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| Yu,Ying; Zhou,Xiaoyu; Wu,Sheng; Wei,Tiantian; Yu,Long. |
| Background Lysozyme plays a crucial role in innate immunity with its well-recognized bacteriolytic activity. In this study, the influence of expression parameters (inoculation volume, culture volume, growth time, induction temperature and time, initial pH and methanol concentration) on human lysozyme (HLZ) production in recombinant P. pastoris SMD1168 was investigated through Plackett-Burman (PB) design and response surface methodology (RSM). Results It was revealed that induction temperature, induction time and culture volume had significant influence (P < 0.01) on HLZ expression level, which were elected for further optimization with three-dimensional response surface designs for enhanced HLZ production. The highest lysozyme activity reached 3301 U/mL... |
| Tipo: Journal article |
Palavras-chave: Expression optimization; LYZ; Plackett-Burman design; RSM. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600009 |
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| Khayer, Shadad Md; Patel, Thaneswer; Dewangan, Krishna Narayan. |
| Paddy is the main crop in India grown in about 44 million ha area. Threshing of paddy is one of the most widely used practice in India. Traditional methods of threshing are still prevalent in most of the parts of North-Eastern region. The farming community of the North-Eastern region faces some difficulties in adoption due to low productivity, inadequate design, and injuries during threshing.This study made an attempt to optimize the most significant design parameter of pedal operated paddy thresher (POPT) such as loop spacing, tip height, drum peripheral speed and number of the strip in the periphery of drum to achieve the optimum threshing capacity using Response surface methodology (RSM) in Design Expert v7 tool. From the analysis of variance (ANOVA),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paddy Threshing; POPT; Machine parameters; RSM; Optimize. |
| Ano: 2019 |
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/4713 |
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| Lalitendu Das; North Carolina State University Biological and Agricultural Engineering; Praveen Kolar; North Carolina State University; Jason Osborne; North Carolina State University Department of Statistics; John Classen; North Carolina State University Biological and Agricultural Engineering. |
| Swine farming emit several odorous volatile organic compounds, one of which is p-cresol. Considering the layout of a swine barn, adsorption is one of the most suitable technologies for mitigating organic pollutants. In this study, commercial granular activated carbon (GAC) was tested as an adsorbent for removing p-cresol from aqueous solution. The objectives were to: 1) determine the combined effect of temperature, pH, and adsorbent dose on adsorption, (2) investigate the effect of volatile fatty acids and aldehydes on adsorption of p-cresol, (3) determine adsorption kinetics and isotherms, (4) study the effect of solvent on adsorption, (5) propose a possible mechanism of adsorption, and (6) discuss practical implications and design calculations for... |
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Palavras-chave: Agricultural Engineering Adsorption; P-cresol; Activated carbon; Kinetics; Isotherms; RSM. |
| Ano: 2012 |
URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/2171 |
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| BANERJEE,GOUTAM; GORTHI,SRIKANTH; CHATTOPADHYAY,PRITAM. |
| ABSTRACT Disease in agricultural field is a big problem that causes a massive loss in production. In this present investigation, we have reported a soil-borne bacterium Bacillus cereus IB311 which is antagonistic to plant pathogens (Pseudomonas syringae and Agrobacterium tumefaciens), and could make a substantial contribution to the prevention of plant diseases. To prove the practical application, the strain was directly applied in agricultural field. The results demonstrated that B. cereus IB311 has increased the production (20% and 26% in term of average pod number per plant, average seed number per pod, and seed yield per experimental plot) in ground nut (Arachis hypogaea var. Koushal, G201) and sesame (Sesamum indicum var. Kanak), respectively. To... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus cereus; Bio-control; Phytopathogens; RSM; Field application; Crop production. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502149 |
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| Stergiou,Panagiota-Yiolanda; Foukis,Athanasios; Theodorou,Leonidas; Papagianni,Maria; Papamichael,Emmanuel. |
| The aim of this work was to study the production of extracellular α-amylase by Kluyveromyces marxianus IF0 0288 using optimized nutritional and cultural conditions in a complex yeast medium under aerobic batch fermentation. By applying the conventional "one-variable-at-a-time" approach and the response surface methodology, the effect of four fermentation parameters (type of carbon source, initial culture pH, temperature, and incubation time) on the growth and α-amylase production was evaluated. The production of α-amylase during 60 h of fermentation increased 13-fold under optimized conditions (1% starch, pH 6.0, 30ºC) in comparison to the conventional optimization method. The initial pH value of 6.13 and temperature of 30.3ºC were optimal conditions by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Extracellular enzymes; Α-amylase; Kluyveromyces marxianus; Fermentation; RSM. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300016 |
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