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| Registros recuperados: 39 | |
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| Simaluiza-Masabanda,Rosa Janneth; Cevallos-Solórzano,Gabriela; Bourdett-Stanziola,Lurys; Arévalo-Jaramillo,Paulina; Ruiz-Ramón,Jeimmy; Villacis-Villa,Diana; Torres-Bailón,Paula C; Fernández Jaramillo,Heriberto. |
| Fueron estudiadas, para la búsqueda de rotavirus, 341 muestras fecales obtenidas de sendos niños menores de cinco años con diarrea aguda, atendidos en dos centros de salud de la ciudad de Loja - Ecuador. De ellas, 56 (16%) fueron positivas para rotavirus, siendo genotipificadas 33 muestras para la determinación de genotipos G y P. Los genotipos más frecuentes fueron G4 (42%), P[6] (36%) y la combinación mixta G4+9P[6] (21%). Este es el primer estudio de carácter molecular en rotavirus realizado en el sur de Ecuador y los datos obtenidos ratifican la variabilidad de cepas de rotavirus circulantes en este país. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Genotipos; Ecuador. |
| Ano: 2016 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572016000400015 |
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| Lonien,S.C.H.; Bolognini,A.M.; Linhares,R.E.C.; Nozawa,C.M.. |
| A citopatologia in vitro de uma cepa de rotavírus porcino adaptado em cultura de células foi comparada à estirpe-protótipo símia (SA-11). O efeito citopático (ECP) produzido pelos vírus foi semelhante embora a estirpe porcina tivesse apresentado algumas alterações diferentes, como o acentuado estreitamento do citoplasma, com grande perda do volume citoplasmático. O vírus porcino apresentou menor número de plaques de ECP porém com diâmetro maior em relação ao vírus símio, demonstrando maior capacidade de disseminação célula-célula, quase oito vezes mais, a julgar pelo diâmetro dos plaques de ECP. Os elementos do citoesqueleto das células infectadas revelaram uma reorganização semelhante para ambas as estirpes, não sendo possível observar nenhuma diferença,... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Rotavirus; Cytopathology; Cell culture; Plaque; Cytoskeleton. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000400002 |
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| Santos,Jucélia Stadinicki dos; Alfieri,Alice Fernandes; Leite,José Paulo Gagliardi; Skraba,Irene; Alfieri,Amauri Alcindo. |
| From January/2000 to December/2003, 550 diarrheic fecal samples from the children and adults were collected in several geographical regions of Paraná State, Brazil. The enzyme immunoassay showed 120 (21.8%) samples positive for the group A rotaviruses. One hundred and fourteen samples were genotyped by multiplex-nested-PCR assay. The highest frequency (77.5%) of the positive samples (n=93) was observed in the children under 5 years old. Rotavirus diarrhea was more frequent in the cold and dry seasons of the four evaluated years. The most frequent genotypes were: G1 (50.9%), G4 (9.6%), G9 (7.0%), G2 (1.7%), G3 (0.9%), P[ 8] (71.9%), and P[ 4] (3.5%). The P[ 8] G1 (46.5%) and P[ 8] G4 (9.6%) were the main combinations found to P and G genotypes. The mixed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrhea; Rotavirus; Diagnostic; PAGE; RT-PCR; Genotypes. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200008 |
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| Meneghetti,Ângelo Cesar; Bolognini,Andrea Maria; Lauretti,Flávio; Linhares,Rosa Elisa Carvalho; Santos,Norma; Nozawa,Carlos Mitihiko. |
| Rotaviruses are common pathogens and the causal agents of acute diarrhea among children and young animals. The involvement of rotavirus in human diarrheal disease among population of urban and rural areas of the city of Londrina, Parana was evaluated. Nine hundred and five fecal specimens from persons with diarrhea were studied, being 686 and 219 from urban and rural areas, respectively. Thirty-eight samples (4,2%) were positive for rotavirus by polyacrylamide gel electrophoresis of viral RNA and latex agglutination test of which 36 were from urban and two from rural areas. Out of the positive specimens, 17 strains were further characterized by RT-PCR typing assay, resulting in 16 strains of G1 genotype while one sample was found to be a mixture of G1 and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Diarrhea; PAGE; LA; PCR. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300006 |
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| Brandão,Paulo Eduardo; Cortez,Adriana; Ferreira,Fernando; Ferreira Neto,José Soares; Gregori,Fábio; Heinemann,Marcos Bryan; Homem,Valéria Stacchini Ferreira; Rodriguez,Cesar Alejandro Rosales; Jerez,José Antonio. |
| Rotavirus is a worldwide etiologic agent of diarrhea, responsible for large economic losses. We studied the seroprevalence of antibodies to group A rotavirus in cattle in 67 smallholder farms from Uruará municipality, using counterimmunoelectroosmophoresis with the NCDV strain as a standard antigen. Prevalence of positive smallholder farms was 95.6-100%. Significant differences were seen between age groups when the seropositivity rose from the youngest to the oldest groups and between females and males older than 1 year, when the seropositivity was higher in the first group. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Seroepidemiologic studies; Antibodies; Rotavirus; Cattle; Amazonia. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000100003 |
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| Langoni,Helio; Linhares,Antonio Carlos; Avila,Fernando Antonio de; Da Silva,Aristeu Vieira; Elias,Acacia Orieth. |
| Two hundred and three samples of feces of calves aged less than 30 days old from both sexes and from different properties and regions of the state of São Paulo were examined in the period of two years. Bacterial cultures were carried out in bovine 10% bloodagar and Levine mediums, incubated for up to 96 hours in aerobic conditions at 37°C, with the observation of colonies and morphological and biochemical study to characterize isolated microorganisms, or other tests, when pertinent. ELISA was performed for the Rotavirus research. The Cryptosporydium spp was surveyed and its parasitological examination was made. Results revealed the involvement of several enteropathogens alone and associated. Rotavirus was found in 51 (25.1%) samples, being 58.8% alone and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrhea; Calves; Etiology; Rotavirus; Cryptospodium. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000500004 |
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| Carraro,Emerson; Perosa,Ana Helena Sitta; Siqueira,Itacy; Pasternak,Jacyr; Martino,Marines Dalla Vale. |
| During the period of January 2003 to December 2005, 3,768 stool samples were received in the Microbiology Laboratory for rotavirus antigen detection from outpatients and inpatients of Albert Einstein Hospital, SP. Fresh stool samples from children and adults were analyzed by two methodologies: during 2003 and 2004 by latex agglutination (Slidex Rotavirus, Biomerieux) and 2005 by an immunochromatographic assay for the combined detection of rotavirus and adenovirus (Vikia Rota-Adeno, Biomerieux). Rotavirus group A was detected in 755 (20%) samples. The annual prevalence was 19.8% in 2003, 21.7% in 2004, and 18.7% in 2005. Rotavirus was detected every month during the period of the study, with peak of positivity between June and August (>35%). The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Gastroenteritis. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100010 |
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| Serravalle,Karina; Santos,Norma; Sardi,Silvia Inês; Silva,Sarah Peregrino Santos; Ribeiro Junior,Hugo da Costa; Mattos,Ângela Peixoto de; Campos,Gúbio Soares. |
| Rotavirus is a major cause of infectious diarrhea in infants and young children. The objective of this study was to characterize the genotypes of Human Rotavirus found in children hospitalized with acute diarrhea in the Pediatric Hospital Prof. Hosannah de Oliveira of the UFBA in Salvador, Bahia, Brazil, during the years of 1999, 2000 and 2002. Fecal samples were analyzed (n=358) by methods EIARA and SDS-PAGE for detection of Rotavirus. Positive samples of one or two of these methods (n=168) were submitted to RT-PCR and Multiplex-Nested PCR to determine genotypes G and P. A hundred sixty-eight (46.9%) samples were positive and 190 (53.1%) negative. Only 17 (4.7%) samples had divergent results. The distribution of genotypes G during the first year, showed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; PCR; Genotype; Children. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000100010 |
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| Nozawa,Carlos M; Kerntopf,Gustavo Franthesco; Czernisz,Érika da Silva; Albuquerque,Daniele; Romanin,Priscila; Freitas,José Felipe Eliseu; Santos,Norma; Benati,Fabrício José; Pietruchinski,Eduardo; Linhares,Rosa Elisa Carvalho. |
| Acute diarrheal disease is still one of the major public health problems worldwide. Rotaviruses (RV) are the most important viral etiologic agents and children under five years of age are the target population. OBJECTIVE: To investigate the rate of RV infection in hospitalized patients due to acute diarrhea in the cities of Ponta Grossa, Londrina and Assai - Paraná. METHODS: Latex agglutination (LA); immunochromatography (ICG); polyacrylamide gel electrophoresis (PAGE) and negative staining electron microscopy (ME) tests were used to detect the virus. For the genotyping, RT-PCR and RT-PCR-ELISA were used, respectively, for NSP4 and VP4/VP7. RESULT: Out of 124 samples there were 69 positive stool samples for RV, for at least one of the used tests, 67 of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Determination; Hospitalized patients; Genotyping. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600002 |
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| Carneiro,Nadya B.; Diniz-Santos,Daniel R.; Fagundes,Sabrina Q.; Neves,Lívio L.; Reges,Rodrigo M. B.; Lima,Eduardo K. P.; Quadros,Vitor H. de Oliva; Soares,Luis E. de Jesus; Silva,Fernando S. R.; Schneiter,Henrique de Gouvêa; Figueiredo,Ísis; Silva,Luciana R.. |
| Little is known about the epidemiology of severe rotavirus-associated gastroenteritis in Brazil. Given the morbidity associated with this condition and the importance of having detailed knowledge about the impact of rotavirus infection on the epidemiology of acute diarrhea in children, especially those with the most severe diarrheal conditions, we retrospectively reviewed the medical records of all pediatric patients admitted to a tertiary hospital in Salvador, Brazil, due to rotavirus-associated gastroenteritis during one year. It was observed that rotavirus was responsible for 15.6% of the hospitalizations caused by diarrhea and/or vomiting during the period of the study and that 87 of 218 (39.1%) patients seen at the emergency room with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Acute diarrhea; Epidemiology; Children. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000600013 |
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| Cardoso,D.D.P.; Rácz,M.L.; Azevedo,M.S.P.; Martins,R.M.B.; Soares,C.M.A.. |
| The G genotyping of 74 group A rotavirus samples was done by RNA-DNA hybridization (dot-blot) using oligonucleotide probes for the VP7 gene region of the human rotavirus serotypes/genotypes 1, 2, 3 and 4. Thirty-one samples could be genotyped by dot-blot showing the following results: G1 = 16, G4 = 6, G3 = 5, and G2 = 4. The data show circulation of genotypes G1-G4 and the predominance of G1. The knowledge of genotypes provides important information concerning rotavirus circulation in Central Brazil. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Rotavirus; G genotyping; VP7; Oligonucleotides. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400005 |
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| Guimarães,M.A.A.M.; Nozawa,C.M.; Guimarães,A.C.C.; Ramos,S.. |
| Rotaviruses and reoviruses are involved in human and animal diseases. It is known that both viruses penetrate the gastrointestinal tract but their interaction with phagocytic cells is unknown. To study this interaction, peritoneal resident phagocytic cells were used and rotavirus and reovirus replication in peritoneal phagocytic cells was observed. However, rotavirus replication in these cells led to the production of defective particles since MA-104 cells inoculated with rotavirus phagocytic cell lysate did not show any evidence of virus replication. On the basis of these results, we suggest that, although reovirus dissemination may be helped by these phagocytic cells, these cells may control rotavirus infection and probably contribute to the prevention... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Reovirus; Phagocytic cells. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997001000008 |
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| Rodrigues,J.; Acosta,V.C.; Candeias,J.M.G.; Souza,L.O.; Filho,F.J.C.. |
| In a one-year prospective study carried out to define the role of rotavirus and Escherichia coli in local childhood diarrhea, we determined the prevalence of both agents in 54 diarrheic children attending a health center in Botucatu. Diarrheogenic E. coli (DEC) strains were characterized by O:H serotyping, a search for virulence genetic markers, and assays of adherence to HEp-2 cells. Except for enteroaggregative E. coli (EAEC), no other DEC category was detected in the children's stools. Both EAEC and rotavirus were isolated from 22 of the 54 (41.0%) diarrheic children as single agents or in combination with other enteropathogens. However, when considering the presence of a single agent, EAEC was dominant and isolated from 20.4% of the patients, whereas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrhea; Rotavirus; Enteroaggregative E. coli; Escherichia coli; Seasonality. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001100008 |
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| Registros recuperados: 39 | |
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