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| Registros recuperados: 33 | |
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| Zárate Castrejón, José Luis. |
| La senescencia foliar es la última etapa del desarrollo de la hoja, se caracteriza por una serie de cambios fisiológicos, bioquímicos y estructurales. Las hojas pierden gradualmente clorofila, se desorganiza la maquinaria fotosintética y se reciclan los productos de degradación de las macromoléculas hacia otras partes en crecimiento (hojas jóvenes, flores, frutos) u órganos de almacenamiento como el tubérculo en Solanum tuberosum. Se conoce poco acerca de la relación entre la senescencia foliar y proceso del llenado del tubérculo. El objetivo del presente trabajo fue estudiar la senescencia foliar en dos variedades de papa con diferente ciclo biológico y relacionarlo con el llenado del tubérculo. Se monitoreó el amarillamiento foliar en toda la planta a... |
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Palavras-chave: ADN; Cloroplastos; LSU; Rubisco; SDS-PAGE; Cloroplast; DNA; Maestría; Botánica. |
| Ano: 2010 |
URL: http://hdl.handle.net/10521/275 |
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| Yüzbasioglu,Ertugrul; Dadandi,Mehmet Yasar; Özcan,Servet. |
| In this study, phylogenetic relationships among 39 Phlomis taxa were investigated based on seed protein profiles produced by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). A total of 21 polypeptide bands were scored, of which, 19 were polymorphic among the taxa of the genus Phlomis. A distance matrix was generated from the similarity matrix which was computed by using Jaccard’s similarity coefficients, based on polymorphic bands and then an UPGMA tree was established through cluster analysis performed on the distance matrix. Genetic distances ranged from 0.00 to 0.50 within subsection Dendrophlomis; from 0.00 to 0.625 within subsection Gymnophlomis and from 0.00 to 0.769 within subsection Oxyphlomis. The UPGMA tree formed... |
| Tipo: Journal article |
Palavras-chave: Phlomis; Phlomoides; Phlomis taxa; SDS-PAGE; Seed proteins. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200002 |
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| Montagna,D.R.; Rigo,V; Ramayo,L.G.; Goldman,L.H.; Huguet,M.J.; Maceira,N; Jar,A.M.. |
| El objetivo de este trabajo fue caracterizar las inmunoglobulinas séricas y la IgY de la yema de huevo de ñandú, a través de las técnicas de electroforesis en acetato de celulosa, electroforesis en gel de poliacrilamida y Western-blot. Se estudiaron 19 ñandúes clínicamente sanos de 14 a 15 meses de edad, provenientes un criadero de Balcarce, Argentina. La electroforesis en acetato de celulosa de los sueros mostró la presencia de cuatro fracciones proteicas: albúmina, alfa, beta y gamma globulinas. La concentración de las gamma globulinas fue de 0,69 ± 0,18 g/dl, con un rango de 0,42-1,05 g/dl. La corrida electroforética de la IgY muestró que esta se ubica en la fracción gammaglobulínica. La electroforesis en gel de poliacrilamida evidenció, tanto en suero... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ãandú; Electroforesis; SDS-PAGE. |
| Ano: 2013 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982013000200002 |
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| Badhe,R. V.; Thomas,A. B.; Harer,S. L.; Deshpande,A. D.; Salvi,N.; Waghmare,A.. |
| Red scorpions Mesobuthus tamulus (Coconsis, Pocock) were obtained from different regions of West and South India (Ratnagiri, Chiplun and Ahmednagar from Maharashtra and Chennai from Tamil Nadu, respectively). Their venoms composition was analyzed using gel electrophoresis (SDS-PAGE). All venom samples shared six bands of 170, 80, 60, 57, 43, and 38 kDa molecular weights. Bands of 115 kDa and 51.5 kDa were characteristic of venoms obtained from red scorpions of Chiplun region, and the 26kDa band was absent in scorpion venom from Tamil Nadu. The separated protein band patterns suggest that the venoms from Ratnagiri, Ahmednagar and Tamil Nadu had high similarities in their biochemical composition but differed from that of Chiplun region. These data were also... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Red scorpion; SDS-PAGE; Mesobuthus tamulus. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400008 |
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| Vejayan,J; Shin Yee,L; Ponnudurai,G; Ambu,S; Ibrahim,I. |
| Snake venoms comprise a highly complex mixture of proteins, which requires for their characterization the use of versatile two-dimensional electrophoresis techniques. In the present study, venoms obtained from eight snakes (Ophiophagus hannah, Naja kaouthia, Naja sumatrana, Bungarus fasciatus, Trimeresurus sumatranus, Tropidolaemus wagleri, Enhydrina schistosa and Calloselasma rhodostoma) commonly found in Malaysia were separated based on two independent properties, isoelectric point (pI) and molecular weight (MW). Many differences in snake venoms at the inter-family, inter-subfamily, inter-genus and inter-species levels were revealed. Notably, proteins from individuals of the Viperidae family - Trimeresurus sumatranus, Tropidolaemus wagleri and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venom; Protein profile analysis; Two-dimensional gel electrophoresis; SDS-PAGE. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400013 |
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| De Vera, M.E; Sato, K; Oyong, G; Claveria, F.G. |
| Fasciola hepatica and Fasciola gigantica habitually co-exist as parasites of cattle (Bos taurus) and water buffalo (Bubalus bubalis), and despite variation in their morphometry, their species status is suspicious. Liver flukes isolated from cattle and water buffalo were initially sorted as F. gigantica or F. hepatica, and crude proteins were extracted and subjected to SDS-PAGE. Cattle had the preponderance of F. gigantica, while F. hepatica was the dominant species in water buffaloes. Co-existing cattle and bubaline fasciolids revealed similar protein profile suggestive of a close genetic relationship. The distinct heavy bands shared by co-existing bubaline F. hepatica and F. gigantica relative to those detected in cattle fasciolids suggests a host... |
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Palavras-chave: Cattle & carabao Fasciola spp; Protein profile; SDS-PAGE; Philippines. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2382 |
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| Pereira, Álvaro Júlio; UEM; Collet, Sandra Aparecida de Oliveira; UEM; Vidigal Filho, Pedro Soares; UEM; Machado, Maria de Fátima Pires da Silva; Biologia - UEM. |
| Proteins from young unexpanded leaves of seven cassava cultivars Manihot esculenta, Crantz (Euphorbidaceae) were investigated through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The comparison was made through the protein patterns obtained, and their relative amounts determined in plants leaves infected or not with the bacteria Xanthomonas axonopodis pv. manihotis. The electrophoretic protein pattern obtained from the investigated cultivars (“Fibra”, “Fécula Branca”, “Branca de Santa Catarina”, “Verdinha”, “IAC-12”, “IAC-13”, and “IAC-14”) showed a polypeptide subunit present exclusively in the “Fécula Branca” cultivar, with a molecular weight of 93.5 kDa. Also, protein fractions were more intensely stained in young leaves of M.... |
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Palavras-chave: 2.00.00.00-6 Ciências Biológicas cultivares de mandioca; Proteínas; SDS-PAGE; Patogênese em mandioca 2.00.00.00-6 Ciências Biológicas. |
| Ano: 2002 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2365 |
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| MEDICI,LEONARDO O.; GONÇALVES,FABÍOLA V.; FONSECA,MARCOS PAULO S. DA; GAZIOLA,SALETE A.; SCHMIDT,DAIANA; AZEVEDO,RICARDO A.; PIMENTEL,CARLOS. |
| ABSTRACT In this study, we are presenting recommendations to the best agricultural use as well as for plant breeding of three millet cultivars namely ENA1 and ENA2, which have African origin, and BRS1501 originally from India. These cultivars were evaluated for growth, yield and grain quality traits. The morphological traits evaluated in this study indicated that the African genotypes ENA1 and ENA2 are better than the Indian genotype BRS1501 for no-till farming or to produce forage with 15% of crude protein at flowering and at harvest to produce stover (around 7% of crude protein content) for livestock feeding. The BRS1501 cultivar exhibited the highest values for total crude protein, albumins and prolamins, phytate and mineral contents in grains. ENA1 and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mineral density; SDS-PAGE; Phytate; Seed storage proteins; Amino acids. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401749 |
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| Fagliari,J.J.; Rizolli,F.W.; Silva,S.L.; Silva,D.G.. |
| The serum protein concentration of newborn Holstein calves determined by means of sodium dodecyl sulphate-polyacrylamide (SDS-PAGE) was studied. Blood samples from 40 healthy newborn calves were obtained 48 hours after birth. Calves had been given 3 liters of colostrum within 2 hours after birth, following by dose corresponding by 15% of animal weight each 24 hours. The results showed three different proteinograms: 19 calves had 14 proteins with molecular weights (MW) ranging from 28,000 D to 170,000D (proteinogram 1); 11 calves had 14 proteins with MW ranging from 18,000 to 170,000 D (proteinogram 1); and 10 calves had 12 proteins with MW ranging from 28,000 D to 170,000 D (proteinogram 3). The three groups presented similar IgG levels. The highest serum... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Bezerro; Proteinograma; Eletroforese; SDS-PAGE. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300026 |
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| Juliano,R.S.; Fioravanti,M.C.S.; Fagliari,J.J.; Silva,P.C.; Silva,L.A.F.. |
| Estudou-se o perfil eletroforético das proteínas séricas de bovinos sadios da raça Curraleiro por meio da técnica de eletroforese em gel de acrilamida contendo dodecil sulfato de sódio. Utilizaram-se amostras de soro sanguíneo de 228 bovinos da raça Curraleiro, 51 machos e 177 fêmeas, com idades entre sete meses e 12 anos, pertencentes a dois rebanhos localizados nos Estados de Goiás e Tocantins. Foram quantificadas proteína total e concentração plasmática de fibrinogênio. Verificaram-se variações nas concentrações das diferentes frações proteicas. Foram detectadas 26 proteínas e identificadas 10 delas. A ceruloplasmina esteve ausente em 78,1% dos indivíduos, e a α-antitripsina não foi detectada em nenhum animal. Proteína total, globulina, IgA, IgG e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovino; Raça nativa; SDS-PAGE; Proteína; Fibrinogênio. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000300002 |
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| Chacur,M.G.M.; Martins,E.A.F.; Silva,A.A.; Guaberto,L.M.; Gabriel Filho,L.R.A.; Sanches,O.C.; Oba,E.. |
| Objetivou-se estudar a morfometria corpórea, as características do sêmen, o perfil proteico do plasma seminal em SDS-PAGE e a concentração sérica de testosterona em cervos-sambar (Cervus unicolor), criados em cativeiro, na estação reprodutiva da primavera. Quatro machos com idades entre 12 e 36 meses foram avaliados em quatro momentos, com intervalos de sete dias, com peso corpóreo (60,5 a 89,0kg), índice de massa corporal (93,07kg/m2 a 126,56kg/m2), volume do ejaculado (0,50±0,35mL a 0,75±0,28mL), motilidade espermática (87,75±4,78% a 90,00±7,07%), defeitos totais (17,25±5,81% a 47,72±17,55%), testosterona sérica (6,43±4,33ng/dL a 166,00±64,48ng/dL) e proteínas do plasma seminal com bandas entre 7,6 e 142kDa. As características dos ejaculados não... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cervidae; Cativeiro; Sêmen; SDS-PAGE; Andrógeno. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000501392 |
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| Santana,A.M.; Fagliari,J.J.; Camargo,C.M.S.; Santana,A.E.; Duarte,J.M.B.; Silva,P.R.L.. |
| The serum protein concentrations of brown brocket deer (Mazama gouazoubira) obtained by agarosis gel and sodium dodecyl sulphate-polyacrylamide (SDS-PAGE) gel were determined from blood samples of ten adult healthy animals (six females and four males), monthly collected in the morning, during 12 months. The animals, maintained in individual stable and protected from noise, received ad libitum a diet of comercial ration and green roughage. Serum protein concentrations in agarosis gel revealed the presence of four protein fractions: albumin, alphaglobulin, betaglobulin, and gammaglobulin. Only serum concentrations of albumin were influenced by season, being values in spring higher than values in summer (4.15 x 3.64g/dl). Serum concentrations of albumin (4.05... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Veado-catingueiro; Proteinograma; Eletroforese; SDS-PAGE. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000600039 |
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| Baptista,R.S.; Bovino,F.; Denadai,D.S.; Rahal,N.M.; Perri,S.H.V.; Lucas,F.A.; Feitosa,F.L.F.; Peiró,J.R.; Mendes,L.C.N.. |
| RESUMO O objetivo do estudo foi procurar proteínas de fase aguda que possam indicar sinais de maturação no neonato prematuro, por meio da quantificação sérica delas. Identificou-se a imunoglobulina A, a ceruloplasmina, a haptoglobina, a glicoproteína ácida, a transferrina, a albumina e as imunoglobulinas G de cadeias leve e pesada, pela comparação do perfil dos proteinogramas de cordeiros nascidos a termo com os prematuros submetidos a diferentes protocolos terapêuticos, a fim de estimular a atividade respiratória. Constituíram-se seis grupos: PN (n= 9): nascidos de parto normal; CN (n= 7): nascidos de cesariana em tempo normal de gestação; CP (n= 6): nascidos de cesariana prematura sem nenhum tipo de tratamento; DEX (n= 9): prematuros cujas mães receberam... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ovinos; Neonatologia; Eletroforese; SDS-PAGE; Proteinograma. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000401299 |
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| Registros recuperados: 33 | |
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