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Loss-Morais,Guilherme; Turchetto-Zolet,Andreia Carina; Etges,Matheus; Cagliari,Alexandro; Körbes,Ana Paula; Maraschin,Felipe dos Santos; Margis-Pinheiro,Márcia; Margis,Rogério. |
Ribosome-inactivating proteins (RIPs) are enzymes that inhibit protein synthesis after depurination of a specific adenine in rRNA. The RIP family members are classified as type I RIPs that contain an RNA-N-glycosidase domain and type II RIPs that contain a lectin domain (B chain) in addition to the glycosidase domain (A chain). In this work, we identified 30 new plant RIPs and characterized 18 Ricinus communis RIPs. Phylogenetic and functional divergence analyses indicated that the emergence of type I and II RIPs probably occurred before the monocot/eudicot split. We also report the expression profiles of 18 castor bean genes, including those for ricin and agglutinin, in five seed stages as assessed by quantitative PCR. Ricin and agglutinin were the most... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agglutinin; Evolution; Lipase; Ricinus communis; Ricin; RIPs. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100011 |
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Cobianchi,Juliana Variz; Oliveira,André Soares de; Campos,José Maurício de Souza; Guimarães,Amanda Vasconcelos; Valadares Filho,Sebastião de Campos; Cobianchi,Flávio Pereira; Oliveira,Thalita Evani Silva de. |
The effect of replacing of 0; 0.33; 0.67 and 1.0 (kg/kg) of soybean meal (SBM) by undecorticated castor seed meal treated with calcium oxide (CMT - 60 g/kg) was evaluated on performance and efficiency of nutrient utilization in dairy cows. Sixteen Holstein and crossbred cows were distributed in four 4 × 4 latin squares. Animals received concentrated feed at a ratio of 1 kg for 3 kg of milk produced, in the natural matter. The diets had the same amount of nitrogen (150.4 g crude protein/kg DM), containing 325.6 g of concentrated feed/kg DM. There was no effect on the serum concentration of transaminase and the animals showed no clinical symptoms of intoxication by ricin. The intake of DM, crude protein (CP) and non-fibrous carbohydrates (NFC) reduced from... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Digestibility; Intake; Milk production; Nitrogenous compounds; Ricin; Ricinus communis L.. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001000015 |
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Furtado,Rafael Nogueira; Pompeu,Roberto Cláudio Fernandes Franco; Cândido,Magno José Duarte; Pereira,Elzânia Sales; Lopes,Marcos Neves; Rogério,Marcos Cláudio Pinheiro. |
ABSTRACT The effect of substituting soybean meal (SM) with four levels (0, 33, 67 and 100%) of detoxified castor cake (DCC) on intake, nutrient digestibility and nitrogen balance was evaluated in uncastrated, male, crossbred sheep in a randomised complete block design with four treatments and five replications. There was a decreasing linear effect from the levels of substitution on dry matter (DM), organic matter (OM) and crude protein (CP) intake, total carbohydrates (TC), non-fibrous carbohydrates (NFC) and total digestible nutrients (TDN), and on DM, OM and TC digestibility, for reductions of 2.04, 1.70, 0.35, 1.56, 1.89 and 1.67 g dia-1 and 0.34, 0.53 and 0.51 g kg-1 DM respectively, for each 1% SM substituted with DCC. DMI and NDFap expressed, in %BW... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biodiesel; Detoxification; Ricin; Ricinus communis; Nutritional value.. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000100412 |
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Fernandes,K.V.; Deus-de-Oliveira,N.; Godoy,M.G.; Guimarães,Z.A.S.; Nascimento,V.V.; Melo,E.J.T. de; Freire,D.M.G.; Dansa-Petretski,M.; Machado,O.L.T.. |
Ricinus communis L. is of great economic importance due to the oil extracted from its seeds. Castor oil has been used for pharmaceutical and industrial applications, as a lubricant or coating agent, as a component of plastic products, as a fungicide or in the synthesis of biodiesel fuels. After oil extraction, a castor cake with a large amount of protein is obtained. However, this by-product cannot be used as animal feed due to the presence of toxic (ricin) and allergenic (2S albumin) proteins. Here, we propose two processes for detoxification and allergen inactivation of the castor cake. In addition, we establish a biological test to detect ricin and validate these detoxification processes. In this test, Vero cells were treated with ricin, and cell death... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ricinus communis; 2S albumin; Ricin; Biodiesel fuel; Solid-state fermentation; Vero cell. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100002 |
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