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| CAVALCANTI,MARIANA B.; FERNANDES,THIAGO S.; SILVA,EDVANE B.; AMARAL,ADEMIR. |
| The aim of this research was to evaluate the relationship between p53 protein levels and absorbed doses from in vitro irradiated human lymphocytes. For this, samples of blood from 23 donors were irradiated with 0.5; 1; 2; and 4 Gy from a Cobalt-60 source, and the percentages of lymphocytes expressing p53 were scored using Flow Cytometry. The subjects were divided into 3 groups, in accordance with the p53 levels expressed per radiation dose: low (Group I), high (Group II), and excessive levels (Group III). For all groups, the analyses showed that the p53 expression levels increase with the absorbed dose. Particularly for groups I and II, the correlation between this protein expression and the dose follows the linear-quadratic model, such as for radioinduced... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological Dosimetry; Cellular radiobiology; Flow Cytometry; P53 protein; Radiosensitivity. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401783 |
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| LOPES,AMANDA M.; BOBROWSKI,VERA L.; SILVA,SERGIO D. DOS ANJOS E; DEUNER,SIDNEI. |
| ABSTRACT This study aimed to evaluate the radiosensitivity of castor bean seeds after applications of different doses of Cobalt60 gamma radiation. Seeds were pre-soaked for 24 hours in distilled water and then irradiated with 50, 100, 150, and 200 Gy, except the control. Sowing was performed in trays, which contained soil as substrate and were maintained in a greenhouse. The electrical conductivity, emergence, emergence speed index, growth parameters and activities of antioxidant enzymes (superoxide dismutase, ascorbate peroxidase, and catalase) were evaluated in the leaves and roots of castor bean seedlings. Gamma radiation did not affect the electrical conductivity of the seeds; however, at a dose of 200 Gy, the emergence and emergence speed index of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Castor bean; Radiosensitivity; Germination; Growth; Antioxidant enzymes. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000401925 |
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| Zhang,Xia; Li,Yuehua; Wang,Dan; Wei,Xiaoer. |
| Abstract Background miR-22 has been shown to be frequently downregulated and act as a tumor suppressor in multiple cancers including breast cancers. However, the role of miR-22 in regulating the radioresistance of breast cancer cells, as well as its underlying mechanism is still not well understood. Methods The expressions of miR-22 and sirt1 at mRNA and protein levels were examined by qRT-PCR and Western Blot. The effects of miR-22 overexpression and sirt1 knockdown on cell viability, apoptosis, radiosensitivity, γ-H2AX foci formation were evaluated by CCK-8 assay, flow cytometry, colony formation assay, and γ-H2AX foci formation assay, respectively. Luciferase reporter assay and qRT-PCR analysis were performed to confirm the... |
| Tipo: Journal article |
Palavras-chave: MiR-22; Tumorigenesis; Radiosensitivity; Breast cancer; Sirt1. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100220 |
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| Joksic,G.; Petrovic,S.; Ilic,Z.. |
| The aim of the present study was to establish the extent of in vitro radioresponse of lymphocytes among 62 healthy adults of both genders and to estimate the distribution of baseline micronuclei and radiosensitivity among individuals of the study population using the cytochalasin block micronucleus test. A younger study group consisted of 10 males (mean age, 22.4 years; range, 21-27) and 12 females (mean age, 24.8 years; range, 20-29), whereas an older study group consisted of 18 males (mean age, 35.1 years; range, 30-44) and 22 females (mean age, 38.5 years; range, 30-48). For evaluation of radiosensitivity blood samples were irradiated in vitro using 60Co g-ray source. The radiation dose employed was 2 Gy, the dose rate 0.45 Gy/min. The study revealed a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human lymphocytes; Baseline micronuclei; Radiosensitivity; Ageing. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000800002 |
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