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	Provedor de dados BJM (2) Autor Davide,Lisete C. (1) Han,Litao (1) Mandlaa,Mandlaa (1) Roca,M. Gabriela (1) Wheals,Alan E. (1) Xu,Hui (1) Yang,Weichao (1) Zhang,Haihong (1) Zhang,Zhongze (1) Mais... Palavra-chave Rapid screening (2) 2-Keto-l-gulonic acid (1) Anthracnose (1) Hygromycin resistance (1) Internal transcribed spacer (1) Ketogulonicigenium vulgare (1) PCR methodology (1) PH indicator (1) Spaceflight mutation (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2017 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A plate method for rapid screening of Ketogulonicigenium vulgare mutants for enhanced 2-keto-l-gulonic acid production BJM Yang,Weichao; Han,Litao; Mandlaa,Mandlaa; Zhang,Haihong; Zhang,Zhongze; Xu,Hui. Abstract A new plate method was developed for rapid screening of Ketogulonicigenium vulgare mutants overproducing 2-keto-l-gulonic acid (2-KLG). The screening methodology took the advantage of the acidity caused by 2-KLG, which changes the color of bromothymol blue (pH indicator) from blue to yellow. Using the proposed method, a mutant, K. vulgare 65, was selected from 20,000 colonies produced by a strain subjected to spaceflight mutagenesis. When co-cultured with Bacillus megaterium 2980 in 20-L fermenters, K. vulgare 65 showed a high conversion rate (94.45%) of l-sorbose to 2-KLG. In contrast to the traditional screening method, this one significantly improved the frequency of obtaining positive mutants. The proposed plate screening method is... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ketogulonicigenium vulgare; PH indicator; Rapid screening; Spaceflight mutation; 2-Keto-l-gulonic acid. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300397 Template preparation for rapid PCR in Colletotrichum lindemuthianum BJM Roca,M. Gabriela; Davide,Lisete C.; Wheals,Alan E.. Isolation of DNA for PCR is time-consuming and involves many reagents. The aim of this work was to optimise a rapid and easy PCR methodology without previous DNA isolation. Different strains of the phytopathogenic fungus Colletotrichum lindemuthianum were used. Protoplasts were generated using lytic enzymes under high incubation temperatures using different methodologies to obtain the template. A rapid (10 minute) methodology was successful for smaller amplicons (<750 bp). Tipo: Info:eu-repo/semantics/article Palavras-chave: Anthracnose; Hygromycin resistance; Internal transcribed spacer; Rapid screening; PCR methodology. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100003 Registros recuperados: 2 Primeira ... 1 ... Última

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