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Germination and germ tube growth of the arbuscular mycorrhizal fungi Gigaspora albida in different substrates BJM
Maia,Leonor C.; Yano-Melo,Adriana M..
The role of the substrate on germination of Gigaspora albida Schenck & Smith was investigated. Spores were desinfested with 0.5% sodium hypochlorite (20 min.) and placed on Petri dishes over a Millipore filter, with one of the following media: a- 1% water-agar; b- water-agar + aqueous extract of roots of Panicum miliaceum L.; c- salt medium of Murashige & Skoog (MS) or sterilized sand; and incubated in the dark at room temperature (28ºC ± 2). The experimental design was at random with four treatments and four replicates. Germination was evaluated every 7 days until the 28th day. The water-agar medium was the most feasible for spore germination at the 7th day, followed by the MS medium at the 14th day. Conversely, the sand and the root...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Water-agar; MS medium; Root extract; Glomales; Panicum miliaceum.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400005
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