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| Horn, Karin; Ono, Kenichiro; Heydorn, Alfred Otto. |
| This paper describes a standard method for in vitro excystation of sporocysts of S.capracanis, S.hircicanis, S.ovicanis and S.arieticanis. A standard pretreatment was performed by 20 minutes preincubation of sporocysts in aqueous sodium hypochlorite (NaOCl) solution of 6% or 8% at room temperature. Then, sporocysts were washed 5 times in distilled water and were incubated for one hour at 39OC in excystation fluid consisting of RPMI 1640 medium, 10% PCS and 15% bovine bile. Additional sonication of pretreated sporocysts increased excystation rates as compared to not sonicated controls. Excystation rates for 1-9 months old sporocysts were 77% for S.capracanis, 77% for S.hircicanis, 72% for S.arieticanis and 92% for S.ovicanis. Sporozoite suspension as... |
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Palavras-chave: Cryopreservation; In vitro excystation; Sarcocystis spp.; Sporozoites. |
| Ano: 1991 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/154 |
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| Portella,Luiza Pires; Cadore,Gustavo Cauduro; Sangioni,Luis Antonio; Alves,Marta Elena Machado; Chemeris,Raiza; Brum,Larissa Picada; Vogel,Fernanda Silveira Flores. |
| ABSTRACT: Infections caused by protozoa belonging to the Sarcocystidae family have worldwide distribution and are common in ruminants, leading to considerable economic losses. This study evaluates Sarcocystis spp., Toxoplasma gondii and Neospora caninum infections in sheep from Southwest region of Rio Grande do Sul, Brazil. Myocardium samples of 80 sheep raised on extensive system were collected. Tissue cysts were detected by direct examination and presence of infective agents was confirmed by PCR. Macroscopic evaluation did not reveal changes, but direct microscopic examination showed cysts in 76.2% (61/80, 95% CI: 66.9 - 85.9) samples, and all cysts were morphologically similar to those caused by Sarcocystis tenella or Sarcocystis arieticanis. PCR... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sarcocystis spp.; Toxoplasma gondii; Neospora caninum; PCR; Diagnosis. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901613 |
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