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	Provedor de dados BJM (4) Autor Aleixo,José Antonio Guimarães (1) Barros,Márcia de Aguiar Ferreira (1) Beloti,Vanerli (1) Carvalhal,José Beiro (1) Conceição,Rita de Cássia dos Santos da (1) Destro,Maria Teresa (1) Ferrati,Analí Ramazotti (1) Franco,Bernadette Dora Gombossy de Melo (1) Goularte,Fabiana Lemos (1) Gusmão,Viviane Vieira (1) Landgraf,Mariza (1) Li,Fan (1) Moraes,Luciane Bilia de (1) Moreira,Ângela Nunes (1) Nero,Luís Augusto (1) Pereira,Mykel Steffani (1) Ramos,Roberta Juliano (1) Santana,Elsa Helena Walter de (1) Tavolaro,Paula (1) Wang,Haihe (1) Mais... Palavra-chave Rapid methods (4) Detection (1) Diagnosis (1) Goat milk (1) Identification (1) Immunomagnetic separation (1) Milk (1) Mycobacterium tuberculosis complex (MTBC) (1) PetrifilmTM AC (1) Ready-to-use systems (1) Salmonella (1) SimPlate (1) SimplateTM TPC (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Ano 2011 (1) 2008 (1) 2005 (1) 2002 (1) País Brazil (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Rapid identification of Mycobacterium tuberculosis complex by a novel hybridization signal amplification method based on self-assembly of DNA-streptavidin nanoparticles BJM Wang,Haihe; Zhao,Chunyan; Li,Fan. Rapid detection of Mycobacterium tuberculosis complex (MTBC) is a critical step in controlling tuberculosis (TB). In this study, we used IS6110 as the specific identification target to develop a novel hybridization signal amplification method (HSAM) for the rapid and direct detection of MTBC from clinical sputum specimens. This system consists of magnetic bead-linked capture probes for target isolation, dextranbased nanoparticles for amplifying the reporter molecule (biotinylated-FITC), and detection probes (2B-DNA) for binding the nanoparticles. Both the capture and detection probes were specific to the IS6110 target sequence. Our results determined that as few as 10 copies of the IS6110 sequence or 10 M. tuberculosis bacteria could be detected,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium tuberculosis complex (MTBC); Detection; Diagnosis; Rapid methods; Identification. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300016 Assessment of the efficiency of SimPlate™ total plate count color indicator (TPC CI) to quantify mesophilic aerobic microorganisms in pasteurized milk BJM Nero,Luís Augusto; Beloti,Vanerli; Barros,Márcia de Aguiar Ferreira; Santana,Elsa Helena Walter de; Pereira,Mykel Steffani; Gusmão,Viviane Vieira; Moraes,Luciane Bilia de. The SimPlate™ TPC CI system is a rapid method to count mesophilic aerobic microorganisms (MAM) in foods, based on the use of resazurine to indicate bacterial growth. Its efficiency in pasteurized milk was evaluated using 142 pasteurized milk samples (38 type A, 43 type B and 61 type C) collected in Londrina, PR. The standard plating method, using Plate Count Agar (PCA) was used for comparison. The plates of both systems were incubated at 35ºC and read after 24h and 48h. The occurrence of false-positive and false-negative wells and the predominant microorganisms in them were also evaluated. The results were compared by simple correlation and mean variance analyses. The correlation (r) and mean variance values were 0.6811 and 0.7583 for the results obtained... Tipo: Info:eu-repo/semantics/article Palavras-chave: Rapid methods; Milk; SimPlate. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100009 Detection of salmonella sp in chicken cuts using immunomagnetic separation BJM Conceição,Rita de Cássia dos Santos da; Moreira,Ângela Nunes; Ramos,Roberta Juliano; Goularte,Fabiana Lemos; Carvalhal,José Beiro; Aleixo,José Antonio Guimarães. The immunomagnetic separation (IMS) is a technique that has been used to increase sensitivity and specificity and to decrease the time required for detection of Salmonella in foods through different methodologies. In this work we report on the development of a method for detection of Salmonella in chicken cuts using in house antibody-sensitized microspheres associated to conventional plating in selective agar (IMS-plating). First, protein A-coated microspheres were sensitized with polyclonal antibodies against lipopolysacharide and flagella from salmonellae and used to standardize a procedure for capturing Salmonella Enteritidis from pure cultures and detection in selective agar. Subsequently, samples of chicken meat experimentally contaminated with S.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Immunomagnetic separation; Rapid methods. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100034 Performance of two ready-to-use systems for enumeration of aerobic mesophilic microorganisms in frozen goat milk BJM Tavolaro,Paula; Ferrati,Analí Ramazotti; Destro,Maria Teresa; Landgraf,Mariza; Franco,Bernadette Dora Gombossy de Melo. Enumeration of aerobic microorganisms in Brazilian pasteurized bovine milk using PetrifilmTM AC and SimplateTM TPC was shown to be influenced by the nature of autochthonous microbiota. This study was conducted to assess if the same occurs in pasteurized goat milk. Pasteurized frozen goat milk (n=62) was purchased in retail stores in São Paulo, SP, Brazil, and submitted to mesophilic aerobic counts using PetrifilmTM AC and SimplateTM TPC, comparing results with those of conventional pour-plates. Correlation indexes above 0.90 were ranked "excellent"; those between 0.80 and 0.89, "good", those from 0.79 to 0.50, "fair", and those below 0.50, "poor". Correlation indexes for PetrifilmTM AC counts were "good", but "poor" using SimplateTM TPC. Comparison of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Rapid methods; Ready-to-use systems; Goat milk; PetrifilmTM AC; SimplateTM TPC. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300017 Registros recuperados: 4 Primeira ... 1 ... Última

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