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	Provedor de dados BABT (1) BJM (1) Genet. Mol. Biol. (1) Neotropical Entomology (1) Autor Araújo,Elza Fernandes de (1) Arias,Maria C. (1) Asano,Karen Miyuki (1) Barros,Iracema Nunes de (1) Brandão,Paulo Eduardo (1) Lima,Juliana Oliveira (1) Lopes,Francis Julio Fagundes (1) Moretto,Geraldo (1) Queiroz,Marisa Vieira de (1) Richtzenhain,Leonardo José (1) Sandri,Thaisa Lucas (1) Sayed-Tabatabaei,Badraldin Ebrahim (1) Shahinnia,Fahimeh (1) Silva,Viviane Aline Oliveira (1) Souza,Sibele Pinheiro de (1) Mais... Palavra-chave Restriction enzyme (4) Barley (Hordeum vulgare L.) (1) Bovine coronavirus (1) Gene S (1) Genome sequencing (1) Genotypes (1) Mismatched primer (1) Moniliophthora perniciosa (1) MtDNA (1) PCR+RLFP (1) Polymorphism (1) SNP genotyping (1) Transformation (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Ano 2010 (1) 2009 (1) 2008 (1) 2005 (1) País Brazil (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Restriction enzyme improves the efficiency of genetic transformations in Moniliophthora perniciosa, the causal agent of witches’ broom disease in Theobroma cacao BABT Lopes,Francis Julio Fagundes; Queiroz,Marisa Vieira de; Lima,Juliana Oliveira; Silva,Viviane Aline Oliveira; Araújo,Elza Fernandes de. The presence of restriction enzymes in the transformation mixture improved the efficiency of transformation in Moniliophthora perniciosa. The influence of the vector shape (linear or circular), the patterns of plasmid integration in genomic sites and the influence of the promoter used to express the gene marker were also analyzed. The addition of BamHI or NotI increased the number of transformants by 3-10-fold and 3-fold, respectively, over the control without added enzyme. The use of pre-linearized plasmid did not increase the transformation efficiency in comparison with the circular plasmid. However, the frequency of multi-copy transformants increased significantly. The transformation procedure here reported resulted in better production of protoplasts... Tipo: Info:eu-repo/semantics/article Palavras-chave: Moniliophthora perniciosa; Transformation; Restriction enzyme. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100004 Differentiation of Bovine coronavirus (BCoV) genotypes by a restriction enzyme assay BJM Souza,Sibele Pinheiro de; Asano,Karen Miyuki; Sandri,Thaisa Lucas; Barros,Iracema Nunes de; Richtzenhain,Leonardo José; Brandão,Paulo Eduardo. This article reports the use of the GsuI restriction enzyme to differentiate genotypes of Bovine Coronavirus (BCoV), based on an 18-nucleotide deletion of S1-coding region found in one of the two genotypes. It was concluded that this assay can be used as a rapid tool for BCoV genotypes differentiation. Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine coronavirus; Restriction enzyme; Genotypes; Gene S. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300034 Conversion of barley SNPs into PCR-based markers using dCAPS method Genet. Mol. Biol. Shahinnia,Fahimeh; Sayed-Tabatabaei,Badraldin Ebrahim. Molecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome. In combination with a PCR assay, the corresponding SNP can be analyzed as a derived cleaved amplified polymorphic sequence (dCAPS) marker. The dCAPS method exploits the well-known specificity of a restriction endonuclease for its recognition site and can be used to virtually detect any SNP. Here, we describe the use of the dCAPS method for detecting single-nucleotide changes by means of a barley EST, CK569932, PCR-based marker. Tipo: Info:eu-repo/semantics/article Palavras-chave: Barley (Hordeum vulgare L.); Genome sequencing; Mismatched primer; Restriction enzyme; SNP genotyping. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300020 Detection of mitochondrial DNA restriction site differences between the subspecies of Melipona quadrifasciata Lepeletier (Hymenoptera: Apidae: Meliponini) Neotropical Entomology Moretto,Geraldo; Arias,Maria C.. The endemic range of the stingless bee Melipona quadrifasciata Lepeletier extends from the southern most state in Brazil, Rio Grande do Sul, up to the state of Paraíba, where it originally inhabited the Atlantic Rain Forest. Two subspecies have been morphologically recognized based on the tergal band pattern (yellow stripes across the abdomen), M. q. quadrifasciata and M. q. anthidioides. The objective of the present study was to characterize restriction site variation in the mitochondrial DNA of each subspecies. Four colonies of each subspecies were sampled. One individual per colony was used for DNA extraction and further analysis through amplification of nine fragments of the mitochondrial genome and digestion with 13 restriction enzymes (PCR+RFLP).... Tipo: Info:eu-repo/semantics/article Palavras-chave: Restriction enzyme; Polymorphism; PCR+RLFP; MtDNA. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2005000300004 Registros recuperados: 4 Primeira ... 1 ... Última

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