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Simulation of population size and genome saturation level for genetic mapping of recombinant inbred lines (RILs) Genet. Mol. Biol.
Silva,Luciano da Costa e; Cruz,Cosme Damião; Moreira,Maurilio Alves; Barros,Everaldo Gonçalves de.
Various population sizes and number of markers have been used to obtain genetic maps. However, the precise number of individuals and markers needed for obtaining reliable maps is not known. We used data simulation to determine the influence of population size, the effect of the degree of marker saturation of the genome, and the number of individuals required for mapping of recombinant inbred lines (RILs). Three genomes with 11 linkage groups were generated with saturation levels of 5, 10 and 20 cM. For each saturation level populations were generated with 50, 100, 154, 200, 300, 500 and 800 individuals with 100 replications for each population size. A total of 2100 populations was generated and mapped. Small marker numbers and small population sizes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Recombinant inbred lines; Molecular markers; Number of individuals; Computer simulation.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600013
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Inheritance pattern and selection criteria for resistance to soybean cyst nematode races 3 and 9 PAB
Cervigni,Gerardo Domingo Lucio; Schuster,Ivan; Sediyama,Carlos Sigueyuki; Barros,Everaldo Gonçalves de; Moreira,Maurilio Alves.
The objective of this work was to determine soybean resistance inheritance to Heterodera glycines Ichinohe (soybean cyst nematode - SCN) races 3 and 9, as well as to evaluate the efficiency of direct and indirect selection in a soybean population of 112 recombinant inbred lines (RIL) derived from the resistant cultivar Hartwig. The experiment was conducted in a completely randomized design, in Londrina, PR, Brazil. The estimated narrow-sense heritabilities for resistance to races 3 and 9 were 80.67 and 77.97%. The genetic correlation coefficient (r g = 0.17; p<0.01) shows that some genetic components of resistance to these two races are inherited together. The greatest genetic gain by indirect selection was obtained to race 9, selecting to race 3 due to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Heterodera glycines; Resistance; SCN; Recombinant inbred lines.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007001000007
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QTL mapping for protein content in soybean cultivated in two tropical environments PAB
Soares,Taís Cristina Bastos; Good-God,Pedro Ivo Vieira; Miranda,Fábio Demolinari de; Soares,Janaína Bastos; Schuster,Ivan; Piovesan,Newton Deniz; Barros,Everaldo Gonçalves de; Moreira,Maurilio Alves.
The objectives of this study were to detect quantitative trait loci (QTL) for protein content in soybean grown in two distinct tropical environments and to build a genetic map for protein content. One hundred eighteen soybean recombinant inbred lines (RIL), obtained from a cross between cultivars BARC 8 and Garimpo, were used. The RIL were cultivated in two distinct Brazilian tropical environments: Cascavel county, in Paraná, and Viçosa county, in Minas Gerais (24º57'S, 53º27'W and 20º45'S, 42º52'W, respectively). Sixty-six SSR primer pairs and 65 RAPD primers were polymorphic and segregated at a 1:1 proportion. Thirty poorly saturated linkage groups were obtained, with 90 markers and 41 nonlinked markers. For the lines cultivated in Cascavel, three QTL...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Linkage map; Molecular markers; Recombinant inbred lines; Quantitative trait loci.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001100012
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Inheritance of resistance to soybean cyst nematode races 3 and 14 in soybean RIL and F2 populations PAB
Silva,Marcia Flores da; Schuster,Ivan; Cervigni,Gerardo Domingo Lucio; Silva,João Flávio Veloso da; Dias,Waldir Pereira; Ferreira,Adésio; Barros,Everaldo Gonçalves de; Moreira,Maurílio Alves.
The objective of this work was to evaluate the soybean inheritance of resistance to cyst nematode races 3 and 14. The following populations where evaluated: one population of recombinant inbred lines (RILs) [Hartwig (resistant) x Y23 (susceptible line)] for races 3, 14 and 9; one population of families F2:3 [M-SOY 8001 (resistant) x MB/BR 46 - Conquista (susceptible)] for race 3; and one population of families F2:3 [(S5995 (resistant) x BRSMG Renascença (susceptible)] for race 14. In RIL populations, four epistatic genes were identified which conditioned resistance to race 14, and three epistatic ones for resistance to races 3 and 9. The lack of one gene provided moderate resistance under all situations. The highest number of genes for resistance to race...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Heterodera glycines; Improvement; Recombinant inbred lines; Soybean cyst nematode.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007001200010
Registros recuperados: 4
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