|
|
|
|
|
| Forster,Karine M.; Hartwig,Daiane D.; Seixas,Fabiana K.; McBride,Alan J.A.; Monte,Leonardo G.; Recuero,Ana Lúcia C.; Brod,Claudiomar S.; Hartleben,Cláudia P.; Amaral,Marta; Dellagostin,Odir A.. |
| Pathogenic Leptospira spp. are the etiological agents of leptospirosis, an important disease of both humans and animals. In urban settings, L. interrogans serovars are the predominant cause of disease in humans. The purpose of this study was to characterize a novel Leptospira isolate recovered from an abandoned swimming pool. Molecular characterization through sequencing of the rpoB gene revealed 100% identity with L. interrogans and variable-number tandem-repeat (VNTR) analysis resulted in a banding pattern identical to L. interrogans serogroup Icterohaemorrhagiae, serovar Copenhageni or Icterohaemorrhagiae. The virulence of the strain was determined in a hamster model of lethal leptospirosis. The lethal dose 50% (LD50) was calculated to be two... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira; Leptospirosis; Virulent; VNTR; RpoB. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100024 |
| |
|
|
| Giacomazzi, Sophie; Leroi, Francoise; L'Henaff, Cecile; Joffraud, Jean-jacques. |
| Aims: To evaluate rpoB gene as a biomarker of microbial biodiversity associated to cold-smoked salmon by a novel nested-polymerase chain reaction/temporal temperature gradient gel electrophoresis (PCR/TTGE) technique applied on pure cultures of reference strains. Methods and Results: DNA obtained from pure cultures of reference strains was used in a succession of a first PCR amplification of rpoB fragment with degenerated nonclamped primers and a nested-PCR with nondegenerated clamped primers. PCR products were then applied on a TTGE gel in order to analyse strains profile. High quantity of nested-PCR products were obtained for each tested strain and TTGE profiles showed a good separation between the different reference bacteria and an easy way to... |
| Tipo: Text |
Palavras-chave: TTGE; RpoB; Nested PCR; Cold smoked salmon flora. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-579.pdf |
| |
|
|
|
