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	Provedor de dados BJID (2) Autor Boeira,Thaís da Rocha (1) Carazzolle,Marcelo Falsarella (1) Coser,Janaina (1) Fonseca,André Salvador Kazantzi (1) Ikuta,Nilo (1) Leite,Domingos da Silva (1) Lunge,Vagner Ricardo (1) Moura,Claúdia de (1) Tiba,Monique Ribeiro (1) Mais... Palavra-chave Restriction fragment length (2) Antigens (1) Bacterial (1) DNA probes (1) Escherichia coli (1) HPV polymerase chain reaction polymorphism (1) Polymerase chain reaction (1) Polymorphism (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2011 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Human papillomavirus detection and typing using a nested-PCR-RFLP assay BJID Coser,Janaina; Boeira,Thaís da Rocha; Fonseca,André Salvador Kazantzi; Ikuta,Nilo; Lunge,Vagner Ricardo. BACKGROUND: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to detect and type HPV based on the analysis of L1 gene. METHODS: Analysis of published DNA sequence of mucosal HPV types to select sequences of new primers. Design of an original nested-PCR assay using the new primers pair selected and classical MY09/11 primers. HPV detection and typing in cervical samples using the nested-PCR-RFLP assay. RESULTS: The nested-PCR-RFLP assay detected and typed HPV in cervical samples. Of the total of 128 clinical samples submitted to simple PCR and nested-PCR for detection of HPV,... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA probes; HPV polymerase chain reaction polymorphism; Restriction fragment length. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500009 Identification of putative new Escherichia coli flagellar antigens from human origin using serology, PCR-RFlP and DNA sequencing methods BJID Tiba,Monique Ribeiro; Moura,Claúdia de; Carazzolle,Marcelo Falsarella; Leite,Domingos da Silva. Escherichia coli has been isolated frequently, showing flagellar antigens that are not recognized by any of the 53 antisera, provided by the most important reference center of E. coli, The International Escherichia and Klebsiella Center (WHO) of the Statens Serum Institute, Copenhagen, Denmark. The objective of this study was to characterize flagellar antigens of E. coli that express non-typeable H antigens. The methods used were serology, PCR-RFLP and DNA sequencing. This characterization was performed by gene amplification of the fliC (flagellin protein) by polymerase chain reaction in all 53 standards E.coli strains for the H antigens and 20 E. coli strains for which the H antigen was untypeable. The amplicons were digested by restriction enzymes, and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Escherichia coli; Antigens; Bacterial; Polymerase chain reaction; Polymorphism; Restriction fragment length. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200009 Registros recuperados: 2 Primeira ... 1 ... Última

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