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Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm PAB
Carneiro,Andréa Almeida; Gomes,Eliane Aparecida; Guimarães,Claudia Teixeira; Fernandes,Fernando Tavares; Carneiro,Newton Portilho; Cruz,Ivan.
The objective of this work was to evaluate the pathogenicity of 24 Beauveria isolates to Spodoptera frugiperda larvae, and characterize them molecularly through rDNA-ITS sequencing and RAPD markers. Sequencing of rDNA-ITS fragments of 570 bp allowed the identification of isolates as B. bassiana or B. brongniarti by sequence comparison to GenBank. Sixty seven polymorphic RAPD fragments were capable to differentiate 20 among 24 Beauveria isolates, grouping them according to the derived host insect and to pathogenicity against maize fall armyworm larvae. Three RAPD markers were highly associated to the pathogenicity against S. frugiperda, explaining up to 67% of the phenotypic variation. Besides identification and molecular characterization of Beauveria...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spodoptera frugiperda; Entomopathogenic fungi; RAPD; RDNA-ITS.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000400010
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