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	Provedor de dados BJM (2) Autor Achí,Rosario (1) Dodd,Christine E.R. (1) Jiménez,Kenia Barrantes (1) McCoy,Clyde B. (1) Rees,Catherine E.D. (1) Siqueira,Regina Silva de (1) Mais... Palavra-chave Rapid method (2) Bacteriophage (1) Food (1) Lettuce (1) P22 (1) PCR (1) Salmonella (1) Shigella (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2010 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Phage amplification assay as rapid method for Salmonella detection BJM Siqueira,Regina Silva de; Dodd,Christine E.R.; Rees,Catherine E.D.. The application of rapid methods is crucial for the HACCP program implantation in food industry. In this context, Phage Amplification Assay is a good candidate because is based on the interactions of phage and their host bacteria. This method using phage P22 was applied with to detect Salmonella cells in chicken breast. Samples of 25 g of chicken breast were diluted and the appropriate dilutions were used in phage amplification assay for Salmonella detection. After 3-4 h of incubation, it was observed a phage titre of approximately 10(4) pfu mL-1, indicating that there were Salmonella cells which were naturally present in the meat. The presence of Salmonella cells were verified by using direct plating on XLD agar and by conventional enrichment procedure.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Rapid method; Bacteriophage; P22. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500040 Detection of shigella in lettuce by the use of a rapid molecular assay with increased sensitivity BJM Jiménez,Kenia Barrantes; McCoy,Clyde B.; Achí,Rosario. A Multiplex Polymerase Chain Reaction (PCR) assay to be used as an alternative to the conventional culture method in detecting Shigella and enteroinvasive Escherichia coli (EIEC) virulence genes ipaH and ial in lettuce was developed. Efficacy and rapidity of the molecular method were determined as compared to the conventional culture. Lettuce samples were inoculated with different Shigella flexneri concentrations (from 10 CFU/ml to 10(7) CFU/ml). DNA was extracted directly from lettuce after inoculation (direct-PCR) and after an enrichment step (enrichment PCR). Multiplex PCR detection limit was 10(4) CFU/ml, diagnostic sensitivity and specificity were 100% accurate. An internal amplification control (IAC) of 100 bp was used in order to avoid false... Tipo: Info:eu-repo/semantics/article Palavras-chave: PCR; Shigella; Rapid method; Lettuce; Food. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400018 Registros recuperados: 2 Primeira ... 1 ... Última

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