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| Thirumalaiandi,Ramasubramanian; Selvaraj,Michael Gomez; Rajasekaran,Raghu; Subbarayalu,Mohankumar. |
| Genomic DNA sequences sharing homology with NBS region of resistance gene analogs were isolated and characterized from Pongamia glabra, Adenanthera pavonina, Clitoria ternatea and Solanum trilobatum using PCR based approach with primers designed from conserved regions of NBS domain. The presence of consensus motifs viz., kinase 1a, kinase 2, kinase 3a and hydrophobic domain provided evidence that the cloned sequences may belong to the NBS-LRR gene family. Conservation of tryptophan as the last residue of kinase-2 motif further confirms their position in non-TIR NBS-LRR family of resistance genes. The Resistance Gene Analogs (RGAs) cloned from P. glabra, A. pavonina, C. ternatea and S. trilobatum clustered together with well- characterized non-TIR-NBS-LRR... |
| Tipo: Journal article |
Palavras-chave: Adenanthera pavonina; Clitoria ternatea; NBS-LRR; Pongamia glabra; Resistance gene analogs; Solanum trilobatum. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400004 |
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| Almeida,Dênia Pires de; Castro,Isabel Samila Lima; Mendes,Tiago Antônio de Oliveira; Alves,Danúbia Rodrigues; Barka,Geleta Dugassa; Barreiros,Pedro Ricardo Rossi Marques; Zambolim,Laércio; Sakiyama,Ney Sussumu; Caixeta,Eveline Teixeira. |
| ABSTRACT: The biotrophic fungus Hemileia vastatrix causes coffee leaf rust (CLR), one of the most devastating diseases in Coffea arabica . Coffee, like other plants, has developed effective mechanisms to recognize and respond to infections caused by pathogens. Plant resistance gene analogs (RGAs) have been identified in certain plants as candidates for resistance ( R ) genes or membrane receptors that activate the R genes. The RGAs identified in different plants possess conserved domains that play specific roles in the fight against pathogens. Despite the importance of RGAs, in coffee plants these genes and other molecular mechanisms of disease resistance are still unknown. This study aimed to sequence and characterize candidate genes from coffee plants... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea arabica; Coffee leaf rust; Resistance gene analogs; Molecular markers; Plant breeding. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162021000601101 |
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