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Optimization of some processing conditions for Kokoro production using Response Surface Methodology 111
Idowu, Atinuke Olamide; Aworh, Charles Ogugua.
Abstract: Deep fat frying of kokoro was investigated with the aim of upgrading the traditional production process by optimizing the processing conditions and improving its nutritional value by incorporation of African yam bean (AYB), an underutilized crop with high nutritional value.  The effect of frying temperature, frying time and quantity of AYB flour on moisture, oil contents and crispness of the kokoro was evaluated by Box Behnken experimental design of Response Surface Methodology.  Optimization was achieved by maximizing crispness and minimizing moisture and oil contents.  Statistical analysis with response regression showed that breaking force, oil and moisture contents were significantly (p<0.05) correlated with frying temperature and time....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Food Science; Food processing and preservation; Food tourism and hospitality management Maize; African yam bean; Response surface methodology; Deep fat frying; Optimum processing condition; Kokoro.
Ano: 2014 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/2819
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Enzymatic clarification of carrot juice by using response surface methodology 111
Alam, Md. Shafiq; Ahuja, Geetika; Gupta, Kalika.
 Carrot juice was treated with pectinase at various enzyme concentrations (0.01% to 0.1%), process temperatures (350C to 550C) and incubation time (40 to 120 min).  The effect of these enzyme treatments on filterability, clarity, turbidity and viscosity of the juice were studied by employing a second order central composite design.  The coefficient of determination (R2) values for filterability, clarity, turbidity and viscosity were greater than 0.85.  Statistical analysis showed that filterability, clarity, viscosity and turbidity were significantly correlated to enzyme concentration, incubation temperature and incubation time.  Enzyme concentration was the most important factor affecting the characteristics of the carrot juice as it exerted a highly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Processing and Food Engineering; Post Harvest Tecnology carrot juice; Enzyme clarification; Response surface methodology.
Ano: 2014 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/2804
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Prediction Of Lateral Force In An Off-Road Tire As A Function Of Seven Variables Using Response Surface Methodology (RSM) 111
Abdolmaleki, Hamid; Jafari, Ali; Mousazadeh, Hossein; Hajiahmad, Ali.
In this study an in-situ Single Wheel Tester (iSWT) was used to investigate the effect of some of the most important independent variables on the lateral force of a high lug agricultural tire. The variables include some soil parameters and tire characteristics. Response Surface Methodology (RSM) also was used to construct a design of experiment and propose the lateral force model as a function of seven variables (various combination treatments of soil characteristics and tire parameters). A total of 152 experimental tests were conducted to find appropriate model. Reduced form of a quadratic model was selected to predict the tire lateral force (R2 > 0.9). The results show that the side slip angle and moisture content had major and minimal effect on tire...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Response surface methodology; Single wheel tester; Side slip angle; Normal load; Drawbar pull; Moisture content; Tire inflation pressure.
Ano: 2018 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/4776
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Extraction of bioactive compounds and free radical scavenging activity of purple basil (Ocimum basilicum L.) leaf extracts as affected by temperature and time 42
PEDRO,ALESSANDRA C.; MOREIRA,FERNANDA; GRANATO,DANIEL; ROSSO,NEIVA D..
In the current study, response surface methodology (RSM) was used to assess the effects of extraction time and temperature on the content of bioactive compounds and antioxidant activity of purple basil leaf (Ocimum basilicum L.) extracts. The stability of anthocyanins in relation to temperature, light and copigmentation was also studied. The highest anthocyanin content was 67.40 mg/100 g extracted at 30 °C and 60 min. The degradation of anthocyanins with varying temperatures and in the presence of light followed a first-order kinetics and the activation energy was 44.95 kJ/mol. All the extracts exposed to light showed similar half-lives. The extracts protected from light, in the presence of copigments, showed an increase in half-life from 152.67 h for the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anthocyanins; Extraction; Pigments; Response surface methodology.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000301055
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Enhanced Production Of Streptokinase By Chemical Mutagenesis Of Streptococcus agalactiae EBL-20 52
Arshad,Arooj; Zia,Muhammad Anjum; Asghar,Muhammad; Joyia,Faiz Ahmad.
Abstract Streptokinase (SK) is an enzyme that is used for the treatment of cardiovascular diseases. The current study focused on the enhanced production of SK by inducing mutation in Streptococcus agalactiae EBL-20 and optimization of medium components and culture conditions for the maximum growth of mutant derived strain. S. agalactiae EBL-32 was selected as a potent mutant after exposure of S. agalactiae EBL-20 to EMS for 180 minutes. SK activity obtained from mutant derived strain was found to be 1.6 fold higher as compared to the activity achieved by wild strain. Nutritional requirements of the mutated strain were optimized by single factor analysis method suggesting glucose as the optimum carbon source; yeast extract and peptone as a suitable nitrogen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethyl methanesulfonate; Fermentation; Response surface methodology; Streptokinase.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100200
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Statistical Modelling and Optimization of Fermentation Medium for Lincomycin Production by Streptomyces lincolnensis Immobilized Cells 52
Abdelwahed,Nayera A.M.; Gomaa,Eman Zakaria; Hassan,Amira A..
ABSTRACT Response surface methodology was used to optimize lincomycin production by Streptomyces lincolnensis NRRL ISP-5355 in submerged fermentation. Screening of fermentation medium components to find their relative effect on lincomycin production was done using Plackett-Burman design. Malt extract, dextrin, soluble starch and (NH4)2SO4 were the most significant nutrient influenced on lincomycin production. Central composite design was applied to determine optimal concentrations of these factors and the effect of their mutual interactions. The interaction between soluble starch and (NH4)2SO4 was found to enhance the production, whereas malt extract and dextrin exhibited an influence independent from the other two factors. Using this statistical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Optimization; Lincomycin; Streptomyces lincolnensis; Response surface methodology; Immobilization.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100405
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Synergism between dietary vitamin E and exogenous phytic acid in prevention of warmed-over flavour development in chicken breast meat, Pectoralis major M. 52
Soares,Adriana Lourenço; Olivo,Rubison; Shimokomaki,Massami; Ida,Elza Iouko.
The effect of alpha-tocopheryl acetate (AT) supplementation and exogenous application of this vitamin E associated with phytic acid (PA) on chicken breast meat WOF development was assessed. Control group was fed with 7.7IU of AT/kg of ration and supplemented group was fed with 200.0IU of AT/kg of ration. Dietary vitamin E as measured by TBARS inhibited WOF development by 78.9; 69.0; 60.7 and 46.5% (p<0.05) during storage at 6° C for 0, 1, 3 and 5 days, respectively. This inhibition was significantly increased (p<0.05) by 86.1; 91.6; 92.9 and 95.3% during storage at 6° C for 0, 1, 3 and 5 days, respectively, when 2mM PA was added in supplemented breast meat. In the exogenous experiment, through Response Surface Methodology design it was found out AT...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Warmed over flavour; Vitamin E; Phytic acid; Lipid oxidation inhibition; Response surface methodology; Breast poultry meat.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100008
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Immobilization of alpha-amylase produced by Bacillus circulans GRS 313 52
Dey,Gargi; Bhupinder,Singh; Banerjee,Rintu.
A maltooligosaccharide-forming amylase from B circulans GRS 313 was immobilized by entrapment in calcium alginate beads. The immobilized activity was affected by the size of the bead and bead size of 2mm was found to be most effective for hydrolysis. Kinetics constants, Km and Vmax were estimated and were found to be affected by the bead size. The catalytic activity of the enzyme was studied in presence of various starchy residues and metal ions. HgCl2, CuSO4 and FeCl3 caused inhibition of the enzyme. The reaction conditions, pH and temperature, was optimized using response surface methodology. At the optimum pH and temperature of 4.9 and 57ºC, the apparent activity was 25.6U/g of beads, resulting in almost 2-fold increase in activity. The immobilized...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus circulans GRS313; Entrapment; Maltooligosaccharide-forming amylase; Response surface methodology; Starchy residues.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200005
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Production of pectinases by A. niger: influence of fermentation conditions 52
Martos,María A.; Martinez Vazquez,Francisco; Benassi,Fernando O.; Hours,Roque A..
Response surface methodology was used for optimization of polygalacturonase (PG) and pectinesterase (PE) production in submerged fermentation by A.niger. A Central Composite Experimental Design was applied, consisting of 22 experiments, including eight central points. Variables studied were: fermentation time (24 to 120 h), pH (3.5 to 6.5) and initial concentration of pectin (5 to 20 g/l). Maximum PE production was 220 U/l, after 74 h of culture, in a medium containing 20 g/l of pectin (pH 6.5). The optimal conditions for PG production were pH: 4.1, 20 g/l of pectin and 94 h of fermentation with a maximum value of 1032 U/l. Under these conditions, the PE production was low (15 U/l). A liquid extract with high PG activity and low PE activity could be...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pectinases; Polygalacturonase; Pectinesterase; Aspergillus niger; Response surface methodology; Citrus pectin.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000300007
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Optimization of thermostable α- amylase production by Streptomyces erumpens MTCC 7317 in solid-state fermentation using cassava fibrous residue 52
Shaktimay,Kar; Datta,Tapan Kumar; Ray,Ramesh Chandra.
Production of α- amylase under solid state fermentation by Streptomyces erumpens MTCC 7317 was investigated using cassava fibrous residue, one of the major solid waste released during extraction of starch from cassava (Manihot esculenta Crantz). Response surface methodology (RSM) was used to evaluate the effect of the main variables, i.e., incubation period (60 h), moisture holding capacity (60%) and temperature (50(0)C) on enzyme production by applying a full factorial Central Composite Design. Varying the inoculum concentration (5-25%) of S. erumpens showed that 15% inoculum (v/w, 2.5 x 10(6) CFU/ml) was the optimum for α- amylase production. Among the different nitrogen sources supplemented, beef extract was most suitable for enzyme production. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Α- amylase; Cassava fibrous residue; Optimization; Response surface methodology; Solid state fermentation.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200008
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Enhanced exoinulinase production from Kluyveromyces marxianus YS-1 using response surface methodology 52
Singh,Ram Sarup; Lotey,Shveta.
Optimization of critical medium components for exoinulinase production by Kluyveromyces marxianus YS-1 at shake-flask was investigated using response surface methodology (RSM) based on a central composite rotatable design (CCRD). A five-level with five factors CCRD was used to evaluate the influence of related factors including concentration of inulin, meat extract, calcium chloride, sodium dodecyl sulphate and medium pH. Optimum values obtained by RSM were 2% inulin, 2.17% meat extract, 0.65 mM calcium chloride, 0.10 mM sodium dodecyl sulphate and pH 5.5. Optimized medium projected a theoretical exoinulinase production of 63.61 IU/mL and biomass yield of 0.965 (OD600/10). Multiple correlation coefficient R was 0.9976 and 0.9605 for exoinulinase production...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Kluyveromyces marxianus; Exoinulinase; Medium optimization; Central composite rotatable design; Response surface methodology.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500002
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Enhanced lipase recovery through RSM integrated differential evolutionary approach from the fermented biomass 52
Garlapati,Vijay Kumar; Vundavilli,Pandu Ranga; Banerjee,Rintu.
The aim of this work was to apply a modeling integrated optimisation approach for a complex, highly nonlinear system for an extracellular lipase extraction process. The model was developed using mutation, crossover and selection variables of Differential Evolution (DE) based on central composite design of Response Surface Methodology. The experimentally validated model was optimized by DE, a robust evolutionary optimization tool. A maximum lipase activity of 134.13 U/gds (more than 36.28 U/gds compared to one variable at a time approach) was observed with the DE-stated optimum values of 25.01% dimethyl sulfoxide concentration, 40 mM buffer, 128.52 min soaking time and 35ºC with the DE control parameters, namely number of population, generations, crossover...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Modeling; Optimization; Lipase extraction; Response surface methodology; Differential evolution; Solid state fermentation.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500001
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Response surface analysis on the effect of temperature and pH on Growth and proteolytic activity of thermophilic Bacillus sp 52
Gorlach-Lira,Krystyna; Pedroza,Maria Lourdes Vieira; Burdziej-Pokojska,Aleksandra; Rozycki,Henryk; Dahm,Hanna.
Proteolytic activity and cell biomass of thermophilic Bacillus sp. strain were evaluated at various levels of initial pH and temperature by applying response surface methodology. The mineral medium containing yeast extract (0.01%) and starch (1%) was used throughout the experiment. The results of statistical analysis revealed the polynomial model with high coefficient of determination (R² = 0.8) for the biomass and total proteolytic activity of the strain studied. This model showed a satisfactory adjustment of the statistic model with the experimental data. The p values showed that the temperature and pH had significant effect on biomass and proteolytic activity (P<0.05) of strain tested. The highest proteolytic activity (2.333 U/ml/h) of the Bacillus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus; Proteolytic activity; Response surface methodology.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500009
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Optimization of Endoglucanase Production from a Novel Bacterial Isolate, Arthrobacter sp. HPG166 and Characterization of Its Properties 52
Huang,Shengwei; Deng,Guanjun; Yang,Ying; Wu,Zhengyan; Wu,Lifang.
ABSTRACTIn this study, a potential novel cellulolytic bacteriumArthrobacter sp. HPG166 was isolated from the hindgut of root-feeding larvaeHolotrichia parallela. Optimization of fermentation factors for endoglucanase production byArthrobacter sp. HPG166 was carried out via response surface methodology. Sodium carboxymethylcellulose 1.19% (w/v) and beef extract 0.35% (w/v) were the ideal combination of carbon and nitrogen sources for enzyme production; the optimum temperature and pH for cellulase production were 34°C and pH 8.0 respectively. Under the optimized fermentation conditions, the maximum endoglucanase activity of 1.411 U mL-1 was obtained. The crude endoglucanase was thermotolerant as it retained 50.31% of its activity after incubation at 70°C for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Scarab larvae; Cellulolytic bacteria; Response surface methodology; Thermotolerant cellulases; Effects of Metal ions.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500692
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Optimization Medium Composition for Vitamin K2 by Flavobacterium sp. using Response Surface Methodology and Addition of Arachis hypogaea 52
Tan,Mu; Liu,Hui; Li,Zhemin; Sun,Xiaowen; Zheng,Zhiming; Zhao,Genhai.
ABSTRACT The purpose of this research was to enhance the production of vitamin K2 by fermentation optimization and Arachis hypogaea supplementation in Flavobacterium sp. mutant SP-L-01. Optimized culture condition were as follows: 6-days shake-flask culture at 37oC with initial pH value 7.0 ± 0.2, shaking speed in 120 r/min and medium volume of 30 mL with 2% inoculums. After optimization of fermentation medium by response surface methodology (RSM), optimized medium were maltose 23.8 g/l, glucose 9.69 g/l, beef extract 15 g/l, K2HPO4 4.5 g/l,NaCl 3.0 g/l and MgSO4·7H2O 0.3 g/l. Production of vitamin K2 after optimization reached to 10.97 mg/l, which is 79.25% higher than that before optimization (6.12 mg/l). 3 mg/mL of arachis hypogaea was added into the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arachis hypogaea; D-(+)-catechin; Response surface methodology; Vitamin K2.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100501
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Optimization of process parameters for cellulase production from Bacillus sp. JS14 in solid substrate fermentation using response surface methodology 52
Singh,Jagdish; Kaur,Pawandeep.
The aim of this work was to isolate the potent bacterial strains for the production of cellulose enzyme. A total 30 bacterial isolates showed positive results for the cellulase production but highest enzyme activity was shown by isolate JS 14. From the morphological and biochemical reactions, the isolate was identified as Bacillus sp. Cellulase production was studied by this strain using response surface methodology (RSM). A central composite design (CCD) quadratic response surface was applied to explicate the parameters that significantly affected cellulase production in solid substrate fermentation (SSF). The wheat bran concentration and incubation period were significant factors. The process parameters optimized with response surface methodology was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cellulase; Response surface methodology; Optimization.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000400004
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Rheological properties of emulsions stabilized by green banana (Musa cavendishii) pulp fitted by power law model 52
Izidoro,Dayane Rosalyn; Scheer,Agnes de Paula; Sierakowsk,Maria-Rita.
In this work, the rheological behaviour of emulsions (mayonnaises) stabilized by green banana pulp using the response surface methodology was studied. In addition, the emulsions stability was investigated. Five formulations were developed, according to design for constrained surfaces and mixtures, with the proportion, respectively: water/soy oil/green banana pulp: F1 (0.10/0.20/0.70), F2 (0.20/0.20/0.60), F3 (0.10/0.25/0.65), F4 (0.20/0.25/0.55) and F5 (0.15/0.225/0.625) .Emulsions rheological properties were performed with a rotational Haake Rheostress 600 rheometer and a cone and plate geometry sensor (60-mm diameter, 2º cone angle), using a gap distance of 1mm. The emulsions showed pseudoplastic behaviour and were adequately described by the Power Law...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Green banana pulp; Emulsion; Rheology; Response surface methodology.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600026
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Screening and optimization of low-cost medium for Pseudomonas putida Rs-198 culture using RSM 58
Peng,Yanjie; He,Yanhui; Wu,Zhansheng; Lu,Jianjiang; Li,Chun.
The plant growth-promoting rhizobacterial strain Pseudomonas putida Rs-198 was isolated from salinized soils from Xinjiang Province. We optimized the composition of the low-cost medium of P. putida Rs-198 based on its bacterial concentration, as well as its phosphate-dissolving and indole acetic acid (IAA)-producing capabilities using the response surface methodology (RSM), and a mathematical model was developed to show the effect of each medium component and its interactions on phosphate dissolution and IAA production. The model predicted a maximum phosphate concentration in medium containing 63.23 mg/L inorganic phosphate with 49.22 g/L corn flour, 14.63 g/L soybean meal, 2.03 g/L K2HPO4, 0.19 g/L MnSO4 and 5.00 g/L NaCl. The maximum IAA concentration...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas putida Rs-198; Low-cost medium; Phosphorus-dissolving; IAA; Response surface methodology.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400013
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Optimization of growth and bacteriocin production by Lactobacillus sakei subsp. sakei2a 58
Malheiros,Patrícia S.; Sant’Anna,Voltaire; Todorov,Svetoslav D.; Franco,Bernadette D.G.M..
Lactobacillus sakei subsp. sakei 2a is a bacteriocinogenic lactic acid bacterium isolated from Brazilian pork sausage, capable of inhibiting the growth of microbial pathogens, mainly Listeria monocytogenes. In order to optimize bacteriocin production for industrial applications, this study evaluated the effect of supplementation of MRS broth with glucose, Tween 20, Tween 80, sodium citrate, potassium chloride and cysteine, and effect of the initial pH and temperature of incubation of the medium on production of bacteriocins by L. sakei 2a. Adding glucose and Tween 20 to the medium, an initial pH of 5.0 or 5.5, and incubation temperatures of 25 °C or 30 °C resulted to the highest bacteriocin yields. Thus, a 24 factorial design with the four variables was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactobacillus sakei; Bacteriocin; Response surface methodology; Optimization.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300825
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Production of angkak through co-culture of Monascus purpureus and Monascus ruber 58
Panda,Bibhu Prasad; Javed,Saleem; Ali,Mohd.
Angkak (red mold rice, red yeast rice, Chinese red rice) is a traditional Chinese medicine produced by solid-state fermentation of cooked non-glutinous rice with Monascus species. The secondary metabolite of Monascus species, monacolin K /lovastatin, has been proven to lower blood lipid levels. In this study, a co-culture of Monascus purpureus MTCC 369 and Monascus ruber MTCC 1880 was used for angkak production. Four medium parameters screened by Plackett-Burman design were optimized by response surface methodology for highest lovastatin production in angkak during solid-state fermentation by the co-culture. Maximum lovastatin production of 2.84 mg g-1 was predicted in solid medium containing 20 g rice and 40 ml liquid nutrients medium (malt extract 9.68 g...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Angkak. Co-culture; Monascus purpureus; Monascus ruber; Lovastatin; Response surface methodology.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300028
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