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Efficient production of pullulan by Aureobasidium pullulans grown on mixtures of potato starch hydrolysate and sucrose BJM
An,Chao; Ma,Sai-jian; Chang,Fan; Xue,Wen-jiao.
Abstract Pullulan is a natural exopolysaccharide with many useful characteristics. However, pullulan is more costly than other exopolysaccharides, which limits its effective application. The purpose of this study was to adopt a novel mixed-sugar strategy for maximizing pullulan production, mainly using potato starch hydrolysate as a low-cost substrate for liquid-state fermentation by Aureobasidium pullulans. Based on fermentation kinetics evaluation of pullulan production by A. pullulans 201253, the pullulan production rate of A. pullulans with mixtures of potato starch hydrolysate and sucrose (potato starch hydrolysate:sucrose = 80:20) was 0.212 h−1, which was significantly higher than those of potato starch hydrolysate alone (0.146 h−1) and mixtures of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mixed sugar; Aureobasidium pullulans; Pullulan; Potato starch hydrolysate; Batch kinetics.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100180
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Production of pullulan by a thermotolerant Aureobasidium pullulans strain in non-stirred fed batch fermentation process BJM
Singh,Ranjan; Gaur,Rajeeva; Tiwari,Soni; Gaur,Manogya Kumar.
Total 95 isolates of Aureobasidium pullulans were isolated from different flowers and leaves samples, out of which 11 thermotolerant strains produced pullulan. One thermotolerant non-melanin pullulan producing strain, designated as RG-5, produced highest pullulan (37.1±1.0 g/l) at 42ºC, pH 5.5 in 48h of incubation with 3% sucrose and 0.5% ammonium sulphate in a non-stirred fed batch fermentor of 6 liters capacity. The two liters of initial volume of fermentation medium was further fed with the 2 liters in two successive batches at 5 h interval into the fermentor. The sterile air was supplied only for 10h at the rate of 0.5 vvm.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aureobasidium pullulans; Pullulan; Non-stirred fed-batch fermentation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300030
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Pullulan as a stabilizer agent of polymeric nanocapsules for drug delivery BJPS
Chassot,Janaíne Micheli; Ferreira,Luana Mota; Gomes,Felipe Pereira; Silva,Cristiane de Bona da; Tasso,Leandro; Cruz,Letícia.
ABSTRACT Polymeric stabilizers have received attention in the preparation of nanostructured systems due to their ability to enhance formulation stability. Considering this, the objective of this work was to prepare poly(ε-caprolactone) nanocapsules using the pullulan as a polymeric stabilizer. The nanocapsules were prepared using the interfacial deposition method of preformed polymers and they were characterized in terms of pH, average diameter, polydispersity index, zeta potential, beclomethasone dipropionate content, encapsulation efficiency, photostability and drug release profiles. The formulations showed physicochemical characteristics consistent with nanocarriers for drug delivery such as: average diameter lower than 270 nm, polydispersity indexes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pullulan; Nanocapsules/diffusion technique; Pullulan/polymeric stabilizer; Biocompatible polymers; Beclomethasone dipropionate/nanocapsules..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400735
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Pullulan: an advantageous natural polysaccharide excipient to formulate tablets of alendronate-loaded microparticles BJPS
Ferreira,Luana Mota; Velasquez,Aline de Arce; Schaffazick,Scheila Rezende; Cruz,Letícia.
This work reports the preparation of tablets by direct compression of sodium alendronate-loaded microparticles, using pullulan as filler. The tableting properties of pullulan were compared with those of microcrystalline cellulose and lactose. Pullulan tablets showed low variations in average weight, thickness and drug content. Moreover, these tablets exhibited a higher hardness compared to the other excipients. In vitro release studies showed that only pullulan was capable to maintain gastroresistance and release properties of microparticles, due to its ability to protect particles against damage caused by compression force. Thus, pullulan was considered an advantageous excipient to prepare tableted microparticles.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pullulan; Excipients; Microparticulate tablets; Sodium alendronate.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100027
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