describe clonal profiles, and analyze quorum sensing (QS) genes and the occurrence of mutations in the LasR protein of non-biofilm producing isolates. Methods: Isolates were tested for biofilm production by measuring cells adherence to the microtiter plates. Clonal profile analysis was carried out through ERIC-PCR, QS genes were by specific...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Biofilm; Multiresistance; Quorum sensing (QS).
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000200129
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Identifying and controlling a multiresistant pseudomonas aeruginosa outbreak in a latin-american cancer centre and its associated risk factors BJID
Cortes,Jorge Alberto; Cuervo,Sonia Isabel; Urdaneta,Ana María; Potdevin,Guillermo; Arroyo,Patricia; Bermúdez,Diana; Correa,Adriana; Villegas,María Virginia.
Pseudomonas aeruginosa is an important and frightening microorganism for patients suffering from cancer. Multiresistant P. aeruginosa (MRPA) may appear as a consequence of exposure to multiple antibiotics or from a breakdown in infection control practices. This article reports an MRPA outbreak in a cancer treatment centre and the consequent case control study. Mechanical ventilation was identified as being the main risk factor for developing MRPA colonisation or infection; molecular analysis confirmed the outbreak. A multifaceted strategy was adopted, involving reinforcing hand-washing practices, contact isolation, antibiotic restriction and suction devices for mechanically-ventilated patients. MRPA was controlled and the outbreak ended. Such strategy may...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Epidemiology; Multiple drug resistance; Bacteria; Risk factor.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000200005
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Factors associated with epidemic multiresistant Pseudomonas aeruginosa infections in a hospital with AIDS-predominant admissions BJID
Gomes,Marisa Zenaide Ribeiro; Oliveira,Raquel Vasconcellos C. de; Machado,Carolina Romero; Conceição,Magda de Souza da; Souza,Cristina Vieira de; Lourenço,Maria Cristina da Silva; Asensi,Marise Dutra.
INTRODUCTION: Infections caused by multiresistant Pseudomonas aeruginosa (MR-PA) have been associated with persistent infections and high mortality in acquired immunodeficiency syndrome (AIDS) patients. Therefore, understanding the predisposing factors for infection/colonization by this agent is critical for controlling outbreaks caused by MR-PA in settings with AIDS patients. OBJECTIVEAND METHODS: To analyze the presence of factors associated with the acquisition of an epidemic MR-PA strain in a hospital with AIDS-predominant admission. A case-control study was carried out in which cases and controls were gathered from a prospective cohort of all hospitalized patients in an infectious disease hospital during a five-year study period. RESULTS: Multivariate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Risk factors; Disease outbreaks; Molecular epidemiology; Acquired immunodeficiency syndrome.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300001
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Pseudomonas aeruginosa infections: factors relating to mortality with emphasis on resistance pattern and antimicrobial treatment BJID
Pinheiro,Millena R.S.; Lacerda,Heloisa R.; Melo,Renata G.L.; Maciel,Mª Amélia.
A retrospective case-control study was conducted to investigate the risk factors for death among intensive care unit patients with Pseudomonas aeruginosa infection. Out of 131 patients investigated, 67 (51.1%) died within 30 days of being diagnosed with this infection. The mean duration of hospital stay before this diagnosis was 28.5 ± 26.5 days. No association was found between bacterial resistance and death in this study (multiresistant p= 0.26; panresistant p= 0.42), but the adequacy of the initial treatment was inversely proportional to the degree of resistance. There was a tendency towards greater mortality among patients who received combination therapy (empirical p= 0.09; definitive p= 0.08), despite the greater frequency of appropriate treatment in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Risk factors; Case-control study; Pseudomonas aeruginosa; Death.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600013
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Antibiotic resistance and molecular typing of Pseudomonas aeruginosa: focus on imipenem BJID
Freitas,Ana Lúcia Peixoto de; Barth,Afonso Luis.
Susceptibility tests by disk diffusion and by E-test and molecular typing by macrorestriction analysis were performed to determine the relatedness of Pseudomonas aeruginosa isolates from three distinct hospitals. The resistance profile of 124 isolates to 8 antimicrobial agents was determined in three different hospitals, in Porto Alegre, Brazil. Frequencies of susceptibility ranged from 43.9% for carbenicillin to 87.7% for ceftazidime. Cross-resistance data of imipenem-resistant isolates indicated that most (70%) were also resistant to carbenicillin, although 30% remained susceptible to ceftazidime and cefepime. In general, susceptibility profiles were not able to determine relatedness among isolates of P. aeruginosa. On the other hand, molecular typing by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Antibiotic resistance; Imipenem.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000100001
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An outbreak of conjunctivitis caused by multiresistant Pseudomonas aeruginosa in a Brazilian Newborn Intensive Care Unit BJID
Brito,D. V. D.; Oliveira,E. J.; Matos,C.; Abdallah,V. O.S.; Gontijo Filho,P.P..
We report an outbreak of conjunctivitis due to Pseudomonas aeruginosa involving seven infants admitted in the Neonatal Intensive Care Unit (NICU) of the Uberlândial Federal University Hospital between March and September 2001. Three infants developed systemic complications (01 sepsis and 02 pneumonias). Ten isolates were obtained from conjunctival cultures and all were resistant to ceftazidime and aminoglycosides. Fast identification of the organism and treatment with imipenem were important in containing the outbreak of P. aeruginosa.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Pseudomonas aeruginosa; Nosocomial infection; Neonatal unit.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000400001
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Molecular typing and biological characteristics of Pseudomonas aeruginosa isolated from cystic fibrosis patients in Brazil BJID
Stehling,Eliana Guedes; Leite,Domingos S; Silveira,Wanderley D.
The present study had as objective to evaluate the genotypic diversity and biological characteristics, such as hemolysin, protease, elastase of 56 clinical strains of Pseudomonas aeruginosa isolated from 13 cystic fibrosis (CF) patients attending at the School Hospital of Campinas State University (UNICAMP), Brazil. Genotypic diversity has been determined by Ribotyping (RT) and the pattern of the enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) of each strain. The production of elastase was significantly different only among mucoid and nonmucoid isolates. Joint results obtained by (RT) and ERIC-PCR methods were able to discriminate all strains isolated from both the same and different patients. Additionally, we observed four strain clusters...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cystic fibrosis; Pseudomonas aeruginosa; Clonal analysis; Biological characteristics.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000500007
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Prevalence of carbapenem resistant Pseudomonas aeruginosa and Acinetobacter baumannii in high complexity hospital BJID
Baumgart,Ana Milda Karsten; Molinari,Marcelo André; Silveira,Alessandro Conrado de Oliveira.
Pseudomonas aeruginosa and Acinetobacter baumannii are Gram-negative bacilli that in the last decades have become prevalent agents of hospital infection due to high antimicrobial resistance developed by these microorganisms. The present study is a retrospective analysis of all positive cultures for these microorganisms in the period of January 2004 to December 2008. Resistance levels of A. baumannii and P. aeruginosa to carbapenems was high and showed a trend to increase during the period of study. In recent years the increasing incidence and resistance levels of A. baumannii and P. aeruginosa to the antimicrobials used for their treatment in the hospital setting underscores the relevance of infections caused by these bacteria. The selective pressure...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acinetobacter baumannii; Pseudomonas aeruginosa; Drug resistance; Carbapenems; Hospital infection control program.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000500002
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Infections after shoulder arthroplasty are correlated with higher anesthetic risk score: a case-control study in Brazil BJID
Nagaya,Leonardo Hideto; Salles,Mauro José Costa; Takikawa,Lucas Sadawo Chagas; Fregoneze,Marcelo; Doneux,Pedro; Silva,Luciana Andrade da; Sella,Guilherme do Val; Miyazaki,Alberto Naoki; Checchia,Sergio Luiz.
ABSTRACT Purposes: Shoulder arthroplasty (SA) has been performed by many years for the treatment of several conditions, including osteoarthritis and proximal humeral fractures following trauma. Surgical site infection (SSI) following Shoulder arthroplasty remains a challenge, contributing to increased morbidity and costs. Identification of risk factors may help implementing adequate strategies to prevent infection. We aimed to identify pre- and intra-operative risk factors associated with deep infections after Shoulder arthroplasty. Methods: An unmatched case-control study was conducted to describe the prevalence, clinical and microbiological findings, and to evaluate patient and surgical risk factors for prosthetic shoulder infection (PSI), among 158...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Orthopedic infection; Hemiarthroplasty; Total shoulder arthroplasty; American Society of Anesthesiologists score; Risk factor; Pseudomonas aeruginosa.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600613
Registros recuperados: 69
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