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	Provedor de dados BJMBR (2) Electron. J. Biotechnol. (2) BJM (1) Genet. Mol. Biol. (1) Autor Fu,Junjiang (2) Khan,Asaduzzaman (2) Araújo-Júnior,João P. (1) Ashayeri-Panah,Mitra (1) Carvalho,V.M. (1) Castro,A.F. Pestana de (1) Cheng,Jingliang (1) Damasco,P.V. (1) Eftekhar,Fereshteh (1) Feizabadi,Mohammad Mehdi (1) Filardy,A.A. (1) Fu,Shelly (1) Ghamsari,Maryam Mobarak (1) Gomes,D.L.R. (1) Hirata Jr.,R. (1) Irino,K. (1) Long,Yan (1) Luo,Peiyi (1) Mattos-Guaraldi,A.L. (1) Mei,Zhiqiang (1) Mais... Palavra-chave Random amplified polymorphic DNA (6) Genetic authentication (2) Aedes aegypti (1) Aggregative adherence (1) Angelica sinensis (1) Attaching and effacing (1) Attaching and effacing Escherichia coli (1) Corynebacterium diphtheriae (1) Endocarditis (1) Enteropathogenic Escherichia coli (1) Escherichia coli (1) Genetic variability (1) Genotyping (1) HEp-2 cells (1) Inter-simple sequence repeat (1) Klebsiella pneumoniae (1) Molecular cloning (1) Monkey (1) Population genetics (1) Pulsed field gel electrophoresis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Journal article (2) Info:eu-repo/semantics/other (1) Ano 2015 (2) 2013 (1) 2008 (1) 2007 (1) 2006 (1) País Brazil (4) Chile (2) Idioma Inglês (6)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 6 Primeira ... 1 ... Última Efficiency of improved RAPD and ISSR markers in assessing genetic diversity and relationships in Angelica sinensis (Oliv.) Diels varieties of China Electron. J. Biotechnol. Mei,Zhiqiang; Zhang,Chun; Khan,Asaduzzaman; Zhu,Ye; Tania,Mousumi; Luo,Peiyi; Fu,Junjiang. Background Angelica sinensis is a well-known traditional Chinese medicinal plant. We aimed to assess the genetic diversity and relationships in A. sinensis cultivars collected from different locations of China and also some other Angelica species. Results We employed an improved random amplified polymorphic DNA (RAPD) technique for the amplification of DNA materials from ten Angelica cultivars, and the results were verified by inter-simple sequence repeat (ISSR) analysis. Twenty six RAPD primers were used for RAPD, and the amplified bands were found highly polymorphic (96%). Each primer amplified 8-14 bands with an average of 10.25. The cluster dendrogram showed that the similarity coefficients ranged from 0.41 to 0.92. The similarity coefficients were... Tipo: Journal article Palavras-chave: Angelica sinensis; Genetic authentication; Inter-simple sequence repeat; Random amplified polymorphic DNA; Substitutes. Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200006 Development and significance of RAPD-SCAR markers for the identification of Litchi chinensis Sonn: by improved RAPD amplification and molecular cloning Electron. J. Biotechnol. Cheng,Jingliang; Long,Yan; Khan,Asaduzzaman; Wei,Chunli; Fu,Shelly; Fu,Junjiang. Background Analysis of genetic diversity is important for the authentication of a species. Litchi (Litchi chinensis Sonn.) is a subtropical evergreen tree. Recently, L. chinensis has been characterized by an improved random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analysis. The goal of this study was to develop sequence-characterized amplified region (SCAR) markers from the improved RAPD fragments for the genetic analysis of L. chinensis. Results The improved RAPD fragments from L. chinensis were cloned, sequenced and converted into stable SCAR markers. Sequencing of three cloned RAPD fragments revealed that the clone L7-16 consisted of 222 nucleotides (GenBank accession number KM235222), clone L9-6 consisted of 648... Tipo: Journal article Palavras-chave: Genetic authentication; Molecular cloning; Random amplified polymorphic DNA; Sequence-characterized amplified region marker. Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100007 Genetic variability in geographical populations of Aedes aegypti (Diptera, Culicidae) in Brazil elucidated by molecular markers Genet. Mol. Biol. Paduan,Karina dos Santos; Araújo-Júnior,João P.; Ribolla,Paulo E.M.. The tropical mosquito, Aedes aegypti is the most important domestic vector of urban yellow fever and dengue. Genetic population studies on this vector are important because they may lead to new tools for surveillance. An analysis of genetic structure was conducted among populations of A. aegypti from 11 localities in four demographic regions within six Brazilian federal states. Markers included 21 random amplified polymorphic DNA (RAPD) loci. RAPD markers were detected among populations and cluster analysis revealed two main groups. We found high genetic polymorphism (H S = 0.224) and high levels of genetic differentiation between populations from different states (G ST = 0.430), as well as in populations from cities in the same state (G ST = 0.410). These... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aedes aegypti; Population genetics; Random amplified polymorphic DNA; Genetic variability. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200030 Random amplification of polymorphic DNA reveals clonal relationships among enteropathogenic Escherichia coli isolated from non-human primates and humans BJMBR Carvalho,V.M.; Irino,K.; Onuma,D.; Castro,A.F. Pestana de. Enteropathogenic Escherichia coli (EPEC) strains are important agents of infantile diarrhea all over the world, gaining even greater importance in developing countries. EPEC have also been isolated from various animal species, but most isolates belong to serotypes that differ from those recovered from humans. However, it has been demonstrated that several isolates from non-human primates belong to the serogroups and/or serotypes related to those implicated in human disease. The objective of this study was to evaluate the genetic differences between thirteen strains isolated from non-human primates and the same number of strains isolated from human infections. Human isolates belonged to the same serogroup/serotype as the monkey strains and the evaluation... Tipo: Info:eu-repo/semantics/other Palavras-chave: Escherichia coli; Enteropathogenic Escherichia coli; Attaching and effacing Escherichia coli; Attaching and effacing; Monkey; Random amplified polymorphic DNA. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200010 Potential pathogenic role of aggregative- adhering Corynebacterium diphtheriae of different clonal groups in endocarditis BJMBR Hirata Jr.,R.; Pereira,G.A.; Filardy,A.A.; Gomes,D.L.R.; Damasco,P.V.; Rosa,A.C.P.; Nagao,P.E.; Pimenta,F.P.; Mattos-Guaraldi,A.L.. Invasive diseases caused by Corynebacterium diphtheriae have been described increasingly. Several reports indicate the destructive feature of endocarditis attributable to nontoxigenic strains. However, few reports have dealt with the pathogenicity of invasive strains. The present investigation demonstrates a phenotypic trait that may be used to identify potentially invasive strains. The study also draws attention to clinical and microbiological aspects observed in 5 cases of endocarditis due to C. diphtheriae that occurred outside Europe. Four cases occurred in female school-age children (7-14 years) treated at different hospitals in Rio de Janeiro, Brazil. All patients developed other complications including septicemia, renal failure and/or arthritis.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aggregative adherence; Corynebacterium diphtheriae; Endocarditis; HEp-2 cells; Random amplified polymorphic DNA. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100007 Genetic profiling of Klebsiella pneumoniae: comparison of pulsed field gel electrophoresis and random amplified polymorphic DNA BJM Ashayeri-Panah,Mitra; Eftekhar,Fereshteh; Ghamsari,Maryam Mobarak; Parvin,Mahmood; Feizabadi,Mohammad Mehdi. In this study, the discriminatory power of pulsed field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) methods for subtyping of 54 clinical isolates of Klebsiella pneumoniae were compared. All isolates were typeable by RAPD, while 3.6% of them were not typeable by PFGE. The repeatability of both typing methods were 100% with satisfying reproducibility (≥ 95%). Although the discriminatory power of PFGE was greater than RAPD, both methods showed sufficient discriminatory power (DI > 0.95) which reflects the heterogeneity among the K. pneumoniae isolates. An optimized RAPD protocol is less technically demanding and time consuming that makes it a reliable typing method and competitive with PFGE. Tipo: Info:eu-repo/semantics/article Palavras-chave: Klebsiella pneumoniae; Pulsed field gel electrophoresis; Random amplified polymorphic DNA; Genotyping. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300024 Registros recuperados: 6 Primeira ... 1 ... Última

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