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| Berrocal,Liliana; Fuentes,Juan A; Nicole Trombert,A; Jofré,Matías R; Villagra,Nicolás A; Valenzuela,Luis M; Mora,Guido C. |
| BACKGROUND: Salmonella enterica serovar Typhi (S. Typhi) stg operon, encoding a chaperone/usher fimbria (CU), contributes to an increased adherence to human epithelial cells. However, one report suggests that the presence of the Stg fimbria impairs the monocyte-bacteria association, as deduced by the lower level of invasion to macrophage-like cells observed when the stg fimbrial cluster was overexpressed. Nevertheless, since other CU fimbrial structures increase the entry of S. Typhi into macrophages, and considering that transcriptomic analyses revealed that stg operon is indeed expressed in macrophages, we reassessed the role of the stg operon in the interaction between S. Typhi strain STH2370 and human cells, including macrophage-like cells and... |
| Tipo: Journal article |
Palavras-chave: Salmonella Typhi; Stg; Fimbriae; Adherence; Association bacteria-eukaryotic cells. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100034 |
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| Abdullah,J.; Saffie,N.; Sjasri,F.A.R.; Husin,A.; Abdul-Rahman,Z.; Ismail,A.; Aziah,I.; Mohamed,M.. |
| An in-house loop-mediated isothermal amplification (LAMP) reaction was established and evaluated for sensitivity and specificity in detecting the presence of Salmonella Typhi (S. Typhi) isolates from Kelantan, Malaysia. Three sets of primers consisting of two outer and 4 inner were designed based on locus STBHUCCB_38510 of chaperone PapD of S. Typhi genes. The reaction was optimised using genomic DNA of S. Typhi ATCC7251 as the template. The products were visualised directly by colour changes of the reaction. Positive results were indicated by green fluorescence and negative by orange colour. The test was further evaluated for specificity, sensitivity and application on field samples. The results were compared with those obtained by gold standard culture... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Loop-mediated isothermal amplification (LAMP); Salmonella Typhi. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400032 |
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