Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados BJID (2) Biol. Res. (1) Autor Arruda,R. S. (1) BURZIO,LUIS O (1) Beck,S. T. (1) Ferreira,A. W. (1) Leite,O. M. (1) MARTÍNEZ,RODRIGO (1) MORALES,CARMEN (1) Miranda,Ana Paula Félix de (1) ROSEMBLATT,MARIO (1) Sato,Neuza Satomi (1) VALENZUELA,PABLO D. T (1) WILHELM,VIVIAN (1) Mais... Palavra-chave Recombinant antigens (3) Diagnosis (1) M. tuberculosis (1) MltB (1) Piscirickettsia salmonis (1) Recombinant vaccines (1) Serological test (1) Syphilis (1) TbpB (1) Treponema pallidum (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Journal article (1) Ano 2008 (1) 2005 (1) 2004 (1) País Brazil (2) Chile (1) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Isolation and expression of the genes coding for the membrane bound transglycosylase B (MltB) and the transferrin binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis Biol. Res. WILHELM,VIVIAN; MORALES,CARMEN; MARTÍNEZ,RODRIGO; ROSEMBLATT,MARIO; BURZIO,LUIS O; VALENZUELA,PABLO D. T. We have isolated and sequenced the genes encoding the membrane bound transglycosylase B (MltB) and the transferring binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis. The results of the sequence revealed two open reading frames that encode proteins with calculated molecular weights of 38,830 and 85,140. The deduced aminoacid sequences of both proteins show a significant homology to the respective protein from phylogenetically related microorganisms. Partial sequences coding the amino and carboxyl regions of MltB and a sequence of 761 base pairs encoding the amino region of TbpB have been expressed in E. coli. The strong humoral response elicited by these proteins in mouse confirmed the immunogenic properties of the recombinant... Tipo: Journal article Palavras-chave: Piscirickettsia salmonis; MltB; TbpB; Recombinant antigens; Recombinant vaccines. Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500008 Combined use of Western blot/ELISA to improve the serological diagnosis of human tuberculosis BJID Beck,S. T.; Leite,O. M.; Arruda,R. S.; Ferreira,A. W.. Two recombinant antigens and a crude bacterial antigen of a wild M. tuberculosis strain were used to detect specific IgG antibodies in sera from 52 patients with pulmonary tuberculosis, confirmed by an acid-fast smear and serum culture of these patients and that of 25 contacts. The patients were not infected with HIV. We evaluated the sensitivity and specificity of ELISA, based on the recombinant TbF6® and TbF6/DPEP antigen and a search for reactivity patterns in the Western blot technique, using whole mycobacterium antigen. Serum samples from 22 healthy individuals and from 30 patients with lung diseases other than tuberculosis were used as controls. The best ELISA results were obtained with the TbF6/DPEP antigen combination, which gave 85% sensitivity... Tipo: Info:eu-repo/semantics/article Palavras-chave: M. tuberculosis; Recombinant antigens; Diagnosis. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000100007 Profile of Anti-Tp47 antibodies in patients with positive serology for syphilis analized by western blot BJID Miranda,Ana Paula Félix de; Sato,Neuza Satomi. In Brazil, syphilis is still a great problem of public health. Serological test is essential for syphilis diagnosis and the current trend is the use of recombinant antigen in the treponemal tests, due to its confirmed higher sensibility and specificity. The purpose of the present study was to analyze the profile of anti-Tp47 antibodies in patients with positive serology for syphilis. One hundred positive sera samples were analyzed by Western Blot (WB) technique, using the recombinant antigen (rTp47). Ten of them did not present antibodies against the fraction rTp47, the results were confirmed by WB using native T. pallidum antigen. All ten samples had antibodies against the fractions Tp17 and Tp15 and presented low reactivity in VDRL, negative results or... Tipo: Info:eu-repo/semantics/article Palavras-chave: Syphilis; Treponema pallidum; Recombinant antigens; Serological test. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000200008 Registros recuperados: 3 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco