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Methicillin-resistant Staphylococcus aureus isolated from an intensive care unit in Minas Gerais, Brazil, over a six-year period BJID
Nascimento,Thiago C.; Diniz,Cláudio G.; Silva,Vânia L.; Ferreira-Machado,Alessandra B.; Fajardo,Marina O.; de Oliveira,Tamara Lopes R.; Ferreira,Dennis de C.; Cavalcante,Fernanda S.; Santos,Kátia R. Netto dos.
ABSTRACT To characterize methicillin-resistant Staphylococcus aureus isolates from an intensive care unit of a tertiary-care teaching hospital, between 2005 and 2010. A total of 45 isolates were recovered from patients admitted to the intensive care unit in the study period. Resistance rates higher than 80% were found for clindamycin (100%), erythromycin (100%), levofloxacin (100%), azithromycin (97.7%), rifampin (88.8%), and gentamycin (86.6%). The SCCmec typing revealed that the isolates harbored the types III (66.7%), II (17.8%), IV (4.4%), and I (2.2%). Four (8.9%) isolates carried non-typeable cassettes. Most (66.7%) of the isolates were related to the Brazilian endemic clone from CC8/SCCmec III, which was prevalent (89.3%) between 2005 and 2007,...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Clonal complex; MRSA; PFGE lineages; SCCmec.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000100055
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Epidemiological and molecular characterization of community and hospital acquired Staphylococcus aureus strains prevailing in Shenyang, Northeastern China BJID
Sun,Dan Dan; Ma,Xiao Xue; Hu,Jian; Tian,Yuan; Pang,Long; Shang,Hong; Cui,Long Zhu.
In order to obtain adequate information for the treatment of methicillin resistant Staphylococcus aureus (MRSA) infections, it is crucial to identify trends in epidemiological and antimicrobial resistance patterns of local S. aureus strains. Community and hospital acquired S. aureus isolates (n = 202) were characterized using staphylococcal cassette chromosome mec (SCCmec) typing, pulse field gel electrophoresis (PFGE) analysis, spa typing and minimal inhibitory concentration (MIC) determination. The prevalence of the Panton-Valentine leukocidine (pvl) and several antibiotic resistance genes among the isolates were also detected by PCR. All of the S. aureus isolates were susceptible to vancomycin, daptomycin and linezolid. Three hospital isolates were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CA-and HA-MRSA; SCCmec; PFGE; Spa; Pvl gene.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000600010
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Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from a Brazilian university hospital BJID
Martins,André; Riboli,Danilo Flávio Moraes; Pereira,Valéria Cataneli; Cunha,Maria de Lourdes Ribeiro de Souza da.
The aim of this study was to perform SCCmec typing in Staphylococcus aureus isolates and to characterize the clonal profile of these isolates. Forty-six mecA gene-positive strains isolated between 2002 and 2006 were submitted to antimicrobial resistance testing by the E-test, SCCmec typing by multiplex PCR, and clonal profile analysis by pulsed-field gel electrophoresis. Forty-one (89.1%) isolates were typed as SCCmec III and five (10.9%) as SCCmec IV. Four circulating clones were detected, one of them comprising isolates related to the Brazilian epidemic clone. This clone was detected throughout the study period. The SCCmec III isolates were associated with a high rate of multidrug resistance and clonal dissemination of methicillin-resistant S. aureus in...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Staphylococcus aureus; MRSA; SCCmec; BEC.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300331
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Community-acquired methicillin-resistant Staphylococcus aureus carrying SCCmec type IV and V isolated from healthy children attending public daycares in northeastern Brazil BJID
Carvalho,Suzi P. de; Almeida,Jéssica B. de; Andrade,Yasmin M.F.S.; Silva,Lucas S.C. da; Oliveira,Arianne C. de; Nascimento,Flávia S.; Campos,Guilherme B.; Oliveira,Márcio V.; Timenetsky,Jorge; Marques,Lucas M..
Abstract Nasal colonization with methicillin-resistant Staphylococcus aureus (MRSA) have increasingly been reported in healthy communities. This study aimed to assess the rate of S. aureus in general and MRSA in particular from nasal secretion of children in daycare centers in Vitória da Conquista, Brazil. The isolates were identified based on morphology, biochemical tests and by PCR. Detection of virulence genes, biofilm production, and susceptibility test by disk diffusion agar were performed. MRSA isolates were characterized by spa, SCCmec, and multilocus sequence typing (MLST). S. aureus were recovered from 70 (47.3%) of 148 children. Among the 11 MRSA strains (15.7%), two SCCmec types (IV and V) were detected. MLST identified four STs related to three...
Tipo: Info:eu-repo/semantics/other Palavras-chave: CA-MRSA; Antibiotic resistance; SCCmec; MLST.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000400464
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Mannitol-negative methicillin-resistant Staphylococcus aureus from nasal swab specimens in Brazil BJM
Santos,Danielle Caldeira Martins dos; Costa,Thaina Miranda da; Rabello,Renata Fernandes; Alves,Fábio Aguiar; Mondino,Silvia Susana Bona de.
The isolation of mannitol-negative methicillin-resistant Staphylococcus aureus from nasal swabs is reported. Among the 59 isolates, 9 (15%) isolates were mannitol-negative; all of these isolates were categorized as staphylococcal cassette chromosome mec (SCCmec) type IVa. This report emphasizes that mannitol fermentation on mannitol salt agar should not be used as the sole criterion when screening nasal swab specimens for S. aureus.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Mannitol-negative; MRSA; Nasal swab surveillance; SCCmec.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200531
Registros recuperados: 5
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