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Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum BJM
Cardoso,Patrícia Gomes; Queiroz,Marisa Vieira de; Pereira,Olinto Liparini; Araújo,Elza Fernandes de.
Two species from the genus Penicillium, Penicillium expansum and P. griseoroseum (Brasilian isolates) were characterized morphologic and molecularlly. Morphological variability was detected among isolates in regard to colony morphology and to conidia coloration. The molecular characterization was based on the RAPD markers, telomeric fingerprinting and ITS sequencing. A total of 78 RAPD primers were used and 8 presented differences in band patterns with 54% of the amplified polymorphic fragments. The monomorphic fragments of 600 bp (P. expansum) and 594 bp (P. griseoroseum) were amplified. The only internal transcribed spacer region variation detected between the two species was the additional six initial nucleotides. The analysis by telomeric...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ribossomal DNA; Internal Transcribed Spacer; Subtelomeric Region; Penicillium spp.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100015
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